Open Access

PLoS ONE, volume 7, issue 12, pages e52020

Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging

Tang Xinyan ¹

Jing Liufang ¹

Chen Jun ²

Hide authors affiliations Show authors affiliations: 2 affiliations

Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America |

Department of Orthopedic Surgery, Duke University Medical Center, Durham, North Carolina, United States of America |

Publication type: Journal Article

Publication date: 2012-12-19

Public Library of Science (PLoS)

Journal: PLoS ONE

Quartile SCImago

Quartile WOS

Impact factor: 3.7

ISSN: 19326203

DOI: 10.1371/journal.pone.0052020

Copy DOI

PubMed ID: 23284858

Multidisciplinary

Abstract

Intervertebral disc (IVD) disorder and age-related degeneration are believed to contribute to low back pain. Cell-based therapies represent a promising strategy to treat disc degeneration; however, the cellular and molecular characteristics of disc cells during IVD maturation and aging still remain poorly defined. This study investigated novel molecular markers and their age-related changes in the rat IVD. Affymetrix cDNA microarray analysis was conducted to identify a new set of genes characterizing immature nucleus pulposus (NP) cells. Among these markers, select neuronal-related proteins (Basp1, Ncdn and Nrp-1), transcriptional factor (Brachyury T), and cell surface receptors (CD24, CD90, CD155 and CD221) were confirmed by real-time PCR and immunohistochemical (IHC) staining for differential expression between IVD tissue regions and among various ages (1, 12 and 21 months). NP cells generally possessed higher levels of mRNA or protein expression for all aforementioned markers, with the exception of CD90 in anulus fibrosus (AF) cells. In addition, CD protein (CD24 and CD90) and Brachyury (T) expression in immature disc cells were also confirmed via flow cytometry. Similar to IHC staining, results revealed a higher percentage of immature NP cells expressing CD24 and Brachyury, while higher percentage of immature AF cells was stained positively for CD90. Altogether, this study identifies that tissue-specific gene expression and age-related differential expression of the above markers do exist in immature and aged disc cells. These age-related phenotype changes provide a new insight for a molecular profile that may be used to characterize NP cells for developing cell-based regenerative therapy for IVD regeneration.

By date By citations

Citations by journals

	1 2 3 4 5 6 7
JOR Spine	JOR Spine, 7, 9.09% JOR Spine 7 publications, 9.09%
Journal of Orthopaedic Research	Journal of Orthopaedic Research, 5, 6.49% Journal of Orthopaedic Research 5 publications, 6.49%
Scientific Reports	Scientific Reports, 4, 5.19% Scientific Reports 4 publications, 5.19%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 3, 3.9% International Journal of Molecular Sciences 3 publications, 3.9%
Biomaterials	Biomaterials, 3, 3.9% Biomaterials 3 publications, 3.9%
PLoS ONE	PLoS ONE, 3, 3.9% PLoS ONE 3 publications, 3.9%
Tissue Engineering - Part A.	Tissue Engineering - Part A., 2, 2.6% Tissue Engineering - Part A. 2 publications, 2.6%
Spine	Spine, 2, 2.6% Spine 2 publications, 2.6%
Nature Communications	Nature Communications, 2, 2.6% Nature Communications 2 publications, 2.6%
Matrix Biology	Matrix Biology, 2, 2.6% Matrix Biology 2 publications, 2.6%
Stem Cells International	Stem Cells International, 2, 2.6% Stem Cells International 2 publications, 2.6%
Molecular Biology	Molecular Biology, 1, 1.3% Molecular Biology 1 publication, 1.3%
Journal of Biomechanical Engineering	Journal of Biomechanical Engineering, 1, 1.3% Journal of Biomechanical Engineering 1 publication, 1.3%
Medecine/Sciences	Medecine/Sciences, 1, 1.3% Medecine/Sciences 1 publication, 1.3%
Epigenomics	Epigenomics, 1, 1.3% Epigenomics 1 publication, 1.3%
International Journal of Molecular Medicine	International Journal of Molecular Medicine, 1, 1.3% International Journal of Molecular Medicine 1 publication, 1.3%
Molecular Medicine Reports	Molecular Medicine Reports, 1, 1.3% Molecular Medicine Reports 1 publication, 1.3%
American Journal of Physical Medicine and Rehabilitation	American Journal of Physical Medicine and Rehabilitation, 1, 1.3% American Journal of Physical Medicine and Rehabilitation 1 publication, 1.3%
Global Spine Journal	Global Spine Journal, 1, 1.3% Global Spine Journal 1 publication, 1.3%
Journal of Developmental Biology	Journal of Developmental Biology, 1, 1.3% Journal of Developmental Biology 1 publication, 1.3%
Applied Sciences (Switzerland)	Applied Sciences (Switzerland), 1, 1.3% Applied Sciences (Switzerland) 1 publication, 1.3%
Cytotechnology	Cytotechnology, 1, 1.3% Cytotechnology 1 publication, 1.3%
European Spine Journal	European Spine Journal, 1, 1.3% European Spine Journal 1 publication, 1.3%
Archives of Orthopaedic and Trauma Surgery	Archives of Orthopaedic and Trauma Surgery, 1, 1.3% Archives of Orthopaedic and Trauma Surgery 1 publication, 1.3%
Cellular and Molecular Bioengineering	Cellular and Molecular Bioengineering, 1, 1.3% Cellular and Molecular Bioengineering 1 publication, 1.3%
Stem Cell Research and Therapy	Stem Cell Research and Therapy, 1, 1.3% Stem Cell Research and Therapy 1 publication, 1.3%
Journal of Biomechanics	Journal of Biomechanics, 1, 1.3% Journal of Biomechanics 1 publication, 1.3%
Seminars in Cell and Developmental Biology	Seminars in Cell and Developmental Biology, 1, 1.3% Seminars in Cell and Developmental Biology 1 publication, 1.3%
Biochemical and Biophysical Research Communications	Biochemical and Biophysical Research Communications, 1, 1.3% Biochemical and Biophysical Research Communications 1 publication, 1.3%
	1 2 3 4 5 6 7

