Open Access
Frontiers in Cellular and Infection Microbiology, volume 7
Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni
Sałamaszyńska Guz Agnieszka
1
,
Rose Simon
2
,
Lykkebo Claus A
2
,
Taciak Bartłomiej
3
,
Bącal Paweł
4
,
Uśpieński Tomasz
1
,
Douthwaite Stephen
2
1
Division of Microbiology, Department of Pre-Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
|
3
Division of Physiology, Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
|
4
Laboratory of Theory and Applications of Electrodes, Faculty of Chemistry, University of Warsaw, Poland
|
Publication type: Journal Article
Publication date: 2018-01-18
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.7
ISSN: 22352988
PubMed ID:
29404277
Microbiology (medical)
Microbiology
Infectious Diseases
Immunology
Abstract
Numerous bacterial pathogens express an ortholog of the enzyme TlyA, which is an rRNA 2´-O-methyltransferase associated with resistance to cyclic peptide antibiotics such as capreomycin. Several other virulence traits have also been attributed to TlyA, and these appear to be unrelated to its methyltransferase activity. The bacterial pathogen Campylobacter jejuni possesses the TlyA homolog Cj0588, which has been shown to contribute to virulence. Here, we investigate the mechanism of Cj0588 action and demonstrate that it is a type I homolog of TlyA that 2´-O-methylates 23S rRNA nucleotide C1920. This same specific function is retained by Cj0588 both in vitro and also when expressed in Escherichia coli. Deletion of the cj0588 gene in C. jejuni or substitution with alanine of K80, D162 or K188 in the catalytic center of the enzyme cause complete loss of 2´-O-methylation activity. Cofactor interactions remain unchanged and binding affinity to the ribosomal substrate is only slightly reduced, indicating that the inactivated proteins are folded correctly. The substitution mutations thus dissociate the 2´-O-methylation function of Cj0588/TlyA from any other putative roles that the protein might play. C. jejuni strains expressing catalytically inactive versions of Cj0588 have the same phenotype as cj0588-null mutants, and show altered tolerance to capreomycin due to perturbed ribosomal subunit association, reduced motility and impaired ability to form biofilms. These functions are reestablished when methyltransferase activity is restored and we conclude that the contribution of Cj0588 to virulence in C. jejuni is a consequence of the enzyme’s ability to methylate its rRNA.
Citations by journals
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2
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Frontiers in Cellular and Infection Microbiology
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Frontiers in Cellular and Infection Microbiology
2 publications, 16.67%
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1 publication, 8.33%
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1 publication, 8.33%
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Animals
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1 publication, 8.33%
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1 publication, 8.33%
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Biomolecules
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1 publication, 8.33%
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Biochimica et Biophysica Acta - General Subjects
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Biochimica et Biophysica Acta - General Subjects
1 publication, 8.33%
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South African Journal of Botany
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South African Journal of Botany
1 publication, 8.33%
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Cellular Microbiology
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1 publication, 8.33%
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Veterinary Microbiology
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Veterinary Microbiology
1 publication, 8.33%
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RNA
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1 publication, 8.33%
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1
2
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Citations by publishers
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1
2
3
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 25%
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Frontiers Media S.A.
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Frontiers Media S.A.
3 publications, 25%
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Elsevier
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Elsevier
3 publications, 25%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 8.33%
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Wiley
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Wiley
1 publication, 8.33%
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Cold Spring Harbor Laboratory
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Cold Spring Harbor Laboratory
1 publication, 8.33%
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1
2
3
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Sałamaszyńska Guz A. et al. Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni // Frontiers in Cellular and Infection Microbiology. 2018. Vol. 7.
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Sałamaszyńska Guz A., Rose S., Lykkebo C. A., Taciak B., Bącal P., Uśpieński T., Douthwaite S. Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni // Frontiers in Cellular and Infection Microbiology. 2018. Vol. 7.
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TY - JOUR
DO - 10.3389/fcimb.2017.00533
UR - https://doi.org/10.3389%2Ffcimb.2017.00533
TI - Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni
T2 - Frontiers in Cellular and Infection Microbiology
AU - Sałamaszyńska Guz, Agnieszka
AU - Rose, Simon
AU - Lykkebo, Claus A
AU - Taciak, Bartłomiej
AU - Bącal, Paweł
AU - Uśpieński, Tomasz
AU - Douthwaite, Stephen
PY - 2018
DA - 2018/01/18 00:00:00
PB - Frontiers Media S.A.
VL - 7
PMID - 29404277
SN - 2235-2988
ER -
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@article{2018_Sałamaszyńska Guz,
author = {Agnieszka Sałamaszyńska Guz and Simon Rose and Claus A Lykkebo and Bartłomiej Taciak and Paweł Bącal and Tomasz Uśpieński and Stephen Douthwaite},
title = {Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni},
journal = {Frontiers in Cellular and Infection Microbiology},
year = {2018},
volume = {7},
publisher = {Frontiers Media S.A.},
month = {jan},
url = {https://doi.org/10.3389%2Ffcimb.2017.00533},
doi = {10.3389/fcimb.2017.00533}
}