, volume 387 , issue 3 , pages 257-261

Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation

Anna A Neschastnova ¹

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Institute of Carcinogenesis, Blokhin Cancer Research Centre, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow 115478, Russia. |

Publication type: Journal Article

Publication date: 2006-01-01

Walter de Gruyter

Biological Chemistry

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wos Q3

SJR: 1.117

CiteScore: 7.0

Impact factor: 2.4

ISSN: 14316730, 14374315

DOI: 10.1515/BC.2006.034

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PubMed ID: 16542146

Biochemistry

Molecular Biology

Clinical Biochemistry

Abstract

Abstract Mutation detection and mismatch repair investigations based on heteroduplex formation require a linear DNA structure. DNA branching, described previously under physiological conditions, has been analysed in the heteroduplex formation process. Symmetrical χ-structures were detected after heteroduplex formation by gel electrophoresis and electron microscopy. Buffer composition, DNA concentration and duplex end-sequences influence DNA branching. Duplexes with homologous central regions but non-complementary ends do not form hybrid heteroduplexes or hybrid Holliday junctions. Our results explain the requirements for efficient heteroduplex formation, which were previously determined empirically: special solution composition, optimal DNA concentration and GC clamps. This provides the theoretical background for further optimisation of the procedure.

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Lesovaya Ekaterina

73 publications, 685 citations

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Ryazan State Medical University named after Academician I.P. Pavlov

N.N. Blokhin National Medical Research Center of Oncology

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Neschastnova A. A. et al. Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation // Biological Chemistry. 2006. Vol. 387. No. 3. pp. 257-261.

GOST all authors (up to 50) Copy

Neschastnova A. A. Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation // Biological Chemistry. 2006. Vol. 387. No. 3. pp. 257-261.

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RIS Copy

TY - JOUR

DO - 10.1515/BC.2006.034

UR - https://doi.org/10.1515/BC.2006.034

TI - Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation

T2 - Biological Chemistry

AU - Neschastnova, Anna A

PY - 2006

DA - 2006/01/01

PB - Walter de Gruyter

SP - 257-261

IS - 3

VL - 387

PMID - 16542146

SN - 1431-6730

SN - 1437-4315

ER -

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@article{2006_Neschastnova,

author = {Anna A Neschastnova},

title = {Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation},

journal = {Biological Chemistry},

year = {2006},

volume = {387},

publisher = {Walter de Gruyter},

month = {jan},

url = {https://doi.org/10.1515/BC.2006.034},

number = {3},

pages = {257--261},

doi = {10.1515/BC.2006.034}

}

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Neschastnova, Anna A., et al. “Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation.” Biological Chemistry, vol. 387, no. 3, Jan. 2006, pp. 257-261. https://doi.org/10.1515/BC.2006.034.