Open Access
Blood, volume 124, issue 25, pages 3675-3684
An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity
1
Department of Biophysics and Biochemistry, University of California, San Francisco, San Francisco, CA
|
Publication type: Journal Article
Publication date: 2014-12-11
PubMed ID:
25320237
Biochemistry
Cell Biology
Immunology
Hematology
Abstract
Telomerase is a ribonucleoprotein complex that adds telomeric DNA to the ends of linear chromosomes. It contains two core canonical components: the essential RNA component, hTR, which provides the template for DNA synthesis, and the reverse transcriptase protein component, hTERT. Low telomerase activity in circulating peripheral blood mononuclear cells has been associated with a variety of diseases. It is unknown, however, whether telomerase, in addition to its long-term requirement for telomere maintenance, is also necessary for short-term immune cell proliferation and survival. We report that overexpression of enzymatically inactive hTR mutants protected against dexamethasone-induced apoptosis in stimulated CD4 T cells. Furthermore, hTR knockdown reproducibly induced apoptosis in the absence of any detectable telomere shortening or DNA damage response. In contrast, hTERT knockdown did not induce apoptosis. Strikingly, overexpression of hTERT protein caused apoptosis that was rescued by overexpression of enzymatically inactive hTR mutants. Hence, we propose that hTR can function as a noncoding RNA that protects from apoptosis independent of its function in telomerase enzymatic activity and long-term telomere maintenance in normal human immune cells. These results imply that genetic or environmental factors that alter hTR levels can directly affect immune cell function to influence health and disease.
Citations by journals
|
1
2
3
4
|
|
|
Nucleic Acids Research
|
Nucleic Acids Research
4 publications, 7.02%
|
|
Scientific Reports
|
Scientific Reports
3 publications, 5.26%
|
|
Oncotarget
|
Oncotarget
2 publications, 3.51%
|
|
Cancers
|
Cancers
2 publications, 3.51%
|
|
Frontiers in Cell and Developmental Biology
|
Frontiers in Cell and Developmental Biology
2 publications, 3.51%
|
|
PLoS ONE
|
PLoS ONE
2 publications, 3.51%
|
|
Psychoneuroendocrinology
|
Psychoneuroendocrinology
2 publications, 3.51%
|
|
Biomolecules
|
Biomolecules
1 publication, 1.75%
|
|
Biomedicines
|
Biomedicines
1 publication, 1.75%
|
|
Molecular Medicine Reports
|
Molecular Medicine Reports
1 publication, 1.75%
|
|
Viruses
|
Viruses
1 publication, 1.75%
|
|
Non-coding RNA
|
Non-coding RNA
1 publication, 1.75%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 1.75%
|
|
Frontiers in Genetics
|
Frontiers in Genetics
1 publication, 1.75%
|
|
Science China Life Sciences
|
Science China Life Sciences
1 publication, 1.75%
|
|
Experimental Gerontology
|
Experimental Gerontology
1 publication, 1.75%
|
|
Biological Research
|
Biological Research
1 publication, 1.75%
|
|
Biological Procedures Online
|
Biological Procedures Online
1 publication, 1.75%
|
|
Nature Reviews Urology
|
Nature Reviews Urology
1 publication, 1.75%
|
|
Oncogene
|
Oncogene
1 publication, 1.75%
|
|
Placenta
|
Placenta
1 publication, 1.75%
|
|
PLoS Pathogens
|
PLoS Pathogens
1 publication, 1.75%
|
|
Chemico-Biological Interactions
|
Chemico-Biological Interactions
1 publication, 1.75%
|
|
Cellular Immunology
|
Cellular Immunology
1 publication, 1.75%
|
|
Molecular Therapy - Nucleic Acids
|
Molecular Therapy - Nucleic Acids
1 publication, 1.75%
|
|
Cancer Letters
|
Cancer Letters
1 publication, 1.75%
|
|
Heart Lung and Circulation
|
Heart Lung and Circulation
1 publication, 1.75%
|
|
Journal of Leukocyte Biology
|
Journal of Leukocyte Biology
1 publication, 1.75%
|
|
Journal of Pathology
|
Journal of Pathology
1 publication, 1.75%
|
|
1
2
3
4
|
Citations by publishers
|
1
2
3
4
5
6
7
8
9
|
|
|
Elsevier
|
Elsevier
9 publications, 15.79%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
8 publications, 14.04%
|
|
Springer Nature
|
Springer Nature
8 publications, 14.04%
|
|
Wiley
|
Wiley
5 publications, 8.77%
|
|
Oxford University Press
|
Oxford University Press
4 publications, 7.02%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
4 publications, 7.02%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
3 publications, 5.26%
|
|
Impact Journals
|
Impact Journals
2 publications, 3.51%
|
|
Spandidos Publications
|
Spandidos Publications
1 publication, 1.75%
|
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
1 publication, 1.75%
|
|
|
, 1, 1.75%
1 publication, 1.75%
|
|
