FEBS Journal, volume 289, issue 17, pages 5305-5321
PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes
2
Department of Protein Evolution Max Planck Institute for Developmental Biology Tübingen Germany
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Publication type: Journal Article
Publication date: 2022-03-24
Journal:
FEBS Journal
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 5.4
ISSN: 1742464X, 14321033
Biochemistry
Molecular Biology
Cell Biology
Abstract
PII proteins are signal processor proteins that regulate the cellular metabolism of Bacteria, Archea and plant chloroplasts typically in response to the cellular nitrogen status. Here, we report the first biochemical characterization of a novel PII-like protein PotN from Lentilactobacillus hilgardii. PotN is encoded in an operon together with the potABCD genes, encoding the ABC transporter for spermidine/putrescine. Like canonical PII proteins, the native PotN has a trimeric structure and competitively binds ATP and ADP, but it does not bind 2-oxoglutarate. Immunoprecipitation and pull-down experiments revealed that PotN is associated in vivo with the transcriptional regulator GlnR and the beta-subunit of pyruvate/2-oxoglutarate/acetoin dehydrogenase AcoB. Moreover, in vitro assays revealed that the ATPase domain of PotA also is able to interact with PotN. Interaction analyses demonstrated that PotN preferentially associates with PotA in the ADP state, whereas it binds to GlnR at elevated ATP levels. This suggests that PotN regulates the transport of polyamines and GlnR-dependent gene expression in response to the energy availability for the cell.
Citations by journals
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1
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Molecules
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Molecules
1 publication, 50%
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Proceedings of the National Academy of Sciences of the United States of America
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Proceedings of the National Academy of Sciences of the United States of America
1 publication, 50%
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1
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Citations by publishers
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1
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 50%
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Proceedings of the National Academy of Sciences (PNAS)
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Proceedings of the National Academy of Sciences (PNAS)
1 publication, 50%
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1
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Iskhakova Z. I. et al. PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes // FEBS Journal. 2022. Vol. 289. No. 17. pp. 5305-5321.
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Iskhakova Z. I., Zhuravleva D. E., Heim C., Hartmann M. D., Laikov A., Forchhammer K., Kayumov A. R. PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes // FEBS Journal. 2022. Vol. 289. No. 17. pp. 5305-5321.
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TY - JOUR
DO - 10.1111/febs.16431
UR - https://doi.org/10.1111%2Ffebs.16431
TI - PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes
T2 - FEBS Journal
AU - Iskhakova, Zalina I
AU - Zhuravleva, Darya E
AU - Heim, Christopher
AU - Hartmann, Marcus D.
AU - Laikov, Alexander
AU - Forchhammer, Karl
AU - Kayumov, Airat R.
PY - 2022
DA - 2022/03/24 00:00:00
PB - Wiley
SP - 5305-5321
IS - 17
VL - 289
SN - 1742-464X
SN - 1432-1033
ER -
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@article{2022_Iskhakova,
author = {Zalina I Iskhakova and Darya E Zhuravleva and Christopher Heim and Marcus D. Hartmann and Alexander Laikov and Karl Forchhammer and Airat R. Kayumov},
title = {PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes},
journal = {FEBS Journal},
year = {2022},
volume = {289},
publisher = {Wiley},
month = {mar},
url = {https://doi.org/10.1111%2Ffebs.16431},
number = {17},
pages = {5305--5321},
doi = {10.1111/febs.16431}
}
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MLA
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Iskhakova, Zalina I., et al. “PotN represents a novel energy‐state sensing PII subfamily, occurring in firmicutes.” FEBS Journal, vol. 289, no. 17, Mar. 2022, pp. 5305-5321. https://doi.org/10.1111%2Ffebs.16431.