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Frontiers in Cell and Developmental Biology, volume 9

Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure

Zakirov Amir N ^{1, 2}

Sosnovskaya Sophie ^{1, 2}

Ryumina Ekaterina D ^{1, 3}

Kharybina Ekaterina ^{1, 2}

Strelkova Olga S ¹

Zhironkina Oxana A ¹

Golyshev Sergei A ¹

Moiseenko Andrey ⁴

Kireev Igor I ^{1, 2}

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Department of Electron Microscopy, Russia |

Chair of Cell Biology and Histology, Russia |

Faculty of Bioengineering and Bioinformatics, Russia |

⁴

Laboratory of Electron Microscopy, Russia |

Publication type: Journal Article

Publication date: 2022-01-31

Frontiers Media S.A.

Journal: Frontiers in Cell and Developmental Biology

Quartile SCImago

Quartile WOS

Impact factor: 5.5

ISSN: 2296634X

DOI: 10.3389/fcell.2021.784440

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PubMed ID: 35174159

Cell Biology

Developmental Biology

Abstract

A detailed understanding of the principles of the structural organization of genetic material is of great importance for elucidating the mechanisms of differential regulation of genes in development. Modern ideas about the spatial organization of the genome are based on a microscopic analysis of chromatin structure and molecular data on DNA–DNA contact analysis using Chromatin conformation capture (3C) technology, ranging from the “polymer melt” model to a hierarchical folding concept. Heterogeneity of chromatin structure depending on its functional state and cell cycle progression brings another layer of complexity to the interpretation of structural data and requires selective labeling of various transcriptional states under nondestructive conditions. Here, we use a modified approach for replication timing-based metabolic labeling of transcriptionally active chromatin for ultrastructural analysis. The method allows pre-embedding labeling of optimally structurally preserved chromatin, thus making it compatible with various 3D-TEM techniques including electron tomography. By using variable pulse duration, we demonstrate that euchromatic genomic regions adopt a fiber-like higher-order structure of about 200 nm in diameter (chromonema), thus providing support for a hierarchical folding model of chromatin organization as well as the idea of transcription and replication occurring on a highly structured chromatin template.

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Cell Reports	Cell Reports, 1, 20% Cell Reports 1 publication, 20%
Frontiers in Neuroscience	Frontiers in Neuroscience, 1, 20% Frontiers in Neuroscience 1 publication, 20%
Chromosoma	Chromosoma, 1, 20% Chromosoma 1 publication, 20%
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Elsevier	Elsevier, 1, 20% Elsevier 1 publication, 20%
Frontiers Media S.A.	Frontiers Media S.A., 1, 20% Frontiers Media S.A. 1 publication, 20%
Springer Nature	Springer Nature, 1, 20% Springer Nature 1 publication, 20%
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Zakirov A. N. et al. Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure // Frontiers in Cell and Developmental Biology. 2022. Vol. 9.

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Zakirov A. N., Sosnovskaya S., Ryumina E. D., Kharybina E., Strelkova O. S., Zhironkina O. A., Golyshev S. A., Moiseenko A., Kireev I. I. Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure // Frontiers in Cell and Developmental Biology. 2022. Vol. 9.

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RIS Copy

TY - JOUR

DO - 10.3389/fcell.2021.784440

UR - https://doi.org/10.3389%2Ffcell.2021.784440

TI - Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure

T2 - Frontiers in Cell and Developmental Biology

AU - Zakirov, Amir N

AU - Sosnovskaya, Sophie

AU - Ryumina, Ekaterina D

AU - Kharybina, Ekaterina

AU - Strelkova, Olga S

AU - Zhironkina, Oxana A

AU - Golyshev, Sergei A

AU - Moiseenko, Andrey

AU - Kireev, Igor I

PY - 2022

DA - 2022/01/31 00:00:00

PB - Frontiers Media S.A.

VL - 9

PMID - 35174159

SN - 2296-634X

ER -

BibTex

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@article{2022_Zakirov,

author = {Amir N Zakirov and Sophie Sosnovskaya and Ekaterina D Ryumina and Ekaterina Kharybina and Olga S Strelkova and Oxana A Zhironkina and Sergei A Golyshev and Andrey Moiseenko and Igor I Kireev},

title = {Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure},

journal = {Frontiers in Cell and Developmental Biology},

year = {2022},

volume = {9},

publisher = {Frontiers Media S.A.},

month = {jan},

url = {https://doi.org/10.3389%2Ffcell.2021.784440},

doi = {10.3389/fcell.2021.784440}

}

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Publisher

Frontiers Media S.A.

Journal

Frontiers in Cell and Developmental Biology

Quartile SCImago

Quartile WOS

Impact factor

5.5

ISSN

2296634X (Electronic)

Labs

Department of Electron Microscopy

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