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PLoS Genetics, volume 13, issue 11, pages e1007092

Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway

Hanson Sara J 1
Byrne Kevin P 2
Wolfe Kenneth A. 2
1
 
UCD Conway Institute, School of Medicine, University College Dublin, Dublin 4, Ireland,
2
 
UCD Conway Institute, School of Medicine, University College Dublin, Dublin 4, Ireland
Publication typeJournal Article
Publication date2017-11-27
Journal: PLoS Genetics
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor4.5
ISSN15537390, 15537404
Cancer Research
Molecular Biology
Genetics
Ecology, Evolution, Behavior and Systematics
Genetics (clinical)
Abstract
In haploid cells of Ogataea (Hansenula) polymorpha an environmental signal, nitrogen starvation, induces a reversible change in the structure of a chromosome. This process, mating-type switching, inverts a 19-kb DNA region to place either MATa or MATα genes under centromeric repression of transcription, depending on the orientation of the region. Here, we investigated the genetic pathway that controls switching. We characterized the transcriptomes of haploid and diploid O. polymorpha by RNAseq in rich and nitrogen-deficient media, and found that there are no constitutively a-specific or α-specific genes other than the MAT genes themselves. We mapped a switching defect in a sibling species (O. parapolymorpha strain DL-1) by interspecies bulk segregant analysis to a frameshift in the transcription factor EFG1, which in Candida albicans regulates filamentous growth and white-opaque switching. Gene knockout, overexpression and ChIPseq experiments show that EFG1 regulates RME1, which in turn regulates STE12, to achieve mating-type switching. All three genes are necessary both for switching and for mating. Overexpression of RME1 or STE12 is sufficient to induce switching without a nitrogen depletion signal. The homologous recombination genes RAD51 and RAD17 are also necessary for switching. The pathway controlling switching in O. polymorpha shares no components with the regulation of HO in S. cerevisiae, which does not involve any environmental signal, but it shares some components with mating-type switching in Kluyveromyces lactis and with white-opaque phenotypic switching in C. albicans.

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Hanson S. J., Byrne K. P., Wolfe K. A. Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway // PLoS Genetics. 2017. Vol. 13. No. 11. p. e1007092.
GOST all authors (up to 50) Copy
Hanson S. J., Byrne K. P., Wolfe K. A. Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway // PLoS Genetics. 2017. Vol. 13. No. 11. p. e1007092.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1371/journal.pgen.1007092
UR - https://doi.org/10.1371%2Fjournal.pgen.1007092
TI - Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway
T2 - PLoS Genetics
AU - Hanson, Sara J
AU - Byrne, Kevin P
AU - Wolfe, Kenneth A.
PY - 2017
DA - 2017/11/27 00:00:00
PB - Public Library of Science (PLoS)
SP - e1007092
IS - 11
VL - 13
PMID - 29176810
SN - 1553-7390
SN - 1553-7404
ER -
BibTex |
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BibTex Copy
@article{2017_Hanson
author = {Sara J Hanson and Kevin P Byrne and Kenneth A. Wolfe},
title = {Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway},
journal = {PLoS Genetics},
year = {2017},
volume = {13},
publisher = {Public Library of Science (PLoS)},
month = {nov},
url = {https://doi.org/10.1371%2Fjournal.pgen.1007092},
number = {11},
pages = {e1007092},
doi = {10.1371/journal.pgen.1007092}
}
MLA
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MLA Copy
Hanson, Sara J., et al. “Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway.” PLoS Genetics, vol. 13, no. 11, Nov. 2017, p. e1007092. https://doi.org/10.1371%2Fjournal.pgen.1007092.
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