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Frontiers in Bioengineering and Biotechnology, volume 9

Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

Savelyev Alexander G. ^{1, 2}

Sochilina Anastasia V ^{2, 3}

Akasov Roman A ^{1, 2, 3}

Mironov Anton V ²

Kapitannikova Alina Yu ¹

Borodina Tatiana N ²

Sholina Natalya V ^{1, 2}

Khaydukov Kirill V. ²

Zvyagin Andrei V ^{1, 3, 4}

Generalova Alla N ^{2, 3}

Khaydukov Evgeny V ^{1, 2, 3}

Hide authors affiliations Show authors affiliations: 4 affiliations

Center of Biomedical Engineering, Institute of Molecular Medicine, Sechenov University, Russia |

Federal Scientific Research Centre ?Crystallography and Photonics? Russian Academy of Sciences, Russia |

Shemyakin-Ovchinnikov Institute of Bioorganic ?hemistry RAS, Russia |

⁴

MQ Photonics Centre, Faculty of Science and Engineering, Macquarie University, Australia |

Publication type: Journal Article

Publication date: 2021-12-03

Frontiers Media S.A.

Journal: Frontiers in Bioengineering and Biotechnology

Quartile SCImago

Quartile WOS

Impact factor: 5.7

ISSN: 22964185

DOI: 10.3389/fbioe.2021.783834

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PubMed ID: 34926429

Biotechnology

Bioengineering

Histology

Biomedical Engineering

Abstract

Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry.

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	1
Frontiers in Bioengineering and Biotechnology	Frontiers in Bioengineering and Biotechnology, 1, 20% Frontiers in Bioengineering and Biotechnology 1 publication, 20%
Life	Life, 1, 20% Life 1 publication, 20%
ACS applied materials & interfaces	ACS applied materials & interfaces, 1, 20% ACS applied materials & interfaces 1 publication, 20%
Russian Chemical Reviews	Russian Chemical Reviews, 1, 20% Russian Chemical Reviews 1 publication, 20%
Advanced Science	Advanced Science, 1, 20% Advanced Science 1 publication, 20%
	1

Citations by publishers

	1
Frontiers Media S.A.	Frontiers Media S.A., 1, 20% Frontiers Media S.A. 1 publication, 20%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 20% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 20%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 20% American Chemical Society (ACS) 1 publication, 20%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 1, 20% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 1 publication, 20%
Wiley	Wiley, 1, 20% Wiley 1 publication, 20%
	1

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Savelyev A. G. et al. Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication // Frontiers in Bioengineering and Biotechnology. 2021. Vol. 9.

GOST all authors (up to 50) Copy

Savelyev A. G., Sochilina A. V., Akasov R. A., Mironov A. V., Kapitannikova A. Yu., Borodina T. N., Sholina N. V., Khaydukov K. V., Zvyagin A. V., Generalova A. N., Khaydukov E. V. Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication // Frontiers in Bioengineering and Biotechnology. 2021. Vol. 9.

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RIS Copy

TY - JOUR

DO - 10.3389/fbioe.2021.783834

UR - https://doi.org/10.3389%2Ffbioe.2021.783834

TI - Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

T2 - Frontiers in Bioengineering and Biotechnology

AU - Savelyev, Alexander G.

AU - Sochilina, Anastasia V

AU - Akasov, Roman A

AU - Mironov, Anton V

AU - Kapitannikova, Alina Yu

AU - Borodina, Tatiana N

AU - Sholina, Natalya V

AU - Khaydukov, Kirill V.

AU - Zvyagin, Andrei V

AU - Generalova, Alla N

AU - Khaydukov, Evgeny V

PY - 2021

DA - 2021/12/03 00:00:00

PB - Frontiers Media S.A.

VL - 9

PMID - 34926429

SN - 2296-4185

ER -

BibTex

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BibTex Copy

@article{2021_Savelyev,

author = {Alexander G. Savelyev and Anastasia V Sochilina and Roman A Akasov and Anton V Mironov and Alina Yu Kapitannikova and Tatiana N Borodina and Natalya V Sholina and Kirill V. Khaydukov and Andrei V Zvyagin and Alla N Generalova and Evgeny V Khaydukov},

title = {Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication},

journal = {Frontiers in Bioengineering and Biotechnology},

year = {2021},

volume = {9},

publisher = {Frontiers Media S.A.},

month = {dec},

url = {https://doi.org/10.3389%2Ffbioe.2021.783834},

doi = {10.3389/fbioe.2021.783834}

}

Found error?

Publisher

Frontiers Media S.A.

Journal

Frontiers in Bioengineering and Biotechnology

Quartile SCImago

Quartile WOS

Impact factor

5.7

ISSN

22964185 (Electronic)

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