Citations by publishers

	2 4 6 8 10 12 14 16
Elsevier	Elsevier, 15, 19.48% Elsevier 15 publications, 19.48%
Wiley	Wiley, 15, 19.48% Wiley 15 publications, 19.48%
Springer Nature	Springer Nature, 11, 14.29% Springer Nature 11 publications, 14.29%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 7, 9.09% Multidisciplinary Digital Publishing Institute (MDPI) 7 publications, 9.09%
Wolters Kluwer Health	Wolters Kluwer Health, 3, 3.9% Wolters Kluwer Health 3 publications, 3.9%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 3, 3.9% Public Library of Science (PLoS) 3 publications, 3.9%
Hindawi Limited	Hindawi Limited, 3, 3.9% Hindawi Limited 3 publications, 3.9%
Mary Ann Liebert	Mary Ann Liebert, 2, 2.6% Mary Ann Liebert 2 publications, 2.6%
Spandidos Publications	Spandidos Publications, 2, 2.6% Spandidos Publications 2 publications, 2.6%
Pleiades Publishing	Pleiades Publishing, 1, 1.3% Pleiades Publishing 1 publication, 1.3%
ASME	ASME, 1, 1.3% ASME 1 publication, 1.3%
Editions E D K	Editions E D K, 1, 1.3% Editions E D K 1 publication, 1.3%
Future Medicine	Future Medicine, 1, 1.3% Future Medicine 1 publication, 1.3%
SAGE	SAGE, 1, 1.3% SAGE 1 publication, 1.3%
Taylor & Francis	Taylor & Francis, 1, 1.3% Taylor & Francis 1 publication, 1.3%
British Editorial Society of Bone and Joint Surgery	British Editorial Society of Bone and Joint Surgery, 1, 1.3% British Editorial Society of Bone and Joint Surgery 1 publication, 1.3%
Oxford University Press	Oxford University Press, 1, 1.3% Oxford University Press 1 publication, 1.3%
BMJ	BMJ, 1, 1.3% BMJ 1 publication, 1.3%
Korean Spinal Neurosurgery Society	Korean Spinal Neurosurgery Society, 1, 1.3% Korean Spinal Neurosurgery Society 1 publication, 1.3%
Japanese Society for Spine Surgery and Related Research	Japanese Society for Spine Surgery and Related Research, 1, 1.3% Japanese Society for Spine Surgery and Related Research 1 publication, 1.3%
Portland Press	Portland Press, 1, 1.3% Portland Press 1 publication, 1.3%
	2 4 6 8 10 12 14 16

