Open Access

volume 16, issue 1, publication number 20

Localization and abundance analysis of human lncRNAs at single-cell and single-molecule resolution

Cabili Moran N ^{1, 2, 3}

Dunagin Margaret C ⁴

Mcclanahan Patrick D ⁴

Biaesch Andrew ⁴

Padovan Merhar Olivia ⁴

Regev Aviv ^{1, 5}

Rinn John L. ^{1, 3}

Raj Arjun ⁴

Hide authors affiliations Show authors affiliations: 5 affiliations

Broad Institute of MIT and Harvard, Cambridge, USA |

Department of Systems Biology, Harvard Medical School, Boston, USA |

Department of Stem cell and Regenerative Biology, Harvard University, Cambridge, USA |

⁴

School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, USA |

⁵

Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, USA |

Publication type: Journal Article

Publication date: 2015-01-29

—

DOI: 10.1186/s13059-015-0586-4

Copy DOI

PubMed ID: 25630241

Abstract

BackgroundLong non-coding RNAs (lncRNAs) have been implicated in diverse biological processes. In contrast to extensive genomic annotation of lncRNA transcripts, far fewer have been characterized for subcellular localization and cell-to-cell variability. Addressing this requires systematic, direct visualization of lncRNAs in single cells at single-molecule resolution.ResultsWe use single-molecule RNA-FISH to systematically quantify and categorize the subcellular localization patterns of a representative set of 61 lncRNAs in three different cell types. Our survey yields high-resolution quantification and stringent validation of the number and spatial positions of these lncRNA, with an mRNA set for comparison. Using this highly quantitative image-based dataset, we observe a variety of subcellular localization patterns, ranging from bright sub-nuclear foci to almost exclusively cytoplasmic localization. We also find that the low abundance of lncRNAs observed from cell population measurements cannot be explained by high expression in a small subset of ‘jackpot’ cells. Additionally, nuclear lncRNA foci dissolve during mitosis and become widely dispersed, suggesting these lncRNAs are not mitotic bookmarking factors. Moreover, we see that divergently transcribed lncRNAs do not always correlate with their cognate mRNA, nor do they have a characteristic localization pattern.ConclusionsOur systematic, high-resolution survey of lncRNA localization reveals aspects of lncRNAs that are similar to mRNAs, such as cell-to-cell variability, but also several distinct properties. These characteristics may correspond to particular functional roles. Our study also provides a quantitative description of lncRNAs at the single-cell level and a universally applicable framework for future study and validation of lncRNAs.

By date By citations

Citations by journals

	2 4 6 8 10 12 14 16 18 20
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 19, 3.53% International Journal of Molecular Sciences 19 publications, 3.53%
Scientific Reports	Scientific Reports, 17, 3.15% Scientific Reports 17 publications, 3.15%
Nature Communications	Nature Communications, 13, 2.41% Nature Communications 13 publications, 2.41%
Methods in Molecular Biology	Methods in Molecular Biology, 12, 2.23% Methods in Molecular Biology 12 publications, 2.23%
Nucleic Acids Research	Nucleic Acids Research, 11, 2.04% Nucleic Acids Research 11 publications, 2.04%
Non-coding RNA	Non-coding RNA, 9, 1.67% Non-coding RNA 9 publications, 1.67%
RNA Biology	RNA Biology, 9, 1.67% RNA Biology 9 publications, 1.67%
Wiley interdisciplinary reviews. RNA	Wiley interdisciplinary reviews. RNA, 8, 1.48% Wiley interdisciplinary reviews. RNA 8 publications, 1.48%
Cancers	Cancers, 7, 1.3% Cancers 7 publications, 1.3%
Frontiers in Oncology	Frontiers in Oncology, 7, 1.3% Frontiers in Oncology 7 publications, 1.3%
Frontiers in Genetics	Frontiers in Genetics, 6, 1.11% Frontiers in Genetics 6 publications, 1.11%
Nature Reviews Genetics	Nature Reviews Genetics, 6, 1.11% Nature Reviews Genetics 6 publications, 1.11%
Non-coding RNA Research	Non-coding RNA Research, 6, 1.11% Non-coding RNA Research 6 publications, 1.11%
Briefings in Bioinformatics	Briefings in Bioinformatics, 6, 1.11% Briefings in Bioinformatics 6 publications, 1.11%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 6, 1.11% Proceedings of the National Academy of Sciences of the United States of America 6 publications, 1.11%
eLife	eLife, 6, 1.11% eLife 6 publications, 1.11%
Cold Spring Harbor perspectives in biology	Cold Spring Harbor perspectives in biology, 6, 1.11% Cold Spring Harbor perspectives in biology 6 publications, 1.11%
Frontiers in Cell and Developmental Biology	Frontiers in Cell and Developmental Biology, 5, 0.93% Frontiers in Cell and Developmental Biology 5 publications, 0.93%
Nature Methods	Nature Methods, 5, 0.93% Nature Methods 5 publications, 0.93%
Trends in Genetics	Trends in Genetics, 5, 0.93% Trends in Genetics 5 publications, 0.93%
Cell	Cell, 5, 0.93% Cell 5 publications, 0.93%
Frontiers in Endocrinology	Frontiers in Endocrinology, 4, 0.74% Frontiers in Endocrinology 4 publications, 0.74%
Cells	Cells, 4, 0.74% Cells 4 publications, 0.74%
Cell Reports	Cell Reports, 4, 0.74% Cell Reports 4 publications, 0.74%
PLoS ONE	PLoS ONE, 4, 0.74% PLoS ONE 4 publications, 0.74%
Genome Research	Genome Research, 4, 0.74% Genome Research 4 publications, 0.74%
RNA	RNA, 4, 0.74% RNA 4 publications, 0.74%
Molecules	Molecules, 3, 0.56% Molecules 3 publications, 0.56%
Frontiers in Immunology	Frontiers in Immunology, 3, 0.56% Frontiers in Immunology 3 publications, 0.56%
	2 4 6 8 10 12 14 16 18 20

