Genetics, volume 213, issue 4, pages 1431-1446
The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop
Publication type: Journal Article
Publication date: 2019-12-01
PubMed ID:
31594794
Genetics
Abstract
orb encodes one of the two fly CPEB proteins. These widely conserved proteins bind to the 3′UTRs of target messenger RNAs (mRNAs) and activate or repress their translation. We show here that a positive autoregulatory loop driven by the orb gene propels the specification of oocyte identity in Drosophila egg chambers. Oocyte fate specification is mediated by a 3′UTR-dependent mechanism that concentrates orb mRNAs and proteins in one of the two pro-oocytes in the 16-cell germline cyst. When the orb 3′UTR is deleted, orb mRNA and protein fail to localize and all 16 cells become nurse cells. In wild type, the oocyte is specified when orb and other gene products concentrate in a single cell in region 2b of the germarium. A partially functional orb 3′UTR replacement delays oocyte specification until the egg chambers reach stage 2 of oogenesis. Before this point, orb mRNA and protein are unlocalized, as are other markers of oocyte identity, and the oocyte is not specified. After stage 2, ∼50% of the chambers successfully localize orb in a single cell, and this cell assumes oocyte identity. In the remaining chambers, the orb autoregulatory loop is not activated and no oocyte is formed. Finally, maintenance of oocyte identity requires continuous orb activity.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Barr J. et al. The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop // Genetics. 2019. Vol. 213. No. 4. pp. 1431-1446.
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Barr J., Gilmutdinov R., Wang L., Shidlovskii Y., Schedl P. The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop // Genetics. 2019. Vol. 213. No. 4. pp. 1431-1446.
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TY - JOUR
DO - 10.1534/genetics.119.302687
UR - https://doi.org/10.1534%2Fgenetics.119.302687
TI - The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop
T2 - Genetics
AU - Wang, Linus
AU - Shidlovskii, Yulii
AU - Barr, Justinn
AU - Gilmutdinov, Rudolf
AU - Schedl, Paul
PY - 2019
DA - 2019/12/01 00:00:00
PB - Genetics Society of America
SP - 1431-1446
IS - 4
VL - 213
PMID - 31594794
SN - 0016-6731
SN - 1943-2631
ER -
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@article{2019_Barr,
author = {Linus Wang and Yulii Shidlovskii and Justinn Barr and Rudolf Gilmutdinov and Paul Schedl},
title = {The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop},
journal = {Genetics},
year = {2019},
volume = {213},
publisher = {Genetics Society of America},
month = {dec},
url = {https://doi.org/10.1534%2Fgenetics.119.302687},
number = {4},
pages = {1431--1446},
doi = {10.1534/genetics.119.302687}
}
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MLA
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Barr, Justinn, et al. “The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3′UTR-Dependent Autoregulatory Loop.” Genetics, vol. 213, no. 4, Dec. 2019, pp. 1431-1446. https://doi.org/10.1534%2Fgenetics.119.302687.
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