Open Access
Acta Naturae, volume 4, issue 1, pages 70-73
Hansenula Polymorpha TERT: A Telomerase Catalytic Subunit Isolated in Recombinant Form with Limited Reverse Transcriptase Activity
Publication type: Journal Article
Publication date: 2012-03-15
Abstract
Telomerase is a ribonucleoprotein, the main function of which is to synthesize telomeres, i.e. repetitive sequences which are localized at the ends of eukaryotic chromosomes. Telomerase maintains the stability of the genome in eukaryotic cells by replicating chromosomal ends. The structural and functional investigation of the telomerase complex is significantly restricted due to difficulties connected with the isolation of its main catalytic subunit in recombinant form. Herein, we describe a method developed for the isolation of the recombinant telomerase reverse transcriptase from thermotolerant yeastHansenula polymorpha. A functional test performed for the isolated protein and the RNA/DNA duplex, simulating the interaction of telomerase RNA and telomere, reveals that the isolated catalytic subunit of telomerase possesses limited reverse transcriptase activity.
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Citations by publishers
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Smekalova E.M. et al. Hansenula Polymorpha TERT: A Telomerase Catalytic Subunit Isolated in Recombinant Form with Limited Reverse Transcriptase Activity // Acta Naturae. 2012. Vol. 4. No. 1. pp. 70-73.
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Smekalova E.M., Petrova O.A., Zvereva M.I., Dontsova O.A. Hansenula Polymorpha TERT: A Telomerase Catalytic Subunit Isolated in Recombinant Form with Limited Reverse Transcriptase Activity // Acta Naturae. 2012. Vol. 4. No. 1. pp. 70-73.
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TY - JOUR
DO - 10.32607/20758251-2012-4-1-70-73
UR - https://doi.org/10.32607%2F20758251-2012-4-1-70-73
TI - Hansenula Polymorpha TERT: A Telomerase Catalytic Subunit Isolated in Recombinant Form with Limited Reverse Transcriptase Activity
T2 - Acta Naturae
AU - Smekalova, E M
AU - Petrova, O A
AU - Zvereva, M I
AU - Dontsova, O A
PY - 2012
DA - 2012/03/15 00:00:00
PB - Acta Naturae Ltd
SP - 70-73
IS - 1
VL - 4
SN - 2075-8251
SN - 2075-8243
ER -
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@article{2012
author = {E M Smekalova and O A Petrova and M I Zvereva and O A Dontsova},
title = {Hansenula Polymorpha TERT: A Telomerase Catalytic Subunit Isolated in Recombinant Form with Limited Reverse Transcriptase Activity},
journal = {Acta Naturae},
year = {2012},
volume = {4},
publisher = {Acta Naturae Ltd},
month = {mar},
url = {https://doi.org/10.32607%2F20758251-2012-4-1-70-73},
number = {1},
pages = {70--73},
doi = {10.32607/20758251-2012-4-1-70-73}
}
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