Open Access
OncoTargets and Therapy, volume Volume 13, pages 12083-12096
Identification and Development of Long Non‐coding RNA Associated Regulatory Network in Pancreatic Adenocarcinoma
Publication type: Journal Article
Publication date: 2020-11-22
Journal:
OncoTargets and Therapy
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 4
ISSN: 11786930
PubMed ID:
33262608
Oncology
Pharmacology (medical)
Abstract
Background and Aims Pancreatic adenocarcinoma (PAAD) is the most lethal cancer type around the world. With the in-depth exploration of the function of long non‐coding RNAs (lncRNAs), the competing endogenous RNA (ceRNA) mechanism has shown its potential to partially reveal the pathogenesis of PAAD. This study aimed to construct a lncRNA‐associated ceRNA network and explore ceRNA regulatory axes with experimental and prognostic value in PAAD. Methods First, we applied differential expression analysis in the TCGA_PAAD dataset. Then, interaction analysis and survival analysis in multiple RNA interaction databases were conducted to construct a ceRNA network. Finally, a potential regulatory axis was validated using clinical samples and cell lines by quantitative realtime PCR (qRT‐PCR). Results A ceRNA network comprising 13 lncRNAs, 96 miRNAs, and 30 mRNAs was successfully constructed. Survival analysis further narrowed this network to five lncRNAs, three miRNAs, and seven mRNAs, which were significantly associated with patients’ overall survival. A potential regulatory axis CASC8-miR-129-5p-TOB1 was further experimentally validated. The expression of these genes was associated with clinicopathological factors and their expression trend was consistent with ceRNA mechanism. Specifically, knockdown of lncRNA-CASC8 led to the overexpression of miR-129-5p and down-regulation of TOB1, while overexpression of CASC8 showed opposite effects. Conclusion This novel ceRNA regulatory network could provide new insight into the pathogenesis of PAAD. The new regulatory axis CASC8-miR-129-5p-TOB1 might serve as a potential therapeutic target for patients.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zhu W. et al. Identification and Development of Long Non‐coding RNA Associated Regulatory Network in Pancreatic Adenocarcinoma // OncoTargets and Therapy. 2020. Vol. Volume 13. pp. 12083-12096.
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Zhu W., Gao W., Deng Y., Xiao Yu., Zhu H. Identification and Development of Long Non‐coding RNA Associated Regulatory Network in Pancreatic Adenocarcinoma // OncoTargets and Therapy. 2020. Vol. Volume 13. pp. 12083-12096.
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TY - JOUR
DO - 10.2147/OTT.S265036
UR - https://doi.org/10.2147%2FOTT.S265036
TI - Identification and Development of Long Non‐coding RNA Associated Regulatory Network in Pancreatic Adenocarcinoma
T2 - OncoTargets and Therapy
AU - Zhu, Wenjuan
AU - Gao, Wenzhe
AU - Deng, Yanyao
AU - Zhu, Hongwei
AU - Xiao, Yu
PY - 2020
DA - 2020/11/22 00:00:00
PB - Dove Medical Press
SP - 12083-12096
VL - Volume 13
PMID - 33262608
SN - 1178-6930
ER -
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@article{2020_Zhu,
author = {Wenjuan Zhu and Wenzhe Gao and Yanyao Deng and Hongwei Zhu and Yu Xiao},
title = {Identification and Development of Long Non‐coding RNA Associated Regulatory Network in Pancreatic Adenocarcinoma},
journal = {OncoTargets and Therapy},
year = {2020},
volume = {Volume 13},
publisher = {Dove Medical Press},
month = {nov},
url = {https://doi.org/10.2147%2FOTT.S265036},
pages = {12083--12096},
doi = {10.2147/OTT.S265036}
}