Open Access
Open access
BMC Biotechnology, volume 10, issue 1, publication number 43

Accurate non-invasive image-based cytotoxicity assays for cultured cells

Marqués Gallego Patricia 1
Den Dulk Hans 1
Backendorf Claude 1
Brouwer Jaap 1
Reedijk Jan 1
Burke Julian F. 2
1
 
Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, RA Leiden, The Netherlands
2
 
Genetix Limited, Queensway, New Milton, UK
Publication typeJournal Article
Publication date2010-06-17
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor3.5
ISSN14726750
Biotechnology
Abstract
BackgroundThe CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.ResultsHere we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.ConclusionsThis new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.

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Marqués Gallego P. et al. Accurate non-invasive image-based cytotoxicity assays for cultured cells // BMC Biotechnology. 2010. Vol. 10. No. 1. 43
GOST all authors (up to 50) Copy
Marqués Gallego P., Den Dulk H., Backendorf C., Brouwer J., Reedijk J., Burke J. F. Accurate non-invasive image-based cytotoxicity assays for cultured cells // BMC Biotechnology. 2010. Vol. 10. No. 1. 43
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RIS Copy
TY - JOUR
DO - 10.1186/1472-6750-10-43
UR - https://doi.org/10.1186%2F1472-6750-10-43
TI - Accurate non-invasive image-based cytotoxicity assays for cultured cells
T2 - BMC Biotechnology
AU - Marqués Gallego, Patricia
AU - Den Dulk, Hans
AU - Backendorf, Claude
AU - Brouwer, Jaap
AU - Reedijk, Jan
AU - Burke, Julian F.
PY - 2010
DA - 2010/06/17 00:00:00
PB - Springer Nature
IS - 1
VL - 10
PMID - 20565747
SN - 1472-6750
ER -
BibTex
Cite this
BibTex Copy
@article{2010_Marqués Gallego
author = {Patricia Marqués Gallego and Hans Den Dulk and Claude Backendorf and Jaap Brouwer and Jan Reedijk and Julian F. Burke},
title = {Accurate non-invasive image-based cytotoxicity assays for cultured cells},
journal = {BMC Biotechnology},
year = {2010},
volume = {10},
publisher = {Springer Nature},
month = {jun},
url = {https://doi.org/10.1186%2F1472-6750-10-43},
number = {1},
doi = {10.1186/1472-6750-10-43}
}
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