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2013,2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[5,7,6,6,5,11,4,7,6,6,10,4],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["6.49","9.09","7.79","7.79","6.49","14.29","5.19","9.09","7.79","7.79","12.99","5.19"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["JOR Spine","Journal of Orthopaedic Research","Scientific Reports","International Journal of Molecular Sciences","Biomaterials","PLoS ONE","Tissue Engineering - Part A.","Spine","Nature Communications","Matrix Biology","Stem Cells International","Molecular Biology","Journal of Biomechanical Engineering","Medecine\/Sciences","Epigenomics","International Journal of Molecular Medicine","Molecular Medicine Reports","American Journal of Physical Medicine and Rehabilitation","Global Spine Journal","Journal of Developmental Biology","Applied Sciences (Switzerland)","Cytotechnology","European Spine Journal","Archives of Orthopaedic and Trauma Surgery","Cellular and Molecular Bioengineering","Stem Cell Research and Therapy","Journal of Biomechanics","Seminars in Cell and Developmental Biology","Biochemical and Biophysical Research Communications"],"ids":[27195,22248,13767,14627,13732,24963,3218,283,3231,6437,22095,1134,24181,3775,12707,16008,4737,1141,23877,8406,16650,20706,10450,2240,2588,7883,18800,9325,12390],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/Zy5YST233rzRbZP24I24MzZm64hz20ZV9qQopmMn_medium.webp","\/storage\/images\/resized\/6QE8LXWrLpkoy5A2te6hw7who46GeCoTYIstuoAz_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/hqfzhQAjTGlNSRs6yzFNITgjSMm9Jr2QuotJHIvE_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/yKbGpvfNleUTiwBfKt4XcIFlZMo3HgWv1KKVqn4g_medium.webp","","\/storage\/images\/resized\/AN2jnDN59NmKaS4CWLHAGstADL2DbnatQScsFA6C_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/6QE8LXWrLpkoy5A2te6hw7who46GeCoTYIstuoAz_medium.webp","\/storage\/images\/resized\/ruydfaB80LDjlkYqsfOeUAZohOIODyq7bQzis5O7_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[7,5,4,3,3,3,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[9.09,6.49,5.19,3.9,3.9,3.9,2.6,2.6,2.6,2.6,2.6,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Wiley","Springer Nature","Multidisciplinary Digital Publishing Institute (MDPI)","Wolters Kluwer Health","Public Library of Science (PLoS)","Hindawi Limited","Mary Ann Liebert","Spandidos Publications","Pleiades Publishing","ASME","Editions E D K","Future Medicine","SAGE","Taylor & Francis","British Editorial Society of Bone and Joint Surgery","Oxford University Press","BMJ","Korean Spinal Neurosurgery Society","Japanese Society for Spine Surgery and Related Research","Portland Press"],"ids":[17,11,8,202,32,344,6921,230,1097,101,6977,1381,6949,31,18,1169,19,6954,4701,6099,801],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/6QE8LXWrLpkoy5A2te6hw7who46GeCoTYIstuoAz_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/hqfzhQAjTGlNSRs6yzFNITgjSMm9Jr2QuotJHIvE_medium.webp","\/storage\/images\/resized\/Zy5YST233rzRbZP24I24MzZm64hz20ZV9qQopmMn_medium.webp","\/storage\/images\/resized\/8zKfOwdd5XirYzTQfqwp7U8IEQc8ckIOjyYQSBkk_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/yKbGpvfNleUTiwBfKt4XcIFlZMo3HgWv1KKVqn4g_medium.webp","","\/storage\/images\/resized\/AN2jnDN59NmKaS4CWLHAGstADL2DbnatQScsFA6C_medium.webp","\/storage\/images\/resized\/ruydfaB80LDjlkYqsfOeUAZohOIODyq7bQzis5O7_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/hysFKdIDQMKHPbF1uLxJ3M6dQA4gPVuC6ZeA7m5a_medium.webp","","","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp"],"datasets":[{"label":"","data":[15,15,11,7,3,3,3,2,2,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[19.48,19.48,14.29,9.09,3.9,3.9,3.9,2.6,2.6,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3,1.3],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Publication PDF

Metrics

Cite this

GOST |

Cite this

GOST Copy

Tang X., Jing L., Chen J. Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging // PLoS ONE. 2012. Vol. 7. No. 12. p. e52020.

GOST all authors (up to 50) Copy

Tang X., Jing L., Chen J. Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging // PLoS ONE. 2012. Vol. 7. No. 12. p. e52020.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1371/journal.pone.0052020

UR - https://doi.org/10.1371%2Fjournal.pone.0052020

TI - Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging

T2 - PLoS ONE

AU - Tang, Xinyan

AU - Jing, Liufang

AU - Chen, Jun

PY - 2012

DA - 2012/12/19 00:00:00

PB - Public Library of Science (PLoS)

SP - e52020

IS - 12

VL - 7

PMID - 23284858

SN - 1932-6203

ER -

BibTex |

Cite this

BibTex Copy

@article{2012_Tang,

author = {Xinyan Tang and Liufang Jing and Jun Chen},

title = {Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging},

journal = {PLoS ONE},

year = {2012},

volume = {7},

publisher = {Public Library of Science (PLoS)},

month = {dec},

url = {https://doi.org/10.1371%2Fjournal.pone.0052020},

number = {12},

pages = {e52020},

doi = {10.1371/journal.pone.0052020}

}

MLA

Cite this

MLA Copy

Tang, Xinyan, et al. “Changes in the Molecular Phenotype of Nucleus Pulposus Cells with Intervertebral Disc Aging.” PLoS ONE, vol. 7, no. 12, Dec. 2012, p. e52020. https://doi.org/10.1371%2Fjournal.pone.0052020.

Found error?

Publisher

Public Library of Science (PLoS)

Journal

PLoS ONE

Quartile SCImago

Quartile WOS

Impact factor

3.7

ISSN

19326203 (Print)