Citations by publishers

	20 40 60 80 100 120
Springer Nature	Springer Nature, 110, 20.41% Springer Nature 110 publications, 20.41%
Elsevier	Elsevier, 82, 15.21% Elsevier 82 publications, 15.21%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 47, 8.72% Multidisciplinary Digital Publishing Institute (MDPI) 47 publications, 8.72%
Frontiers Media S.A.	Frontiers Media S.A., 35, 6.49% Frontiers Media S.A. 35 publications, 6.49%
Oxford University Press	Oxford University Press, 31, 5.75% Oxford University Press 31 publications, 5.75%
Wiley	Wiley, 31, 5.75% Wiley 31 publications, 5.75%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 17, 3.15% Cold Spring Harbor Laboratory 17 publications, 3.15%
Taylor & Francis	Taylor & Francis, 16, 2.97% Taylor & Francis 16 publications, 2.97%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 7, 1.3% Public Library of Science (PLoS) 7 publications, 1.3%
KeAi Communications Co.	KeAi Communications Co., 6, 1.11% KeAi Communications Co. 6 publications, 1.11%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 6, 1.11% Proceedings of the National Academy of Sciences (PNAS) 6 publications, 1.11%
eLife Sciences Publications	eLife Sciences Publications, 6, 1.11% eLife Sciences Publications 6 publications, 1.11%
Annual Reviews	Annual Reviews, 6, 1.11% Annual Reviews 6 publications, 1.11%
Portland Press	Portland Press, 5, 0.93% Portland Press 5 publications, 0.93%
Bentham Science	Bentham Science, 3, 0.56% Bentham Science 3 publications, 0.56%
Spandidos Publications	Spandidos Publications, 3, 0.56% Spandidos Publications 3 publications, 0.56%
SAGE	SAGE, 3, 0.56% SAGE 3 publications, 0.56%
The American Association of Immunologists	The American Association of Immunologists, 2, 0.37% The American Association of Immunologists 2 publications, 0.37%
American Society for Pharmacology and Experimental Therapeutics	American Society for Pharmacology and Experimental Therapeutics, 2, 0.37% American Society for Pharmacology and Experimental Therapeutics 2 publications, 0.37%
The Company of Biologists	The Company of Biologists, 2, 0.37% The Company of Biologists 2 publications, 0.37%
Wolters Kluwer Health	Wolters Kluwer Health, 2, 0.37% Wolters Kluwer Health 2 publications, 0.37%
Baishideng Publishing Group	Baishideng Publishing Group, 2, 0.37% Baishideng Publishing Group 2 publications, 0.37%
Federation of American Societies for Experimental Biology (FASEB)	Federation of American Societies for Experimental Biology (FASEB), 2, 0.37% Federation of American Societies for Experimental Biology (FASEB) 2 publications, 0.37%
American Chemical Society (ACS)	American Chemical Society (ACS), 2, 0.37% American Chemical Society (ACS) 2 publications, 0.37%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 2, 0.37% Royal Society of Chemistry (RSC) 2 publications, 0.37%
EMBO press	EMBO press, 2, 0.37% EMBO press 2 publications, 0.37%
Genetics Society of America	Genetics Society of America, 2, 0.37% Genetics Society of America 2 publications, 0.37%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 2, 0.37% American Association for the Advancement of Science (AAAS) 2 publications, 0.37%
Bio-Protocol	Bio-Protocol, 2, 0.37% Bio-Protocol 2 publications, 0.37%
	20 40 60 80 100 120