Taylor & Francis
|
Taylor & Francis
1 publication, 1.75%
|
|
American Association for Clinical Chemistry
|
American Association for Clinical Chemistry
1 publication, 1.75%
|
|
EMBO press
|
EMBO press
1 publication, 1.75%
|
|
American Association for Cancer Research (AACR)
|
American Association for Cancer Research (AACR)
1 publication, 1.75%
|
|
Proceedings of the National Academy of Sciences (PNAS)
|
Proceedings of the National Academy of Sciences (PNAS)
1 publication, 1.75%
|
|
eLife Sciences Publications
|
eLife Sciences Publications
1 publication, 1.75%
|
|
American Society for Microbiology
|
American Society for Microbiology
1 publication, 1.75%
|
|
1
2
3
4
5
6
7
8
9
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[5,3,3,8,11,7,8,7,4,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["8.77","5.26","5.26","14.04","19.3","12.28","14.04","12.28","7.02","1.75"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nucleic Acids Research","Scientific Reports","Oncotarget","Cancers","Frontiers in Cell and Developmental Biology","PLoS ONE","Psychoneuroendocrinology","Biomolecules","Biomedicines","Molecular Medicine Reports","Viruses","Non-coding RNA","International Journal of Molecular Sciences","Frontiers in Genetics","Science China Life Sciences","Experimental Gerontology","Biological Research","Biological Procedures Online","Nature Reviews Urology","Oncogene","Placenta","PLoS Pathogens","Chemico-Biological Interactions","Cellular Immunology","Molecular Therapy - Nucleic Acids","Cancer Letters","Heart Lung and Circulation","Journal of Leukocyte Biology","Journal of Pathology"],"ids":[23904,13767,8484,22471,12128,24963,6965,13780,24421,4737,2455,18908,14627,13242,8991,4210,1130,13047,15289,20423,17775,5093,15240,3786,20403,1654,3429,12074,24046],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/HY0CXgRt044tzIAB16PDvDzdHeKHGtSvlMH0yWf6_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[4,3,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[7.02,5.26,3.51,3.51,3.51,3.51,3.51,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature","Wiley","Oxford University Press","Frontiers Media S.A.","Public Library of Science (PLoS)","Impact Journals","Spandidos Publications","American Chemical Society (ACS)","","Taylor & Francis","American Association for Clinical Chemistry","EMBO press","American Association for Cancer Research (AACR)","Proceedings of the National Academy of Sciences (PNAS)","eLife Sciences Publications","American Society for Microbiology"],"ids":[17,202,8,11,19,208,344,342,1097,40,1305,18,2360,9415,2286,162,6627,142],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/HY0CXgRt044tzIAB16PDvDzdHeKHGtSvlMH0yWf6_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/9pZV06YbBPccLFla73CZaZzymKTwNAb1fmzIuWmR_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/cfuVw6XIq2mATDNJdXiose3W8qYbkmaVSpHoP8XL_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp"],"datasets":[{"label":"","data":[9,8,8,5,4,4,3,2,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[15.79,14.04,14.04,8.77,7.02,7.02,5.26,3.51,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75,1.75],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Gazzaniga F. S., Blackburn E. An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity // Blood. 2014. Vol. 124. No. 25. pp. 3675-3684.
GOST all authors (up to 50)
Copy
Gazzaniga F. S., Blackburn E. An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity // Blood. 2014. Vol. 124. No. 25. pp. 3675-3684.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1182/blood-2014-06-582254
UR - https://doi.org/10.1182%2Fblood-2014-06-582254
TI - An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity
T2 - Blood
AU - Gazzaniga, Francesca S
AU - Blackburn, Elizabeth
PY - 2014
DA - 2014/12/11 00:00:00
PB - American Society of Hematology
SP - 3675-3684
IS - 25
VL - 124
PMID - 25320237
SN - 0006-4971
SN - 1528-0020
ER -
Cite this
BibTex
Copy
@article{2014_Gazzaniga,
author = {Francesca S Gazzaniga and Elizabeth Blackburn},
title = {An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity},
journal = {Blood},
year = {2014},
volume = {124},
publisher = {American Society of Hematology},
month = {dec},
url = {https://doi.org/10.1182%2Fblood-2014-06-582254},
number = {25},
pages = {3675--3684},
doi = {10.1182/blood-2014-06-582254}
}
Cite this
MLA
Copy
Gazzaniga, Francesca S., and Elizabeth Blackburn. “An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity.” Blood, vol. 124, no. 25, Dec. 2014, pp. 3675-3684. https://doi.org/10.1182%2Fblood-2014-06-582254.