Open Access
Cell Division, volume 11, issue 1, publication number 3
Mitochondria-cytoskeleton associations in mammalian cytokinesis
1
Department of Anatomy and Cell Biology, McGill University, Montreal, Canada
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Publication type: Journal Article
Publication date: 2016-03-29
PubMed ID:
27030796
Biochemistry
Molecular Biology
Cell Biology
Abstract
The role of the cytoskeleton in regulating mitochondrial distribution in dividing mammalian cells is poorly understood. We previously demonstrated that mitochondria are transported to the cleavage furrow during cytokinesis in a microtubule-dependent manner. However, the exact subset of spindle microtubules and molecular machinery involved remains unknown. We employed quantitative imaging techniques and structured illumination microscopy to analyse the spatial and temporal relationship of mitochondria with microtubules and actin of the contractile ring during cytokinesis in HeLa cells. Superresolution microscopy revealed that mitochondria were associated with astral microtubules of the mitotic spindle in cytokinetic cells. Dominant-negative mutants of KIF5B, the heavy chain of kinesin-1 motor, and of Miro-1 disrupted mitochondrial transport to the furrow. Live imaging revealed that mitochondrial enrichment at the cell equator occurred simultaneously with the appearance of the contractile ring in cytokinesis. Inhibiting RhoA activity and contractile ring assembly with C3 transferase, caused mitochondrial mislocalisation during division. Taken together, the data suggest a model in which mitochondria are transported by a microtubule-mediated mechanism involving equatorial astral microtubules, Miro-1, and KIF5B to the nascent actomyosin contractile ring in cytokinesis.
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Lawrence E. J., Boucher E., Mandato C. Mitochondria-cytoskeleton associations in mammalian cytokinesis // Cell Division. 2016. Vol. 11. No. 1. 3
GOST all authors (up to 50)
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Lawrence E. J., Boucher E., Mandato C. Mitochondria-cytoskeleton associations in mammalian cytokinesis // Cell Division. 2016. Vol. 11. No. 1. 3
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TY - JOUR
DO - 10.1186/s13008-016-0015-4
UR - https://doi.org/10.1186%2Fs13008-016-0015-4
TI - Mitochondria-cytoskeleton associations in mammalian cytokinesis
T2 - Cell Division
AU - Lawrence, E. J.
AU - Boucher, E.
AU - Mandato, C.A.
PY - 2016
DA - 2016/03/29 00:00:00
PB - Springer Nature
IS - 1
VL - 11
PMID - 27030796
SN - 1747-1028
ER -
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@article{2016_Lawrence,
author = {E. J. Lawrence and E. Boucher and C.A. Mandato},
title = {Mitochondria-cytoskeleton associations in mammalian cytokinesis},
journal = {Cell Division},
year = {2016},
volume = {11},
publisher = {Springer Nature},
month = {mar},
url = {https://doi.org/10.1186%2Fs13008-016-0015-4},
number = {1},
doi = {10.1186/s13008-016-0015-4}
}