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Biomedical Optics Express, volume 12, issue 1, pages 380

Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow.

Verkhovskii Roman A 1
Kozlova Anastasiia A 1
Sindeeva Olga A 1
Kozhevnikov Ilya O 2
Grishin Oleg V 1
Makarkin Mikhail A 1
Ermakov Alexey V 1
Publication typeJournal Article
Publication date2020-12-15
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor3.4
ISSN21567085
PubMed ID:  33659080
Biotechnology
Atomic and Molecular Physics, and Optics
Abstract

Detection and extraction of circulating tumor cells and other rare objects in the bloodstream are of great interest for modern diagnostics, but devices that can solve this problem for the whole blood volume of laboratory animals are still rare. Here we have developed SPIM-based lightsheet flow cytometer for the detection of fluorescently-labeled objects in whole blood. The bypass channel between two blood vessels connected with the external flow cell was used to visualize, detect, and magnetically separate fluorescently-labeled objects without hydrodynamic focusing. Carriers for targeted drug delivery were used as model objects to test the device performance. They were injected into the bloodstream of the rat, detected fluorescently, and then captured from the bloodstream by a magnetic separator prior to filtration in organs. Carriers extracted from the whole blood were studied by a number of in vitro methods.

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Verkhovskii R. A. et al. Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow. // Biomedical Optics Express. 2020. Vol. 12. No. 1. p. 380.
GOST all authors (up to 50) Copy
Verkhovskii R. A., Kozlova A. A., Sindeeva O. A., Kozhevnikov I. O., Prikhozhdenko E. S., Mayorova O. A., Grishin O. V., Makarkin M. A., Ermakov A. V., Abdurashitov A. S., Tuchin V. V., Bratashov D. Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow. // Biomedical Optics Express. 2020. Vol. 12. No. 1. p. 380.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1364/BOE.413845
UR - https://doi.org/10.1364%2FBOE.413845
TI - Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow.
T2 - Biomedical Optics Express
AU - Kozlova, Anastasiia A
AU - Sindeeva, Olga A
AU - Kozhevnikov, Ilya O
AU - Prikhozhdenko, Ekaterina S
AU - Mayorova, Oksana A.
AU - Grishin, Oleg V
AU - Makarkin, Mikhail A
AU - Abdurashitov, Arkady S.
AU - Tuchin, Valery V.
AU - Bratashov, Daniil
AU - Verkhovskii, Roman A
AU - Ermakov, Alexey V
PY - 2020
DA - 2020/12/15 00:00:00
PB - Optical Society of America
SP - 380
IS - 1
VL - 12
PMID - 33659080
SN - 2156-7085
ER -
BibTex |
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BibTex Copy
@article{2020_Verkhovskii
author = {Anastasiia A Kozlova and Olga A Sindeeva and Ilya O Kozhevnikov and Ekaterina S Prikhozhdenko and Oksana A. Mayorova and Oleg V Grishin and Mikhail A Makarkin and Arkady S. Abdurashitov and Valery V. Tuchin and Daniil Bratashov and Roman A Verkhovskii and Alexey V Ermakov},
title = {Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow.},
journal = {Biomedical Optics Express},
year = {2020},
volume = {12},
publisher = {Optical Society of America},
month = {dec},
url = {https://doi.org/10.1364%2FBOE.413845},
number = {1},
pages = {380},
doi = {10.1364/BOE.413845}
}
MLA
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MLA Copy
Verkhovskii, Roman A., et al. “Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow..” Biomedical Optics Express, vol. 12, no. 1, Dec. 2020, p. 380. https://doi.org/10.1364%2FBOE.413845.
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