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Genes and Development, volume 7, issue 7b, pages 1364-1376

Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation.

Collins K. 1
Greider Carol W. 2
1
 
Cold Spring Harbor Laboratory Cold Spring Harbor, New York, 11724
2
 
Cold, Spring Harbor Laboratory
Publication typeJournal Article
Publication date1993-07-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor10.5
ISSN08909369, 15495477
Genetics
Developmental Biology
Abstract
Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We have identified two activities of Tetrahymena telomerase in addition to the previously described processive elongation reaction: a 3'-5' nucleolytic cleavage of primer or product DNA and a nonprocessive mode of elongation. The nucleolytic cleavage activity removed residues not conforming to the telomeric repeat sequence from a primer 3' end, eliminating mismatch between DNA primer and RNA template sequences. Template-matched residues were also cleaved from primer or product DNA. Specific primer lengths, sequences, and concentrations stimulated cleavage and processive or nonprocessive elongation differentially. These newly identified activities suggest that telomerase may catalyze a range of telomere synthesis and repair functions and suggest mechanistic similarities between telomerase and RNA polymerase enzymes. On the basis of our results, we propose a model for telomerase primer binding, cleavage, and elongation.

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GOST |
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GOST Copy
Collins K., Greider C. W. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. // Genes and Development. 1993. Vol. 7. No. 7b. pp. 1364-1376.
GOST all authors (up to 50) Copy
Collins K., Greider C. W. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. // Genes and Development. 1993. Vol. 7. No. 7b. pp. 1364-1376.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1101/gad.7.7b.1364
UR - https://doi.org/10.1101%2Fgad.7.7b.1364
TI - Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation.
T2 - Genes and Development
AU - Collins, K.
AU - Greider, Carol W.
PY - 1993
DA - 1993/07/01 00:00:00
PB - Cold Spring Harbor Laboratory
SP - 1364-1376
IS - 7b
VL - 7
SN - 0890-9369
SN - 1549-5477
ER -
BibTex |
Cite this
BibTex Copy
@article{1993_Collins
author = {K. Collins and Carol W. Greider},
title = {Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation.},
journal = {Genes and Development},
year = {1993},
volume = {7},
publisher = {Cold Spring Harbor Laboratory},
month = {jul},
url = {https://doi.org/10.1101%2Fgad.7.7b.1364},
number = {7b},
pages = {1364--1376},
doi = {10.1101/gad.7.7b.1364}
}
MLA
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MLA Copy
Collins, K., and Carol W. Greider. “Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation..” Genes and Development, vol. 7, no. 7b, Jul. 1993, pp. 1364-1376. https://doi.org/10.1101%2Fgad.7.7b.1364.
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