Open Access
Infection and Drug Resistance, volume Volume 12, pages 261-272
Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa
Feng Xinyuan
1
,
Zhang Zhiqi
2
,
Li Xiaoxia
3
,
Song Yan
3
,
Kang Jianbang
3
,
Yin Donghong
3
,
Gao Yating
,
Gao Yating
3
,
Shi Nan
1
,
Duan Jinju
,
Song Junli
3
Publication type: Journal Article
Publication date: 2019-02-07
Journal:
Infection and Drug Resistance
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.9
ISSN: 11786973
PubMed ID:
30804676
Pharmacology
Infectious Diseases
Pharmacology (medical)
Abstract
Purpose To investigate the main molecular resistance mechanisms to fluoroquinolones (FQs) in Pseudomonas aeruginosa and also to investigate the effect of time and concentration on mutations in resistance genes. Materials and methods The clinical isolates of P. aeruginosa which are sensitive to ciprofloxacin (CIP) or levofloxacin (LEV) were collected. The isolates were incubated with different concentrations of CIP or LEV for 5 days and the minimal inhibitory concentrations (MICs) of CIP, LEV and ofloxacin (OFX) were measured. The MIC of FQs to P. aeruginosa was measured by the agar dilution method. FQ resistance determining regions of gyrA, gyrB, parC and parE were amplified by PCR, and mutations in four genes were explored using sequence analysis with the Snapgene software. The relative expression levels of two efflux pumps genes (mexA and mexE) were measured by quantitative reverse transcription PCR. Results A total of eleven isolates were collected from the Second Hospital of Shanxi Medical University. Amino acid alterations in gyrA and gyrB were mainly detected in resistant mutants, and the percentage of strains with amino acid alterations in gyrB was significantly higher than that in gyrA (P<0.001). MICs of strains with mutations both in gyrA and gyrB were not significantly higher than those of strains with mutations only in gyrB (P>0.05). No amino acid alterations were detected in genes of parC and parE. In both gyrA and gyrB, the number of amino acid alterations increased with incubation time prolonged and increased with increasing incubation concentration. Conclusion CIP was more competent than LEV in making P. aeruginosa resistant to in vitro selection. Mutations occurring in gyrB played an important role in FQ resistance of P. aeruginosa in vitro selection.
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- Statistics recalculated weekly.
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Feng X. et al. Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa // Infection and Drug Resistance. 2019. Vol. Volume 12. pp. 261-272.
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Feng X., Zhang Z., Li X., Song Y., Kang J., Yin D., Gao Y., Gao Y., Shi N., Duan J., Song J. Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa // Infection and Drug Resistance. 2019. Vol. Volume 12. pp. 261-272.
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TY - JOUR
DO - 10.2147/IDR.S182272
UR - https://doi.org/10.2147%2FIDR.S182272
TI - Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa
T2 - Infection and Drug Resistance
AU - Feng, Xinyuan
AU - Zhang, Zhiqi
AU - Li, Xiaoxia
AU - Song, Yan
AU - Kang, Jianbang
AU - Yin, Donghong
AU - Gao, Yating
AU - Shi, Nan
AU - Duan, Jinju
AU - Gao, Yating
AU - Song, Junli
PY - 2019
DA - 2019/02/07 00:00:00
PB - Dove Medical Press
SP - 261-272
VL - Volume 12
PMID - 30804676
SN - 1178-6973
ER -
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@article{2019_Feng,
author = {Xinyuan Feng and Zhiqi Zhang and Xiaoxia Li and Yan Song and Jianbang Kang and Donghong Yin and Yating Gao and Nan Shi and Jinju Duan and Yating Gao and Junli Song},
title = {Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa},
journal = {Infection and Drug Resistance},
year = {2019},
volume = {Volume 12},
publisher = {Dove Medical Press},
month = {feb},
url = {https://doi.org/10.2147%2FIDR.S182272},
pages = {261--272},
doi = {10.2147/IDR.S182272}
}