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[11,49,60,64,60,63,93,71,58,10],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["2.04","9.09","11.13","11.87","11.13","11.69","17.25","13.17","10.76","1.86"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","Scientific Reports","Nature Communications","Methods in Molecular Biology","Nucleic Acids Research","Non-coding RNA","RNA Biology","Wiley interdisciplinary reviews. RNA","Cancers","Frontiers in Oncology","Frontiers in Genetics","Nature Reviews Genetics","Non-coding RNA Research","Briefings in Bioinformatics","Proceedings of the National Academy of Sciences of the United States of America","eLife","Cold Spring Harbor perspectives in biology","Frontiers in Cell and Developmental Biology","Nature Methods","Trends in Genetics","Cell","Frontiers in Endocrinology","Cells","Cell Reports","PLoS ONE","Genome Research","RNA","Molecules","Frontiers in Immunology"],"ids":[14627,13767,3231,3516,23904,18908,7613,8123,22471,10990,13242,13955,7055,23409,306,24138,21200,12128,14446,14739,16552,19896,25293,9130,24963,9342,12892,1770,1699],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/jeWDR7NTA9Ab5MVz8Uqjl2RPCndzFjSyl0FaRAHg_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp"],"datasets":[{"label":"","data":[19,17,13,12,11,9,9,8,7,7,6,6,6,6,6,6,6,5,5,5,5,4,4,4,4,4,4,3,3],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[3.53,3.15,2.41,2.23,2.04,1.67,1.67,1.48,1.3,1.3,1.11,1.11,1.11,1.11,1.11,1.11,1.11,0.93,0.93,0.93,0.93,0.74,0.74,0.74,0.74,0.74,0.74,0.56,0.56],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Frontiers Media S.A.","Oxford University Press","Wiley","Cold Spring Harbor Laboratory","Taylor & Francis","Public Library of Science (PLoS)","KeAi Communications Co.","Proceedings of the National Academy of Sciences (PNAS)","eLife Sciences Publications","Annual Reviews","Portland Press","Bentham Science","Spandidos Publications","SAGE","The American Association of Immunologists","American Society for Pharmacology and Experimental Therapeutics","The Company of Biologists","Wolters Kluwer Health","Baishideng Publishing Group","Federation of American Societies for Experimental Biology (FASEB)","American Chemical Society (ACS)","Royal Society of Chemistry (RSC)","EMBO press","Genetics Society of America","American Association for the Advancement of Science (AAAS)","Bio-Protocol"],"ids":[8,17,202,208,19,11,6909,18,344,326,162,6627,6941,801,39,1097,31,7871,695,872,32,7719,3243,40,123,9415,3356,189,7047],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/jeWDR7NTA9Ab5MVz8Uqjl2RPCndzFjSyl0FaRAHg_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/doUaFUZdxUEQjLi1TwZjGHi8HXYNWWSk04dSC6Xh_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/ruydfaB80LDjlkYqsfOeUAZohOIODyq7bQzis5O7_medium.webp","\/storage\/images\/resized\/Teo7jjEEA71rw8mjlFC8lDbYotZhhtZbL0J9gPs2_medium.webp","\/storage\/images\/resized\/s6JtNKPXrzklKtbQZ4HrtzuJT0P7eHPX9U8x0nss_medium.webp","\/storage\/images\/resized\/ko5EaisKGm66J1hHXtfEgVEeeWb9uOcMofpNuf0a_medium.webp","\/storage\/images\/resized\/6QE8LXWrLpkoy5A2te6hw7who46GeCoTYIstuoAz_medium.webp","\/storage\/images\/resized\/bKOjgSt9mSGUD9fzKZyqcKSIgDCDSMd1ZWjJOZzx_medium.webp","\/storage\/images\/resized\/dfTCxFYwjmdhSAwAcIOV9PfBdAN17zTMvybxH2kG_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/9EVpRifnnUJt6oHGY5GfsfSXTEz5wzbdgnZqiF5m_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp",""],"datasets":[{"label":"","data":[110,82,47,35,31,31,17,16,7,6,6,6,6,5,3,3,3,2,2,2,2,2,2,2,2,2,2,2,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[20.41,15.21,8.72,6.49,5.75,5.75,3.15,2.97,1.3,1.11,1.11,1.11,1.11,0.93,0.56,0.56,0.56,0.37,0.37,0.37,0.37,0.37,0.37,0.37,0.37,0.37,0.37,0.37,0.37],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Metrics

Found error?

Publisher

—