Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow.
Detection and extraction of circulating tumor cells and other rare objects in the bloodstream are of great interest for modern diagnostics, but devices that can solve this problem for the whole blood volume of laboratory animals are still rare. Here we have developed SPIM-based lightsheet flow cytometer for the detection of fluorescently-labeled objects in whole blood. The bypass channel between two blood vessels connected with the external flow cell was used to visualize, detect, and magnetically separate fluorescently-labeled objects without hydrodynamic focusing. Carriers for targeted drug delivery were used as model objects to test the device performance. They were injected into the bloodstream of the rat, detected fluorescently, and then captured from the bloodstream by a magnetic separator prior to filtration in organs. Carriers extracted from the whole blood were studied by a number of in vitro methods.
Citations by journals
1
2
|
|
Pharmaceutics
|
Pharmaceutics
2 publications, 22.22%
|
Biomaterials Science
|
Biomaterials Science
1 publication, 11.11%
|
Biomedical Optics Express
|
Biomedical Optics Express
1 publication, 11.11%
|
Molecules
|
Molecules
1 publication, 11.11%
|
PLoS ONE
|
PLoS ONE
1 publication, 11.11%
|
Cytometry. Part A : the journal of the International Society for Analytical Cytology
|
Cytometry. Part A : the journal of the International Society for Analytical Cytology
1 publication, 11.11%
|
1
2
|
Citations by publishers
1
2
3
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 33.33%
|
Royal Society of Chemistry (RSC)
|
Royal Society of Chemistry (RSC)
1 publication, 11.11%
|
Optical Society of America
|
Optical Society of America
1 publication, 11.11%
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
1 publication, 11.11%
|
Wiley
|
Wiley
1 publication, 11.11%
|
1
2
3
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.