Annual Review of Physiology, volume 79, issue 1, pages 93-117

A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging

Specht Elizabeth A 1, 2
Braselmann Esther 1, 2
Palmer Amy E. 1, 2
1
 
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80303;
2
 
BioFrontiers Institute, University of Colorado, Boulder, Colorado 80303
Publication typeJournal Article
Publication date2017-02-10
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor18.2
ISSN00664278, 15451585
Physiology
Abstract

Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at the cellular level. Fluorescent sensors have been developed on several platforms, utilizing either small-molecule dyes or fluorescent proteins, to monitor proteins, RNA, DNA, small molecules, and even cellular properties, such as pH and membrane potential. We briefly summarize the impressive history of tool development for these various applications and then discuss the most recent noteworthy developments in more detail. Particular emphasis is placed on tools suitable for single-cell analysis and especially live-cell imaging applications. Finally, we discuss prominent areas of need in future fluorescent tool development—specifically, advancing our capability to analyze and integrate the plethora of high-content data generated by fluorescence imaging.

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GOST |
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GOST Copy
Specht E. A. et al. A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging // Annual Review of Physiology. 2017. Vol. 79. No. 1. pp. 93-117.
GOST all authors (up to 50) Copy
Specht E. A., Braselmann E., Palmer A. A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging // Annual Review of Physiology. 2017. Vol. 79. No. 1. pp. 93-117.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1146/annurev-physiol-022516-034055
UR - https://doi.org/10.1146%2Fannurev-physiol-022516-034055
TI - A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging
T2 - Annual Review of Physiology
AU - Specht, Elizabeth A
AU - Braselmann, Esther
AU - Palmer, Amy E.
PY - 2017
DA - 2017/02/10 00:00:00
PB - Annual Reviews
SP - 93-117
IS - 1
VL - 79
SN - 0066-4278
SN - 1545-1585
ER -
BibTex |
Cite this
BibTex Copy
@article{2017_Specht
author = {Elizabeth A Specht and Esther Braselmann and Amy E. Palmer},
title = {A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging},
journal = {Annual Review of Physiology},
year = {2017},
volume = {79},
publisher = {Annual Reviews},
month = {feb},
url = {https://doi.org/10.1146%2Fannurev-physiol-022516-034055},
number = {1},
pages = {93--117},
doi = {10.1146/annurev-physiol-022516-034055}
}
MLA
Cite this
MLA Copy
Specht, Elizabeth A., et al. “A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.” Annual Review of Physiology, vol. 79, no. 1, Feb. 2017, pp. 93-117. https://doi.org/10.1146%2Fannurev-physiol-022516-034055.
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