Open Access
Microbial Biotechnology, volume 13, issue 4, pages 1254-1261
Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli
Komarova Ekaterina S
1, 2
,
Chervontseva Zoya S
2, 3
,
Osterman Ilya A
1, 2
,
Evfratov Sergey A
1
,
Rubtsova Maria
1, 2
,
Zatsepin Timofei S.
1, 2
,
Semashko Tatiana
4
,
Kostryukova Elena S.
4, 5
,
Bogdanov Alexey A.
1
,
GELFAND MICHAEL J.
2, 3, 6
,
Dontsova Olga A.
1, 2, 7
,
Sergiev Petr V.
1, 2
Publication type: Journal Article
Publication date: 2020-03-23
Journal:
Microbial Biotechnology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.7
ISSN: 17517907, 17517915
PubMed ID:
32202698
Biochemistry
Applied Microbiology and Biotechnology
Biotechnology
Bioengineering
Abstract
Summary
Translation efficiency contributes several orders of magnitude difference in the overall yield of exogenous gene expression in bacteria. In diverse bacteria, the translation initiation site, whose sequence is the primary determinant of the translation performance, is comprised of the start codon and the Shine–Dalgarno box located upstream. Here, we have examined how the sequence of a spacer between these main components of the translation initiation site contributes to the yield of synthesized protein. We have created a library of reporter constructs with the randomized spacer region, performed fluorescently activated cell sorting and applied next‐generation sequencing analysis (the FlowSeq protocol). As a result, we have identified sequence motifs for the spacer region between the Shine–Dalgarno box and AUG start codon that may modulate the translation efficiency in a 100‐fold range.
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1 publication, 4.76%
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1 publication, 4.76%
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Acta Naturae, 1, 4.76%
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1 publication, 4.76%
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1 publication, 4.76%
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1
2
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Citations by publishers
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1
2
3
4
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4 publications, 19.05%
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3 publications, 14.29%
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Springer Nature
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2 publications, 9.52%
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Elsevier
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2 publications, 9.52%
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1 publication, 4.76%
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Wiley
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1 publication, 4.76%
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1 publication, 4.76%
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Taylor & Francis
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1 publication, 4.76%
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1 publication, 4.76%
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1
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4
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Komarova E. S. et al. Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli // Microbial Biotechnology. 2020. Vol. 13. No. 4. pp. 1254-1261.
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Komarova E. S., Chervontseva Z. S., Osterman I. A., Evfratov S. A., Rubtsova M., Zatsepin T. S., Semashko T., Kostryukova E. S., Bogdanov A. A., GELFAND M. J., Dontsova O. A., Sergiev P. V. Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli // Microbial Biotechnology. 2020. Vol. 13. No. 4. pp. 1254-1261.
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TY - JOUR
DO - 10.1111/1751-7915.13561
UR - https://doi.org/10.1111%2F1751-7915.13561
TI - Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli
T2 - Microbial Biotechnology
AU - Chervontseva, Zoya S
AU - Evfratov, Sergey A
AU - Semashko, Tatiana
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
AU - Komarova, Ekaterina S
AU - Osterman, Ilya A
AU - Rubtsova, Maria
AU - Zatsepin, Timofei S.
AU - Kostryukova, Elena S.
AU - Bogdanov, Alexey A.
AU - GELFAND, MICHAEL J.
PY - 2020
DA - 2020/03/23 00:00:00
PB - Wiley
SP - 1254-1261
IS - 4
VL - 13
PMID - 32202698
SN - 1751-7907
SN - 1751-7915
ER -
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@article{2020_Komarova,
author = {Zoya S Chervontseva and Sergey A Evfratov and Tatiana Semashko and Olga A. Dontsova and Petr V. Sergiev and Ekaterina S Komarova and Ilya A Osterman and Maria Rubtsova and Timofei S. Zatsepin and Elena S. Kostryukova and Alexey A. Bogdanov and MICHAEL J. GELFAND},
title = {Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli},
journal = {Microbial Biotechnology},
year = {2020},
volume = {13},
publisher = {Wiley},
month = {mar},
url = {https://doi.org/10.1111%2F1751-7915.13561},
number = {4},
pages = {1254--1261},
doi = {10.1111/1751-7915.13561}
}
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MLA
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Komarova, Ekaterina S., et al. “Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine-tuning of the translation efficiency in Escherichia coli.” Microbial Biotechnology, vol. 13, no. 4, Mar. 2020, pp. 1254-1261. https://doi.org/10.1111%2F1751-7915.13561.