Open Access
RNA, volume 19, issue 6, pages 841-851
RNA recognition by the DNA end-binding Ku heterodimer
1
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado BioFrontiers Institute, Boulder, Colorado 80309-0596, USA
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Publication type: Journal Article
Publication date: 2013-04-22
PubMed ID:
23610127
Molecular Biology
Abstract
Most nucleic acid-binding proteins selectively bind either DNA or RNA, but not both nucleic acids. The Saccharomyces cerevisiae Ku heterodimer is unusual in that it has two very different biologically relevant binding modes: (1) Ku is a sequence-nonspecific double-stranded DNA end-binding protein with prominent roles in nonhomologous end-joining and telomeric capping, and (2) Ku associates with a specific stem-loop of TLC1, the RNA subunit of budding yeast telomerase, and is necessary for proper nuclear localization of this ribonucleoprotein enzyme. TLC1 RNA-binding and dsDNA-binding are mutually exclusive, so they may be mediated by the same site on Ku. Although dsDNA binding by Ku is well studied, much less is known about what features of an RNA hairpin enable specific recognition by Ku. To address this question, we localized the Ku-binding site of the TLC1 hairpin with single-nucleotide resolution using phosphorothioate footprinting, used chemical modification to identify an unpredicted motif within the hairpin secondary structure, and carried out mutagenesis of the stem-loop to ascertain the critical elements within the RNA that permit Ku binding. Finally, we provide evidence that the Ku-binding site is present in additional budding yeast telomerase RNAs and discuss the possibility that RNA binding is a conserved function of the Ku heterodimer.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Dalby A. R. et al. RNA recognition by the DNA end-binding Ku heterodimer // RNA. 2013. Vol. 19. No. 6. pp. 841-851.
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Dalby A. R., Goodrich K. J., Pfingsten J. S., Cech T. R. RNA recognition by the DNA end-binding Ku heterodimer // RNA. 2013. Vol. 19. No. 6. pp. 841-851.
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TY - JOUR
DO - 10.1261/rna.038703.113
UR - https://doi.org/10.1261%2Frna.038703.113
TI - RNA recognition by the DNA end-binding Ku heterodimer
T2 - RNA
AU - Dalby, Andrew R.
AU - Goodrich, Karen J.
AU - Pfingsten, Jennifer S
AU - Cech, Thomas R.
PY - 2013
DA - 2013/04/22 00:00:00
PB - Cold Spring Harbor Laboratory
SP - 841-851
IS - 6
VL - 19
PMID - 23610127
SN - 1355-8382
SN - 1469-9001
ER -
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@article{2013_Dalby,
author = {Andrew R. Dalby and Karen J. Goodrich and Jennifer S Pfingsten and Thomas R. Cech},
title = {RNA recognition by the DNA end-binding Ku heterodimer},
journal = {RNA},
year = {2013},
volume = {19},
publisher = {Cold Spring Harbor Laboratory},
month = {apr},
url = {https://doi.org/10.1261%2Frna.038703.113},
number = {6},
pages = {841--851},
doi = {10.1261/rna.038703.113}
}
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MLA
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Dalby, Andrew R., et al. “RNA recognition by the DNA end-binding Ku heterodimer.” RNA, vol. 19, no. 6, Apr. 2013, pp. 841-851. https://doi.org/10.1261%2Frna.038703.113.