Open Access
Science, volume 369, issue 6510, pages 1470-1476
90S pre-ribosome transformation into the primordial 40S subunit
Cheng Jingdong
1
,
Lau Benjamin
2
,
La Venuta Giuseppe
2
,
Ameismeier Michael
1
,
Berninghausen Otto
1
,
Hurt Ed
2
,
Beckmann Roland
1
1
Gene Center, Department of Biochemistry, University of Munich, 81377 Munich, Germany.
|
Publication type: Journal Article
Publication date: 2020-09-18
Multidisciplinary
Abstract
How ribosomes are made The formation of eukaryotic ribosomes is a complex process that starts with transcription of a large precursor RNA that assembles into a large 90S preribosome, which matures to finally give the 40S small subunit of the ribosome. Cheng et al. and Du et al. give insight into this process, using cryo–electron microscopy to look at intermediates along the pathway. Together, these studies reveal how a cast of molecular players act to coordinate the compositional and structural changes that transform the 90S preribosome into a pre-40S subunit. Science, this issue p. 1470, p. 1477 The steps that drive the stepwise dissociation of factors in the transition from the 90S to the 40S ribosome subunit are observed. Production of small ribosomal subunits initially requires the formation of a 90S precursor followed by an enigmatic process of restructuring into the primordial pre-40S subunit. We elucidate this process by biochemical and cryo–electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90S pre-ribosome, followed by Utp24 endonuclease–driven RNA cleavage at site A1, thereby separating the 5′-external transcribed spacer (ETS) from 18S ribosomal RNA. Next, the 5′-ETS and 90S assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40S emerges, still retaining some 90S factors including Dhr1, now ready to unwind the final small nucleolar U3–18S RNA hybrid. Our data shed light on the elusive 90S to pre-40S transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.
Citations by journals
|
1
2
3
4
5
6
7
|
|
|
Nucleic Acids Research
|
Nucleic Acids Research
7 publications, 11.86%
|
|
RNA Biology
|
RNA Biology
3 publications, 5.08%
|
|
Cell Reports
|
Cell Reports
2 publications, 3.39%
|
|
PLoS Genetics
|
PLoS Genetics
2 publications, 3.39%
|
|
Biological Chemistry
|
Biological Chemistry
2 publications, 3.39%
|
|
RNA
|
RNA
2 publications, 3.39%
|
|
Methods in Enzymology
|
Methods in Enzymology
2 publications, 3.39%
|
|
Nature
|
Nature
1 publication, 1.69%
|
|
Acta Naturae
|
Acta Naturae, 1, 1.69%
Acta Naturae
1 publication, 1.69%
|
|
Biochemical Society Transactions
|
Biochemical Society Transactions
1 publication, 1.69%
|
|
Journal of Cell Biology
|
Journal of Cell Biology
1 publication, 1.69%
|
|
Cells
|
Cells
1 publication, 1.69%
|
|
Non-coding RNA
|
Non-coding RNA
1 publication, 1.69%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 1.69%
|
|
Frontiers in Molecular Biosciences
|
Frontiers in Molecular Biosciences
1 publication, 1.69%
|
|
Current Genetics
|
Current Genetics
1 publication, 1.69%
|
|
Cell Death and Disease
|
Cell Death and Disease
1 publication, 1.69%
|
|
Nature Communications
|
Nature Communications
1 publication, 1.69%
|
|
Communications Biology
|
Communications Biology
1 publication, 1.69%
|
|
PLoS ONE
|
PLoS ONE
1 publication, 1.69%
|
|
Current Opinion in Structural Biology
|
Current Opinion in Structural Biology
1 publication, 1.69%
|
|
Methods
|
Methods
1 publication, 1.69%
|
|
Molecular Cell
|
Molecular Cell
1 publication, 1.69%
|
|
Journal of Biological Chemistry
|
Journal of Biological Chemistry
1 publication, 1.69%
|
|
Molecular Plant
|
Molecular Plant
1 publication, 1.69%
|
|
Current Biology
|
Current Biology
1 publication, 1.69%
|
|
Wiley interdisciplinary reviews. RNA
|
Wiley interdisciplinary reviews. RNA
1 publication, 1.69%
|
|
BioEssays
|
BioEssays
1 publication, 1.69%
|
|
eLife
|
eLife
1 publication, 1.69%
|
|
1
2
3
4
5
6
7
|
Citations by publishers
|
1
2
3
4
5
6
7
8
9
|
|
|
Elsevier
|
Elsevier
9 publications, 15.25%
|
|
Springer Nature
|
Springer Nature
7 publications, 11.86%
|
|
Oxford University Press
|
Oxford University Press
7 publications, 11.86%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 6.78%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
3 publications, 5.08%
|
|
Taylor & Francis
|
Taylor & Francis
3 publications, 5.08%
|
|
Wiley
|
Wiley
2 publications, 3.39%
|
|
Walter de Gruyter
|
Walter de Gruyter
2 publications, 3.39%
|
|
EMBO press
|
EMBO press
2 publications, 3.39%
|
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
2 publications, 3.39%
|
|
Acta Naturae Ltd
|
Acta Naturae Ltd, 1, 1.69%
Acta Naturae Ltd
1 publication, 1.69%
|
|
Portland Press
|
Portland Press
1 publication, 1.69%
