Open Access
Biomedical Optics Express, volume 13, issue 2, pages 633
Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma
Zherebtsov Evgeny A.
1, 2
,
Potapova Elena V
1
,
Mamoshin Andrian V
1, 3
,
Dremin Viktor
1, 4
,
Abramov Andrey Y
1, 5
,
3
Orel Regional Clinical Hospital
5
UCL Queen Square Institute of Neurology
Publication type: Journal Article
Publication date: 2022-01-06
Journal:
Biomedical Optics Express
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 3.4
ISSN: 21567085
PubMed ID:
35284175
Biotechnology
Atomic and Molecular Physics, and Optics
Abstract
This work presents results of in vivo and in situ measurements of hepatocellular carcinoma by a developed optical biopsy system. Here, we describe the technical details of the implementation of fluorescence lifetime and diffuse reflectance measurements by the system, equipped with an original needle optical probe, compatible with the 17.5G biopsy needle standard. The fluorescence lifetime measurements observed by the setup were verified in fresh solutions of NADH and FAD++, and then applied in a murine model for the characterisation of inoculated hepatocellular carcinoma (HCC) and adjacent liver tissue. The technique, applied in vivo and in situ and supplemented by measurements of blood oxygen saturation, made it possible to reveal statistically significant transformation in the set of measured parameters linked with the cellular pools of NADH and NADPH. In the animal model, we demonstrate that the characteristic changes in registered fluorescent parameters can be used to reliably distinguish the HCC tissue, liver tissue in the control, and the metabolically changed liver tissues of animals with the developed HCC tumour. For further transition to clinical applications, the optical biopsy system was tested during the routing procedure of the PNB in humans with suspected HCC. The comparison of the data from murine and human HCC tissues suggests that the tested animal model is generally representative in the sense of the registered fluorescence lifetime parameters, while statistically significant differences between their absolute values can still be observed.
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Zherebtsov E. A. et al. Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma // Biomedical Optics Express. 2022. Vol. 13. No. 2. p. 633.
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Zherebtsov E. A., Potapova E. V., Mamoshin A. V., Shupletsov V. V., Kandurova K. Y., Dremin V., Abramov A. Y., Dunaev A. V. Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma // Biomedical Optics Express. 2022. Vol. 13. No. 2. p. 633.
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TY - JOUR
DO - 10.1364/BOE.447687
UR - https://doi.org/10.1364%2FBOE.447687
TI - Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma
T2 - Biomedical Optics Express
AU - Zherebtsov, Evgeny A.
AU - Potapova, Elena V
AU - Dremin, Viktor
AU - Abramov, Andrey Y
AU - Dunaev, Andrey V.
AU - Mamoshin, Andrian V
AU - Shupletsov, Valery V
AU - Kandurova, Ksenia Y
PY - 2022
DA - 2022/01/06 00:00:00
PB - Optical Society of America
SP - 633
IS - 2
VL - 13
PMID - 35284175
SN - 2156-7085
ER -
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@article{2022_Zherebtsov,
author = {Evgeny A. Zherebtsov and Elena V Potapova and Viktor Dremin and Andrey Y Abramov and Andrey V. Dunaev and Andrian V Mamoshin and Valery V Shupletsov and Ksenia Y Kandurova},
title = {Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma},
journal = {Biomedical Optics Express},
year = {2022},
volume = {13},
publisher = {Optical Society of America},
month = {jan},
url = {https://doi.org/10.1364%2FBOE.447687},
number = {2},
pages = {633},
doi = {10.1364/BOE.447687}
}
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MLA
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Zherebtsov, Evgeny A., et al. “Fluorescence lifetime needle optical biopsy discriminates hepatocellular carcinoma.” Biomedical Optics Express, vol. 13, no. 2, Jan. 2022, p. 633. https://doi.org/10.1364%2FBOE.447687.