Open Access
Brazilian Journal of Medical and Biological Research, volume 48, issue 5, pages 382-391
Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
1
Universidade de São Paulo, Brasil
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Publication type: Journal Article
Publication date: 2015-05-01
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Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 2.3
ISSN: 0100879X, 1414431X, 16784510
PubMed ID:
25760027
Biochemistry
General Medicine
Cell Biology
Biophysics
General Pharmacology, Toxicology and Pharmaceutics
General Neuroscience
Physiology
Immunology
Abstract
Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both in mitosis as well as in interphase. The MNs were able to replicate their DNA and this process seemed to be non-synchronous with the main cell nuclei. The presence of ErbB1 gene in the MNs was evaluated by fluorescent in situ hybridization (FISH). ErbB1 sequences were detected in the MNs, but a relation between the MNs formation and extrusion of amplified ErbB1could not be established. The present study sought to elucidate the meaning of MNs formation and its association with the elimination of oncogenes or other amplified sequences from the tumor cells.
Citations by journals
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Russian Chemical Bulletin
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Russian Chemical Bulletin
1 publication, 25%
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Oncotarget
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1 publication, 25%
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Oncogene
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Oncogene
1 publication, 25%
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Biochemistry (Moscow)
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Biochemistry (Moscow)
1 publication, 25%
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1
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Citations by publishers
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1
2
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Springer Nature
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Springer Nature
2 publications, 50%
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Impact Journals
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Impact Journals
1 publication, 25%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 25%
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1
2
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Lauand C. et al. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells // Brazilian Journal of Medical and Biological Research. 2015. Vol. 48. No. 5. pp. 382-391.
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Lauand C., Niero E., Dias V. M., Machado Santelli G. M. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells // Brazilian Journal of Medical and Biological Research. 2015. Vol. 48. No. 5. pp. 382-391.
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TY - JOUR
DO - 10.1590/1414-431x20144262
UR - https://doi.org/10.1590%2F1414-431x20144262
TI - Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
T2 - Brazilian Journal of Medical and Biological Research
AU - Lauand, C.
AU - Niero, E.L.
AU - Dias, V M
AU - Machado Santelli, G M
PY - 2015
DA - 2015/05/01 00:00:00
SP - 382-391
IS - 5
VL - 48
PMID - 25760027
SN - 0100-879X
SN - 1414-431X
SN - 1678-4510
ER -
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@article{2015_Lauand,
author = {C. Lauand and E.L. Niero and V M Dias and G M Machado Santelli},
title = {Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells},
journal = {Brazilian Journal of Medical and Biological Research},
year = {2015},
volume = {48},
month = {may},
url = {https://doi.org/10.1590%2F1414-431x20144262},
number = {5},
pages = {382--391},
doi = {10.1590/1414-431x20144262}
}
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MLA
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Lauand, C., et al. “Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells.” Brazilian Journal of Medical and Biological Research, vol. 48, no. 5, May. 2015, pp. 382-391. https://doi.org/10.1590%2F1414-431x20144262.