|
|
Rockefeller University Press
|
Rockefeller University Press
1 publication, 1.69%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 1.69%
|
|
American Society for Biochemistry and Molecular Biology
|
American Society for Biochemistry and Molecular Biology
1 publication, 1.69%
|
|
eLife Sciences Publications
|
eLife Sciences Publications
1 publication, 1.69%
|
|
Genetics Society of America
|
Genetics Society of America
1 publication, 1.69%
|
|
American Association for the Advancement of Science (AAAS)
|
American Association for the Advancement of Science (AAAS)
1 publication, 1.69%
|
|
Proceedings of the National Academy of Sciences (PNAS)
|
Proceedings of the National Academy of Sciences (PNAS)
1 publication, 1.69%
|
|
1
2
3
4
5
6
7
8
9
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[3,18,22,16],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["5.08","30.51","37.29","27.12"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nucleic Acids Research","RNA Biology","Cell Reports","PLoS Genetics","Biological Chemistry","RNA","Methods in Enzymology","Nature","Acta Naturae","Biochemical Society Transactions","Journal of Cell Biology","Cells","Non-coding RNA","International Journal of Molecular Sciences","Frontiers in Molecular Biosciences","Current Genetics","Cell Death and Disease","Nature Communications","Communications Biology","PLoS ONE","Current Opinion in Structural Biology","Methods","Molecular Cell","Journal of Biological Chemistry","Molecular Plant","Current Biology","Wiley interdisciplinary reviews. RNA","BioEssays","eLife"],"ids":[23904,7613,9130,785,10443,12892,24566,14611,7401,10198,867,25293,18908,14627,3133,16818,25236,3231,11778,24963,13702,16671,19820,19451,8870,16010,8123,11017,24138],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp"],"datasets":[{"label":"","data":[7,3,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[11.86,5.08,3.39,3.39,3.39,3.39,3.39,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Springer Nature","Oxford University Press","Multidisciplinary Digital Publishing Institute (MDPI)","Public Library of Science (PLoS)","Taylor & Francis","Wiley","Walter de Gruyter","EMBO press","Cold Spring Harbor Laboratory","Acta Naturae Ltd","Portland Press","Rockefeller University Press","Frontiers Media S.A.","American Society for Biochemistry and Molecular Biology","eLife Sciences Publications","Genetics Society of America","American Association for the Advancement of Science (AAAS)","Proceedings of the National Academy of Sciences (PNAS)"],"ids":[17,8,19,202,344,18,11,4,9415,6909,7905,801,22,208,2090,6627,3356,189,162],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/9EVpRifnnUJt6oHGY5GfsfSXTEz5wzbdgnZqiF5m_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp"],"datasets":[{"label":"","data":[9,7,7,4,3,3,2,2,2,2,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[15.25,11.86,11.86,6.78,5.08,5.08,3.39,3.39,3.39,3.39,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69,1.69],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Cheng J. et al. 90S pre-ribosome transformation into the primordial 40S subunit // Science. 2020. Vol. 369. No. 6510. pp. 1470-1476.
GOST all authors (up to 50)
Copy
Cheng J., Lau B., La Venuta G., Ameismeier M., Berninghausen O., Hurt E., Beckmann R. 90S pre-ribosome transformation into the primordial 40S subunit // Science. 2020. Vol. 369. No. 6510. pp. 1470-1476.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1126/science.abb4119
UR - https://doi.org/10.1126%2Fscience.abb4119
TI - 90S pre-ribosome transformation into the primordial 40S subunit
T2 - Science
AU - Cheng, Jingdong
AU - Lau, Benjamin
AU - La Venuta, Giuseppe
AU - Ameismeier, Michael
AU - Berninghausen, Otto
AU - Hurt, Ed
AU - Beckmann, Roland
PY - 2020
DA - 2020/09/18 00:00:00
PB - American Association for the Advancement of Science (AAAS)
SP - 1470-1476
IS - 6510
VL - 369
SN - 0036-8075
SN - 1095-9203
ER -
Cite this
BibTex
Copy
@article{2020_Cheng,
author = {Jingdong Cheng and Benjamin Lau and Giuseppe La Venuta and Michael Ameismeier and Otto Berninghausen and Ed Hurt and Roland Beckmann},
title = {90S pre-ribosome transformation into the primordial 40S subunit},
journal = {Science},
year = {2020},
volume = {369},
publisher = {American Association for the Advancement of Science (AAAS)},
month = {sep},
url = {https://doi.org/10.1126%2Fscience.abb4119},
number = {6510},
pages = {1470--1476},
doi = {10.1126/science.abb4119}
}
Cite this
MLA
Copy
Cheng, Jingdong, et al. “90S pre-ribosome transformation into the primordial 40S subunit.” Science, vol. 369, no. 6510, Sep. 2020, pp. 1470-1476. https://doi.org/10.1126%2Fscience.abb4119.