Open Access
Journal of Biological Engineering, volume 13, issue 1, publication number 63
Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates
1
Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, USA
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Publication type: Journal Article
Publication date: 2019-07-10
Journal:
Journal of Biological Engineering
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 17541611
PubMed ID:
31333759
Molecular Biology
Cell Biology
Environmental Engineering
Biomedical Engineering
Abstract
An adapted strategy from the conventional 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) crosslinking method was developed to form a covalently coupled phosphoramidated single stranded DNA (ssDNA). Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) results demonstrated that the phosphoramidated ssDNA conjugate is stable for several days, and that phosphoramidation occurred exclusively at the 5′ phosphate of ssDNA. A reversed phase high-performance liquid chromatography (RP-HPLC) method with UV detection was developed to determine the yield of conjugates. The methods coefficients of variation (%CV) were less than 6%, and biases ranged from − 5.1 – 1.2%. The conjugate yield via the conventional EDC method was 68.3 ± 2.2%, while that of the adapted EDC/Imidazole method was 79.0 ± 2.4% (n = 10). This study demonstrates a convenient one pot strategy for crosslinking biological molecules.
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Citations by publishers
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7 publications, 11.86%
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Wiley
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7 publications, 11.86%
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6 publications, 10.17%
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Springer Nature
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3 publications, 5.08%
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1 publication, 1.69%
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Belarusian State University
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Belarusian State University
1 publication, 1.69%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Wickramathilaka M. P., Tao B. Y. Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates // Journal of Biological Engineering. 2019. Vol. 13. No. 1. 63
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Wickramathilaka M. P., Tao B. Y. Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates // Journal of Biological Engineering. 2019. Vol. 13. No. 1. 63
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TY - JOUR
DO - 10.1186/s13036-019-0191-2
UR - https://doi.org/10.1186%2Fs13036-019-0191-2
TI - Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates
T2 - Journal of Biological Engineering
AU - Wickramathilaka, Malithi P
AU - Tao, Bernard Y.
PY - 2019
DA - 2019/07/10 00:00:00
PB - Springer Nature
IS - 1
VL - 13
PMID - 31333759
SN - 1754-1611
ER -
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@article{2019_Wickramathilaka,
author = {Malithi P Wickramathilaka and Bernard Y. Tao},
title = {Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates},
journal = {Journal of Biological Engineering},
year = {2019},
volume = {13},
publisher = {Springer Nature},
month = {jul},
url = {https://doi.org/10.1186%2Fs13036-019-0191-2},
number = {1},
doi = {10.1186/s13036-019-0191-2}
}