volume 37 issue 8 pages 2179-2182

Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia.

Masatoshi YAMAZAKI
Shigeru Tansho
Jun KISUGI
Koji Muramoto
Hisao KAMIYA
Publication typeJournal Article
Publication date2011-12-08
scimago Q3
wos Q3
SJR0.349
CiteScore2.7
Impact factor1.3
ISSN00092363, 13475223
PubMed ID:  2598320
General Chemistry
Drug Discovery
General Medicine
Abstract
A novel cytolytic factor, dolabellanin P, was purified to apparent homogeneity from the purple fluid of the sea hare, Dolabella auricularia. Purified dolabellanin P is a single polypeptide of 60 kDa. The amino acid composition and the N-terminus of the factor were also determined. This factor nonspecifically lysed all the cells tested at 50-200 ng protein/ml. Dolabellanin P caused complete cytolysis within 2 h. The factor is distinct from antineoplastic glycoproteins previously isolated from eggs (aplysianin E) or albumen gland (aplysianin A) of Aplysia kurodai in terms of certain cytolytic properties. These results suggest that dolabellanin P, found in the sea hare, a marine invertebrate, is a new cytolytic factor.
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GOST Copy
YAMAZAKI M. et al. Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia. // Chemical and Pharmaceutical Bulletin. 2011. Vol. 37. No. 8. pp. 2179-2182.
GOST all authors (up to 50) Copy
YAMAZAKI M., Tansho S., KISUGI J., Muramoto K., KAMIYA H. Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia. // Chemical and Pharmaceutical Bulletin. 2011. Vol. 37. No. 8. pp. 2179-2182.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1248/cpb.37.2179
UR - https://doi.org/10.1248/cpb.37.2179
TI - Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia.
T2 - Chemical and Pharmaceutical Bulletin
AU - YAMAZAKI, Masatoshi
AU - Tansho, Shigeru
AU - KISUGI, Jun
AU - Muramoto, Koji
AU - KAMIYA, Hisao
PY - 2011
DA - 2011/12/08
PB - Pharmaceutical Society of Japan
SP - 2179-2182
IS - 8
VL - 37
PMID - 2598320
SN - 0009-2363
SN - 1347-5223
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2011_YAMAZAKI,
author = {Masatoshi YAMAZAKI and Shigeru Tansho and Jun KISUGI and Koji Muramoto and Hisao KAMIYA},
title = {Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia.},
journal = {Chemical and Pharmaceutical Bulletin},
year = {2011},
volume = {37},
publisher = {Pharmaceutical Society of Japan},
month = {dec},
url = {https://doi.org/10.1248/cpb.37.2179},
number = {8},
pages = {2179--2182},
doi = {10.1248/cpb.37.2179}
}
MLA
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MLA Copy
YAMAZAKI, Masatoshi, et al. “Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia..” Chemical and Pharmaceutical Bulletin, vol. 37, no. 8, Dec. 2011, pp. 2179-2182. https://doi.org/10.1248/cpb.37.2179.