Open Access

BMC Genomics, volume 13, issue 1, publication number 255

Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes

Easwaran Sreesankar ¹

Ramamoorthy Senthilkumar ²

Bharathi Vellaichamy ²

Mishra Rakesh K ²

Mishra Krishnaveni ¹

Hide authors affiliations Show authors affiliations: 2 affiliations

Department of Biochemistry, School of Life sciences, University of Hyderabad, Hyderabad, India |

Centre for Cellular and Molecular Biology, Council of Scientific and industrial Research, Hyderabad, India |

Publication type: Journal Article

Publication date: 2012-06-19

Springer Nature

Journal: BMC Genomics

Quartile SCImago

Quartile WOS

Impact factor: 4.4

ISSN: 14712164

DOI: 10.1186/1471-2164-13-255

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PubMed ID: 22712556

Genetics

Biotechnology

Abstract

BackgroundTelomeres are nucleoprotein complexes at the end of linear eukaryotic chromosomes which maintain the genome integrity by regulating telomere length, preventing recombination and end to end fusion events. Multiple proteins associate with telomeres and function in concert to carry out these functions. Rap1 interacting factor 1 (Rif1), was identified as a protein involved in telomere length regulation in yeast. Rif1 is conserved upto mammals but its function has diversified from telomere length regulation to maintenance of genome integrity.ResultsWe have carried out detailed bioinformatic analyses and identified Rif1 homologues in 92 organisms from yeast to human. We identified Rif1 homologues in Drosophila melanogaster, even though fly telomeres are maintained by a telomerase independent pathway. Our analysis shows that Drosophila Rif1 (dRif1) sequence is phylogenetically closer to the one of vertebrates than yeast and has identified a few Rif1 specific motifs conserved through evolution. This includes a Rif1 family specific conserved region within the HEAT repeat domain and a motif involved in protein phosphatase1 docking. We show that dRif1 is nuclear localized with a prominent heterochromatin association and unlike human Rif1, it does not respond to DNA damage by localizing to damaged sites. To test the evolutionary conservation of dRif1 function, we expressed the dRif1 protein in yeast and HeLa cells. In yeast, dRif1 did not perturb yeast Rif1 (yRif1) functions; and in HeLa cells it did not colocalize with DNA damage foci.ConclusionsTelomeres are maintained by retrotransposons in all Drosophila species and consequently, telomerase and many of the telomere associated protein homologues are absent, including Rap1, which is the binding partner of Rif1. We found that a homologue of yRif1 protein is present in fly and dRif1 has evolutionarily conserved motifs. Functional studies show that dRif1 responds differently to DNA damage, implying that dRif1 may have a different function and this may be conserved in other organisms as well.

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International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 1.54% International Journal of Molecular Sciences 1 publication, 1.54%
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Genes	Genes, 1, 1.54% Genes 1 publication, 1.54%
Nature Structural and Molecular Biology	Nature Structural and Molecular Biology, 1, 1.54% Nature Structural and Molecular Biology 1 publication, 1.54%
PLoS Genetics	PLoS Genetics, 1, 1.54% PLoS Genetics 1 publication, 1.54%
PLoS Biology	PLoS Biology, 1, 1.54% PLoS Biology 1 publication, 1.54%
Biochemical and Biophysical Research Communications	Biochemical and Biophysical Research Communications, 1, 1.54% Biochemical and Biophysical Research Communications 1 publication, 1.54%
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EMBO Reports	EMBO Reports, 1, 1.54% EMBO Reports 1 publication, 1.54%
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Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 1, 1.54% Proceedings of the National Academy of Sciences of the United States of America 1 publication, 1.54%
Genes and Development	Genes and Development, 1, 1.54% Genes and Development 1 publication, 1.54%
Methods in Molecular Biology	Methods in Molecular Biology, 1, 1.54% Methods in Molecular Biology 1 publication, 1.54%
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Citations by publishers

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Elsevier	Elsevier, 15, 23.08% Elsevier 15 publications, 23.08%
Springer Nature	Springer Nature, 7, 10.77% Springer Nature 7 publications, 10.77%
Oxford University Press	Oxford University Press, 6, 9.23% Oxford University Press 6 publications, 9.23%
eLife Sciences Publications	eLife Sciences Publications, 4, 6.15% eLife Sciences Publications 4 publications, 6.15%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 4, 6.15% Multidisciplinary Digital Publishing Institute (MDPI) 4 publications, 6.15%
Frontiers Media S.A.	Frontiers Media S.A., 3, 4.62% Frontiers Media S.A. 3 publications, 4.62%
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Pleiades Publishing	Pleiades Publishing, 2, 3.08% Pleiades Publishing 2 publications, 3.08%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 2, 3.08% Public Library of Science (PLoS) 2 publications, 3.08%
Wiley	Wiley, 1, 1.54% Wiley 1 publication, 1.54%
Genetics Society of Japan/Nihon Iden Gakkai	Genetics Society of Japan/Nihon Iden Gakkai, 1, 1.54% Genetics Society of Japan/Nihon Iden Gakkai 1 publication, 1.54%
EMBO press	EMBO press, 1, 1.54% EMBO press 1 publication, 1.54%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 1, 1.54% Proceedings of the National Academy of Sciences (PNAS) 1 publication, 1.54%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 1, 1.54% Cold Spring Harbor Laboratory 1 publication, 1.54%
Taylor & Francis	Taylor & Francis, 1, 1.54% Taylor & Francis 1 publication, 1.54%
Rockefeller University Press	Rockefeller University Press, 1, 1.54% Rockefeller University Press 1 publication, 1.54%
	2 4 6 8 10 12 14 16

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Easwaran S. et al. Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes // BMC Genomics. 2012. Vol. 13. No. 1. 255

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Easwaran S., Ramamoorthy S., Bharathi V., Mishra R. K., Mishra K. Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes // BMC Genomics. 2012. Vol. 13. No. 1. 255

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TY - JOUR

DO - 10.1186/1471-2164-13-255

UR - https://doi.org/10.1186%2F1471-2164-13-255

TI - Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes

T2 - BMC Genomics

AU - Easwaran, Sreesankar

AU - Ramamoorthy, Senthilkumar

AU - Bharathi, Vellaichamy

AU - Mishra, Rakesh K

AU - Mishra, Krishnaveni

PY - 2012

DA - 2012/06/19 00:00:00

PB - Springer Nature

IS - 1

VL - 13

PMID - 22712556

SN - 1471-2164

ER -

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@article{2012_Easwaran,

author = {Sreesankar Easwaran and Senthilkumar Ramamoorthy and Vellaichamy Bharathi and Rakesh K Mishra and Krishnaveni Mishra},

title = {Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes},

journal = {BMC Genomics},

year = {2012},

volume = {13},

publisher = {Springer Nature},

month = {jun},

url = {https://doi.org/10.1186%2F1471-2164-13-255},

number = {1},

doi = {10.1186/1471-2164-13-255}

}

Found error?

Publisher

Springer Nature

Journal

BMC Genomics

Quartile SCImago

Quartile WOS

Impact factor

4.4

ISSN

14712164 (Print)