EMBO Journal, volume 37, issue 7

Structure of a eukaryotic cytoplasmic pre‐40S ribosomal subunit

Scaiola Alain ¹

Peña Cohue ^{2, 3}

Weisser Melanie ¹

Böhringer Daniel ¹

Leibundgut Marc ¹

Klingauf Nerurkar Purnima ^{2, 3}

Gerhardy Stefan ³

Panse Vikram Govind ³

Ban Nenad ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Biology Institute of Molecular Biology and Biophysics ETH Zurich Zurich Switzerland |

Department of Biology Institute of Biochemistry ETH Zurich Zurich Switzerland |

Institute of Medical Microbiology University of Zurich Zurich Switzerland |

Publication type: Journal Article

Publication date: 2018-02-19

EMBO press

Journal: EMBO Journal

Quartile SCImago

Quartile WOS

Impact factor: 11.4

ISSN: 02614189, 14602075

DOI: 10.15252/embj.201798499

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PubMed ID: 29459436

General Biochemistry, Genetics and Molecular Biology

Molecular Biology

General Immunology and Microbiology

General Neuroscience

Abstract

Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre‐RNA. Using cryo‐electron microscopy (cryo‐EM), we have determined the structure of a yeast cytoplasmic pre‐40S particle in complex with Enp1, Ltv1, Rio2, Tsr1, and Pno1 assembly factors poised to initiate final maturation. The structure reveals that the pre‐rRNA adopts a highly distorted conformation of its 3′ major and 3′ minor domains stabilized by the binding of the assembly factors. This observation is consistent with a mechanism that involves concerted release of the assembly factors orchestrated by the folding of the rRNA in the head of the pre‐40S subunit during the final stages of maturation. Our results provide a structural framework for the coordination of the final maturation events that drive a pre‐40S particle toward the mature form capable of engaging in translation.

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Nature	Nature, 2, 2.56% Nature 2 publications, 2.56%
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Molecules	Molecules, 1, 1.28% Molecules 1 publication, 1.28%
Oncogenesis	Oncogenesis, 1, 1.28% Oncogenesis 1 publication, 1.28%
Nature Reviews Molecular Cell Biology	Nature Reviews Molecular Cell Biology, 1, 1.28% Nature Reviews Molecular Cell Biology 1 publication, 1.28%
Methods	Methods, 1, 1.28% Methods 1 publication, 1.28%
Structure	Structure, 1, 1.28% Structure 1 publication, 1.28%
Computational and Structural Biotechnology Journal	Computational and Structural Biotechnology Journal, 1, 1.28% Computational and Structural Biotechnology Journal 1 publication, 1.28%
PLoS Biology	PLoS Biology, 1, 1.28% PLoS Biology 1 publication, 1.28%
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Journal of Cellular and Molecular Medicine	Journal of Cellular and Molecular Medicine, 1, 1.28% Journal of Cellular and Molecular Medicine 1 publication, 1.28%
ACS Chemical Biology	ACS Chemical Biology, 1, 1.28% ACS Chemical Biology 1 publication, 1.28%
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Citations by publishers

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Springer Nature	Springer Nature, 10, 12.82% Springer Nature 10 publications, 12.82%
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American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 3, 3.85% American Association for the Advancement of Science (AAAS) 3 publications, 3.85%
eLife Sciences Publications	eLife Sciences Publications, 3, 3.85% eLife Sciences Publications 3 publications, 3.85%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 2, 2.56% Public Library of Science (PLoS) 2 publications, 2.56%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 2, 2.56% Proceedings of the National Academy of Sciences (PNAS) 2 publications, 2.56%
Acta Naturae Ltd	Acta Naturae Ltd, 1, 1.28% Acta Naturae Ltd 1 publication, 1.28%
Portland Press	Portland Press, 1, 1.28% Portland Press 1 publication, 1.28%
The Royal Society	The Royal Society, 1, 1.28% The Royal Society 1 publication, 1.28%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 1.28% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 1.28%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 1, 1.28% American Society for Biochemistry and Molecular Biology 1 publication, 1.28%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 1.28% American Chemical Society (ACS) 1 publication, 1.28%
EMBO press	EMBO press, 1, 1.28% EMBO press 1 publication, 1.28%
Annual Reviews	Annual Reviews, 1, 1.28% Annual Reviews 1 publication, 1.28%
Spandidos Publications	Spandidos Publications, 1, 1.28% Spandidos Publications 1 publication, 1.28%
	2 4 6 8 10

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Scaiola A. et al. Structure of a eukaryotic cytoplasmic pre‐40S ribosomal subunit // EMBO Journal. 2018. Vol. 37. No. 7.

GOST all authors (up to 50) Copy

Scaiola A., Peña C., Weisser M., Böhringer D., Leibundgut M., Klingauf Nerurkar P., Gerhardy S., Panse V. G., Ban N. Structure of a eukaryotic cytoplasmic pre‐40S ribosomal subunit // EMBO Journal. 2018. Vol. 37. No. 7.

RIS |

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RIS Copy

TY - JOUR

DO - 10.15252/embj.201798499

UR - https://doi.org/10.15252%2Fembj.201798499

TI - Structure of a eukaryotic cytoplasmic pre‐40S ribosomal subunit

T2 - EMBO Journal

AU - Scaiola, Alain

AU - Peña, Cohue

AU - Weisser, Melanie

AU - Böhringer, Daniel

AU - Leibundgut, Marc

AU - Klingauf Nerurkar, Purnima

AU - Gerhardy, Stefan

AU - Panse, Vikram Govind

AU - Ban, Nenad

PY - 2018

DA - 2018/02/19 00:00:00

PB - EMBO press

IS - 7

VL - 37

PMID - 29459436

SN - 0261-4189

SN - 1460-2075

ER -

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BibTex Copy

@article{2018_Scaiola,

author = {Alain Scaiola and Cohue Peña and Melanie Weisser and Daniel Böhringer and Marc Leibundgut and Purnima Klingauf Nerurkar and Stefan Gerhardy and Vikram Govind Panse and Nenad Ban},

title = {Structure of a eukaryotic cytoplasmic pre‐40S ribosomal subunit},

journal = {EMBO Journal},

year = {2018},

volume = {37},

publisher = {EMBO press},

month = {feb},

url = {https://doi.org/10.15252%2Fembj.201798499},

number = {7},

doi = {10.15252/embj.201798499}

}

Found error?

Publisher

EMBO press

Journal

EMBO Journal

Quartile SCImago

Quartile WOS

Impact factor

11.4

ISSN

02614189 (Print)
14602075 (Electronic)