Open Access
Acta Naturae, volume 12, issue 1, pages 42-50
Simple recommendations for improving efficiency in generating genome-edited mice
Publication type: Journal Article
Publication date: 2020-04-16
PubMed ID:
32477597
Biochemistry
Molecular Biology
Molecular Medicine
Biotechnology
Abstract
The generation of transgenic model organisms (primarily mice) is an integral part of modern fundamental and applied research. Simple techniques based on the biology of these laboratory rodents can often increase efficiency when generating genome-edited mouse strains. In this study, we share our three years of experience in the optimization of mouse genome editing based on microinjection of CRISPR/Cas9 components into ca. 10,000 zygotes. We tested a number of techniques meant to improve efficiency in generating knockout mice, such as optimization of the superovulation method and choosing the optimal mouse strains to be used as zygote donors and foster mothers. The presented results might be useful to laboratories aiming to quickly and efficiently create new mouse strains with tailored genome editing.
Citations by journals
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
3 publications, 42.86%
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Molecular Biology Reports
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Molecular Biology Reports
2 publications, 28.57%
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Biochimie
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Biochimie
1 publication, 14.29%
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Biochemistry (Moscow)
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Biochemistry (Moscow)
1 publication, 14.29%
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 42.86%
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Springer Nature
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Springer Nature
2 publications, 28.57%
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Elsevier
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Elsevier
1 publication, 14.29%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 14.29%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Averina O. A. et al. Simple recommendations for improving efficiency in generating genome-edited mice // Acta Naturae. 2020. Vol. 12. No. 1. pp. 42-50.
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Averina O. A., Vysokikh M. Y., Permyakov O. A., Sergiev P. V. Simple recommendations for improving efficiency in generating genome-edited mice // Acta Naturae. 2020. Vol. 12. No. 1. pp. 42-50.
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TY - JOUR
DO - 10.32607/actanaturae.10937
UR - https://doi.org/10.32607%2Factanaturae.10937
TI - Simple recommendations for improving efficiency in generating genome-edited mice
T2 - Acta Naturae
AU - Averina, Olga A
AU - Vysokikh, M Y
AU - Permyakov, O A
AU - Sergiev, P V
PY - 2020
DA - 2020/04/16 00:00:00
PB - Acta Naturae Ltd
SP - 42-50
IS - 1
VL - 12
PMID - 32477597
SN - 2075-8251
SN - 2075-8243
ER -
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@article{2020_Averina,
author = {Olga A Averina and M Y Vysokikh and O A Permyakov and P V Sergiev},
title = {Simple recommendations for improving efficiency in generating genome-edited mice},
journal = {Acta Naturae},
year = {2020},
volume = {12},
publisher = {Acta Naturae Ltd},
month = {apr},
url = {https://doi.org/10.32607%2Factanaturae.10937},
number = {1},
pages = {42--50},
doi = {10.32607/actanaturae.10937}
}
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MLA
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Averina, Olga A., et al. “Simple recommendations for improving efficiency in generating genome-edited mice.” Acta Naturae, vol. 12, no. 1, Apr. 2020, pp. 42-50. https://doi.org/10.32607%2Factanaturae.10937.
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