Genes and Development, volume 34, issue 1-2, pages 87-98
Fork pausing complex engages topoisomerases at the replisome.
Publication type: Journal Article
Publication date: 2019-12-05
Journal:
Genes and Development
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 10.5
ISSN: 08909369, 15495477
PubMed ID:
31805522
Genetics
Developmental Biology
Abstract
Replication forks temporarily or terminally pause at hundreds of hard-to-replicate regions around the genome. A conserved pair of budding yeast replisome components Tof1-Csm3 (fission yeast Swi1-Swi3 and human TIMELESS-TIPIN) act as a "molecular brake" and promote fork slowdown at proteinaceous replication fork barriers (RFBs), while the accessory helicase Rrm3 assists the replisome in removing protein obstacles. Here we show that the Tof1-Csm3 complex promotes fork pausing independently of Rrm3 helicase by recruiting topoisomerase I (Top1) to the replisome. Topoisomerase II (Top2) partially compensates for the pausing decrease in cells when Top1 is lost from the replisome. The C terminus of Tof1 is specifically required for Top1 recruitment to the replisome and fork pausing but not for DNA replication checkpoint (DRC) activation. We propose that forks pause at proteinaceous RFBs through a "sTOP" mechanism ("slowing down with topoisomerases I-II"), which we show also contributes to protecting cells from topoisomerase-blocking agents.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shyian M. et al. Fork pausing complex engages topoisomerases at the replisome. // Genes and Development. 2019. Vol. 34. No. 1-2. pp. 87-98.
GOST all authors (up to 50)
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Shyian M., Albert B. B., Zupan A. M., Ivanitsa V., Charbonnet G., Dilg D., SHORE D. L. Fork pausing complex engages topoisomerases at the replisome. // Genes and Development. 2019. Vol. 34. No. 1-2. pp. 87-98.
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TY - JOUR
DO - 10.1101/gad.331868.119
UR - https://doi.org/10.1101%2Fgad.331868.119
TI - Fork pausing complex engages topoisomerases at the replisome.
T2 - Genes and Development
AU - Shyian, Maksym
AU - Albert, Benjamin B.
AU - Zupan, Andreja Moset
AU - Ivanitsa, Vitalii
AU - Charbonnet, Gabriel
AU - Dilg, Daniel
AU - SHORE, DAVID L.
PY - 2019
DA - 2019/12/05 00:00:00
PB - Cold Spring Harbor Laboratory
SP - 87-98
IS - 1-2
VL - 34
PMID - 31805522
SN - 0890-9369
SN - 1549-5477
ER -
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@article{2019_Shyian,
author = {Maksym Shyian and Benjamin B. Albert and Andreja Moset Zupan and Vitalii Ivanitsa and Gabriel Charbonnet and Daniel Dilg and DAVID L. SHORE},
title = {Fork pausing complex engages topoisomerases at the replisome.},
journal = {Genes and Development},
year = {2019},
volume = {34},
publisher = {Cold Spring Harbor Laboratory},
month = {dec},
url = {https://doi.org/10.1101%2Fgad.331868.119},
number = {1-2},
pages = {87--98},
doi = {10.1101/gad.331868.119}
}
Cite this
MLA
Copy
Shyian, Maksym, et al. “Fork pausing complex engages topoisomerases at the replisome..” Genes and Development, vol. 34, no. 1-2, Dec. 2019, pp. 87-98. https://doi.org/10.1101%2Fgad.331868.119.