Open Access

mBio, volume 10, issue 6

NusG-Dependent RNA Polymerase Pausing and Tylosin-Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis

Yakhnin Helen ^{1, 2}

Yakhnin Alexander V ^{1, 2}

Mouery Brandon L ^{1, 2}

Mandell Zachary F ^{1, 2}

Karbasiafshar Catherine ^{1, 2}

Kashlev Mikhail ³

Babitzke Paul ^{1, 2}

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA |

Center for RNA Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA |

RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA |

Publication type: Journal Article

Publication date: 2019-12-24

American Society for Microbiology

Journal: mBio

Quartile SCImago

Quartile WOS

Impact factor: 6.4

ISSN: 21612129, 21507511

DOI: 10.1128/mBio.02665-19

Copy DOI

PubMed ID: 31719185

Microbiology

Virology

Abstract

Antibiotic resistance is a growing health concern. Resistance mechanisms have evolved that provide bacteria with a growth advantage in their natural habitat such as the soil. We determined that B. subtilis , a Gram-positive soil organism, has a mechanism of resistance to tylosin, a macrolide antibiotic commonly used in the meat industry. Tylosin induces expression of yxjB , which encodes an enzyme that methylates 23S rRNA. YxjB-dependent methylation of 23S rRNA confers tylosin resistance. NusG-dependent RNA polymerase pausing and tylosin-dependent ribosome stalling induce yxjB expression, and hence tylosin resistance, by preventing transcription termination upstream of the yxjB coding sequence and by preventing repression of yxjB translation.

By date By citations

Citations by journals

	1 2
Journal of Biological Chemistry	Journal of Biological Chemistry, 2, 11.76% Journal of Biological Chemistry 2 publications, 11.76%
Wiley interdisciplinary reviews. RNA	Wiley interdisciplinary reviews. RNA, 2, 11.76% Wiley interdisciplinary reviews. RNA 2 publications, 11.76%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 2, 11.76% Proceedings of the National Academy of Sciences of the United States of America 2 publications, 11.76%
Biochemistry (Moscow)	Biochemistry (Moscow), 1, 5.88% Biochemistry (Moscow) 1 publication, 5.88%
Frontiers in Microbiology	Frontiers in Microbiology, 1, 5.88% Frontiers in Microbiology 1 publication, 5.88%
BMC Microbiology	BMC Microbiology, 1, 5.88% BMC Microbiology 1 publication, 5.88%
Nature	Nature, 1, 5.88% Nature 1 publication, 5.88%
Nature Communications	Nature Communications, 1, 5.88% Nature Communications 1 publication, 5.88%
Critical Reviews in Biochemistry and Molecular Biology	Critical Reviews in Biochemistry and Molecular Biology, 1, 5.88% Critical Reviews in Biochemistry and Molecular Biology 1 publication, 5.88%
Nucleic Acids Research	Nucleic Acids Research, 1, 5.88% Nucleic Acids Research 1 publication, 5.88%
Journal of Bacteriology	Journal of Bacteriology, 1, 5.88% Journal of Bacteriology 1 publication, 5.88%
Annual Review of Genetics	Annual Review of Genetics, 1, 5.88% Annual Review of Genetics 1 publication, 5.88%
Methods in Molecular Biology	Methods in Molecular Biology, 1, 5.88% Methods in Molecular Biology 1 publication, 5.88%
	1 2

Citations by publishers

	1 2 3 4
Springer Nature	Springer Nature, 4, 23.53% Springer Nature 4 publications, 23.53%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 2, 11.76% American Society for Biochemistry and Molecular Biology 2 publications, 11.76%
Wiley	Wiley, 2, 11.76% Wiley 2 publications, 11.76%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 2, 11.76% Proceedings of the National Academy of Sciences (PNAS) 2 publications, 11.76%
Pleiades Publishing	Pleiades Publishing, 1, 5.88% Pleiades Publishing 1 publication, 5.88%
Frontiers Media S.A.	Frontiers Media S.A., 1, 5.88% Frontiers Media S.A. 1 publication, 5.88%
Taylor & Francis	Taylor & Francis, 1, 5.88% Taylor & Francis 1 publication, 5.88%
Oxford University Press	Oxford University Press, 1, 5.88% Oxford University Press 1 publication, 5.88%
American Society for Microbiology	American Society for Microbiology, 1, 5.88% American Society for Microbiology 1 publication, 5.88%
Annual Reviews	Annual Reviews, 1, 5.88% Annual Reviews 1 publication, 5.88%
	1 2 3 4

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Yakhnin H. et al. NusG-Dependent RNA Polymerase Pausing and Tylosin-Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis // mBio. 2019. Vol. 10. No. 6.

GOST all authors (up to 50) Copy

Yakhnin H., Yakhnin A. V., Mouery B. L., Mandell Z. F., Karbasiafshar C., Kashlev M., Babitzke P. NusG-Dependent RNA Polymerase Pausing and Tylosin-Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis // mBio. 2019. Vol. 10. No. 6.

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RIS Copy

TY - JOUR

DO - 10.1128/mBio.02665-19

UR - https://doi.org/10.1128%2FmBio.02665-19

TI - NusG-Dependent RNA Polymerase Pausing and Tylosin-Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis

T2 - mBio

AU - Yakhnin, Helen

AU - Yakhnin, Alexander V

AU - Mouery, Brandon L

AU - Mandell, Zachary F

AU - Karbasiafshar, Catherine

AU - Kashlev, Mikhail

AU - Babitzke, Paul

PY - 2019

DA - 2019/12/24 00:00:00

PB - American Society for Microbiology

IS - 6

VL - 10

PMID - 31719185

SN - 2161-2129

SN - 2150-7511

ER -

BibTex

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@article{2019_Yakhnin,

author = {Helen Yakhnin and Alexander V Yakhnin and Brandon L Mouery and Zachary F Mandell and Catherine Karbasiafshar and Mikhail Kashlev and Paul Babitzke},

title = {NusG-Dependent RNA Polymerase Pausing and Tylosin-Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis},

journal = {mBio},

year = {2019},

volume = {10},

publisher = {American Society for Microbiology},

month = {dec},

url = {https://doi.org/10.1128%2FmBio.02665-19},

number = {6},

doi = {10.1128/mBio.02665-19}

}

Found error?

Publisher

American Society for Microbiology

Journal

mBio

Quartile SCImago

Quartile WOS

Impact factor

6.4

ISSN

21612129 (Print)
21507511 (Electronic)