volume 22, issue 1, publication number 170
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
Park Soo-Ji
1, 2
,
Jeong Tae Yeong
1, 2
,
Shin Seung Kyun
2
,
Yoon Da-Eun
1, 2
,
Lim Soo Yeon
3
,
Kim Sol Pin
3
,
Choi Jungmin
1
,
Lee Hyunji
4
,
Hong Jeong-Im
2
,
Ahn Jinhee
2
,
Seong Je Kyung
3, 5, 6
,
Kim Kyoungmi
1, 2
1
Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea
|
2
Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea
|
3
Korea Mouse Phenotyping Center, Seoul National University, Seoul, Republic of Korea
|
4
Center for Genome Engineering, Institute for Basic Science, Daejeon, Republic of Korea
|
5
Interdisciplinary Program for Bioinformatics, Program for Cancer Biology, BIO-MAX/N-Bio Institute, Seoul National University, Seoul, Republic of Korea
|
6
Laboratory of Developmental Biology and Genomics, BK21 Program Plus for Advanced Veterinary Science, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
|
Publication type: Journal Article
Publication date: 2021-06-03
—
PubMed ID:
34082781
Abstract
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.
Citations by journals
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1
2
3
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
3 publications, 4.84%
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Cells
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Cells
3 publications, 4.84%
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Nature Communications
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Nature Communications
3 publications, 4.84%
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Nature Biotechnology
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Nature Biotechnology
3 publications, 4.84%
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Nucleic Acids Research
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Nucleic Acids Research
3 publications, 4.84%
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The CRISPR Journal
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The CRISPR Journal
2 publications, 3.23%
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Molecular Cell
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Molecular Cell
2 publications, 3.23%
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Experimental and Molecular Medicine
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Experimental and Molecular Medicine
2 publications, 3.23%
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Military Medical Research
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Military Medical Research
1 publication, 1.61%
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Frontiers in Genome Editing
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Frontiers in Genome Editing
1 publication, 1.61%
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Frontiers in Cell and Developmental Biology
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Frontiers in Cell and Developmental Biology
1 publication, 1.61%
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Frontiers in Immunology
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Frontiers in Immunology
1 publication, 1.61%
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Molecular Cancer
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Molecular Cancer
1 publication, 1.61%
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Transgenic Research
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Transgenic Research
1 publication, 1.61%
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Nature Reviews Genetics
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Nature Reviews Genetics
1 publication, 1.61%
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Nature Protocols
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Nature Protocols
1 publication, 1.61%
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Trends in Biotechnology
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Trends in Biotechnology
1 publication, 1.61%
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Computational and Structural Biotechnology Journal
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Computational and Structural Biotechnology Journal
1 publication, 1.61%
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Journal of Hepatology
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Journal of Hepatology
1 publication, 1.61%
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Seminars in Cell and Developmental Biology
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Seminars in Cell and Developmental Biology
1 publication, 1.61%
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Nature Biomedical Engineering
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Nature Biomedical Engineering
1 publication, 1.61%
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Molecular Therapy
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Molecular Therapy
1 publication, 1.61%
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Cell
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Cell
1 publication, 1.61%
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Endocrinology Diabetes & Metabolism
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Endocrinology Diabetes & Metabolism
1 publication, 1.61%
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Molecular Therapy - Nucleic Acids
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Molecular Therapy - Nucleic Acids
1 publication, 1.61%
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Plant Physiology
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Plant Physiology
1 publication, 1.61%
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Synthetic and Systems Biotechnology
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Synthetic and Systems Biotechnology
1 publication, 1.61%
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Journal of Biological Chemistry
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Journal of Biological Chemistry
1 publication, 1.61%
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Engineering Microbiology
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Engineering Microbiology
1 publication, 1.61%
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1
2
3
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Citations by publishers
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2
4
6
8
10
12
14
16
18
20
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Springer Nature
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Springer Nature
19 publications, 30.65%
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Elsevier
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Elsevier
14 publications, 22.58%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 11.29%
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Frontiers Media S.A.
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Frontiers Media S.A.
4 publications, 6.45%
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Oxford University Press
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Oxford University Press
3 publications, 4.84%
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Mary Ann Liebert
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Mary Ann Liebert
2 publications, 3.23%
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Wiley
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Wiley
2 publications, 3.23%
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American Society of Plant Biologists
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American Society of Plant Biologists
1 publication, 1.61%
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KeAi Communications Co.
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KeAi Communications Co.
1 publication, 1.61%
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American Society for Biochemistry and Molecular Biology
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American Society for Biochemistry and Molecular Biology
1 publication, 1.61%
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Higher Education Press
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Higher Education Press
1 publication, 1.61%
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Taylor & Francis
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Taylor & Francis
1 publication, 1.61%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 1.61%
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2
4
6
8
10
12
14
16
18
20
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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