Open Access
Oncotarget, volume 7, issue 23, pages 34930-34941
Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer
Gorges Tobias M.
1
,
Riethdorf Sabine
1
,
von Ahsen Oliver
2
,
Nastały Paulina
1
,
Röck Katharina
1
,
Boede Marcel
3
,
Peine Sven
4
,
Kuske Andra
1
,
Schmid Elke
2
,
Kneip Christoph
2
,
KöNIG FRANK
3
,
Rudolph Marion
2
,
1
Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
|
2
BPH-DD-TRG-CIPL-Biomarker Research, Bayer Pharma AG, Berlin, Germany
|
3
ATURO, Urology Practice, Berlin, Germany
|
4
Department of Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
|
Publication type: Journal Article
Publication date: 2016-04-26
PubMed ID:
27145459
Oncology
Abstract
The prostate specific membrane antigen (PSMA) is the only clinically validated marker for therapeutic decisions in prostate cancer (PC). Characterization of circulating tumor cells (CTCs) obtained from the peripheral blood of PC patients might provide an alternative to tissue biopsies called “liquid biopsy”. The aim of this study was to develop a reliable assay for the determination of PSMA on CTCs. PSMA expression was analyzed on tissue samples (cohort one, n = 75) and CTCs from metastatic PC patients (cohort two, n = 29). Specific signals for the expression of PSMA could be seen for different prostate cancer cell line cells (PC3, LaPC4, 22Rv1, and LNCaP) by Western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and FACS. PSMA expression was found to be significantly increased in patients with higher Gleason grade (p = 0.0011) and metastases in lymph nodes (p = 0.0000085) or bone (p = 0.0020) (cohort one). In cohort two, CTCs were detectable in 20 out of 29 samples (69 %, range from 1 - 1000 cells). Twelve out of 20 CTC-positive patients showed PSMA-positive CTCs (67 %, score 1+ to 3+). We found intra-patient heterogeneity regarding the PSMA status between CTCs and the corresponding primary tumors. The results of our study could help to address the question whether treatment decisions based on CTC PSMA profiling will lead to a measurable benefit in clinical outcome for prostate cancer patients in the near future.
Citations by journals
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2 publications, 2.86%
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2 publications, 2.86%
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1 publication, 1.43%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Gorges T. M. et al. Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer // Oncotarget. 2016. Vol. 7. No. 23. pp. 34930-34941.
GOST all authors (up to 50)
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Gorges T. M., Riethdorf S., von Ahsen O., Nastały P., Röck K., Boede M., Peine S., Kuske A., Schmid E., Kneip C., KöNIG F., Rudolph M., Pantel K. Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer // Oncotarget. 2016. Vol. 7. No. 23. pp. 34930-34941.
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TY - JOUR
DO - 10.18632/oncotarget.9004
UR - https://doi.org/10.18632%2Foncotarget.9004
TI - Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer
T2 - Oncotarget
AU - Gorges, Tobias M.
AU - Riethdorf, Sabine
AU - von Ahsen, Oliver
AU - Nastały, Paulina
AU - Röck, Katharina
AU - Boede, Marcel
AU - Peine, Sven
AU - Kuske, Andra
AU - Schmid, Elke
AU - Kneip, Christoph
AU - KöNIG, FRANK
AU - Rudolph, Marion
AU - Pantel, Klaus
PY - 2016
DA - 2016/04/26 00:00:00
PB - Impact Journals
SP - 34930-34941
IS - 23
VL - 7
PMID - 27145459
SN - 1949-2553
ER -
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@article{2016_Gorges,
author = {Tobias M. Gorges and Sabine Riethdorf and Oliver von Ahsen and Paulina Nastały and Katharina Röck and Marcel Boede and Sven Peine and Andra Kuske and Elke Schmid and Christoph Kneip and FRANK KöNIG and Marion Rudolph and Klaus Pantel},
title = {Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer},
journal = {Oncotarget},
year = {2016},
volume = {7},
publisher = {Impact Journals},
month = {apr},
url = {https://doi.org/10.18632%2Foncotarget.9004},
number = {23},
pages = {34930--34941},
doi = {10.18632/oncotarget.9004}
}
Cite this
MLA
Copy
Gorges, Tobias M., et al. “Heterogeneous PSMA expression on circulating tumor cells - a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer.” Oncotarget, vol. 7, no. 23, Apr. 2016, pp. 34930-34941. https://doi.org/10.18632%2Foncotarget.9004.
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