Open Access

Frontiers in Molecular Neuroscience, volume 15

Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d

Rysenkova Karina D ^{1, 2}

Troyanovskiy Konstantin E ¹

Klimovich Polina S ^{1, 2}

Bulyakova Taisiya R ¹

Shelomentseva Ekaterina M ¹

Shmakova Anna A. ^{1, 2}

Tanygina Daria Yu ¹

Ivashkina Olga I ^{3, 4, 5}

Anokhin Konstantin V ^{4, 5}

Karagyaur Maxim N ¹

Zvereva Maria I. ⁶

Rubina Kseniya A ¹

Tkachuk Vsevolod A. ^{1, 2}

Semina Ekaterina V ^{1, 2}

Hide authors affiliations Show authors affiliations: 6 affiliations

Faculty of Medicine, Lomonosov Moscow State University, Russia |

Institute of Experimental Cardiology, National Medical Research Centre of Cardiology named after academician E.I. Chazov, Russia |

Laboratory of Neuroscience, National Research Center “Kurchatov Institute”, Russia |

⁴

Laboratory of Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, Russia |

⁵

Institute for Advanced Brain Studies, Lomonosov Moscow State University, Russia |

⁶

Department of Chemistry, Lomonosov Moscow State University, Russia |

Publication type: Journal Article

Publication date: 2022-07-06

Frontiers Media S.A.

Journal: Frontiers in Molecular Neuroscience

Quartile SCImago

Quartile WOS

Impact factor: 4.8

ISSN: 16625099

DOI: 10.3389/fnmol.2022.865858

Copy DOI

PubMed ID: 35875662

Molecular Biology

Cellular and Molecular Neuroscience

Abstract

Urokinase receptor (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored receptor of urokinase (uPA), which is involved in brain development, nerve regeneration, wound healing and tissue remodeling. We have recently shown that Plaur, which encodes uPAR, is an early response gene in murine brain. Assumingly, diverse functions of Plaur might be attributed to hypothetical, unidentified microRNAs encoded within introns of the Plaur gene. Using a bioinformatic approach we identified novel small RNAs within the Plaur gene and named them Plaur-miR1-3p and Plaur-miR1-5p. We confirmed Plaur-dependent expression of Plaur-miR1-3p and Plaur-miR1-5p in the mouse brain and mouse neuroblastoma Neuro2a cells. Utilizing an in silico MR-microT algorithm in DianaTools we selected two target genes – Mef2d and Emx2 with the highest binding scores to small RNAs selected from identified Plaur-Pre-miR1. Furthermore, sequencing of mouse brain samples for Plaur-miR1-5p target genes revealed two more genes—Nrip3 and Snrnp200. The expression of Emx2, Mef2d, and Snrnp200 in the mouse brain and Mef2d and Snrnp200 in Neuro2a cells correlated with expression of Plaur and small RNAs—Plaur-miR1-3p and Plaur-miR1-5p. Finally, we demonstrated elevated MEF2D protein expression in the mouse brain after Plaur induction and displayed activating effects of Plaur-miR1-5p on Mef2d expression in Neuro2a cells using Luciferase reporter assay. In conclusion, we have identified Plaur-miR1-3p and Plaur-miR1-5p as novel small RNAs encoded in the Plaur gene. This finding expands the current understanding of Plaur function in brain development and functioning.

By date By citations

Citations by journals

	1
Cells	Cells, 1, 33.33% Cells 1 publication, 33.33%
Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova	Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova, 1, 33.33% Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova 1 publication, 33.33%
Neuroscience and Behavioral Physiology	Neuroscience and Behavioral Physiology, 1, 33.33% Neuroscience and Behavioral Physiology 1 publication, 33.33%
	1

Citations by publishers

	1
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 33.33% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 33.33%
Media Sphere Publishing Group	Media Sphere Publishing Group, 1, 33.33% Media Sphere Publishing Group 1 publication, 33.33%
Springer Nature	Springer Nature, 1, 33.33% Springer Nature 1 publication, 33.33%
	1

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022,2023],"ids":[0,0],"codes":[0,0],"imageUrls":["",""],"datasets":[{"label":"Citations number","data":[1,2],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":["33.33","66.67"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Cells","Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova","Neuroscience and Behavioral Physiology"],"ids":[25293,6613,1742],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/ZpQUhhOveFrtLdTt77Kuc5GTKDcUAzX6BNhjOaZC_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[33.33,33.33,33.33],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Media Sphere Publishing Group","Springer Nature"],"ids":[202,7595,8],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/ZpQUhhOveFrtLdTt77Kuc5GTKDcUAzX6BNhjOaZC_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[33.33,33.33,33.33],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Publication PDF

Metrics

Cite this

GOST |

Cite this

GOST Copy

Rysenkova K. D. et al. Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d // Frontiers in Molecular Neuroscience. 2022. Vol. 15.

GOST all authors (up to 50) Copy

Rysenkova K. D., Troyanovskiy K. E., Klimovich P. S., Bulyakova T. R., Shelomentseva E. M., Shmakova A. A., Tanygina D. Yu., Ivashkina O. I., Anokhin K. V., Karagyaur M. N., Zvereva M. I., Rubina K. A., Tkachuk V. A., Semina E. V. Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d // Frontiers in Molecular Neuroscience. 2022. Vol. 15.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.3389/fnmol.2022.865858

UR - https://doi.org/10.3389%2Ffnmol.2022.865858

TI - Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d

T2 - Frontiers in Molecular Neuroscience

AU - Rysenkova, Karina D

AU - Troyanovskiy, Konstantin E

AU - Klimovich, Polina S

AU - Bulyakova, Taisiya R

AU - Shelomentseva, Ekaterina M

AU - Shmakova, Anna A.

AU - Tanygina, Daria Yu

AU - Ivashkina, Olga I

AU - Anokhin, Konstantin V

AU - Karagyaur, Maxim N

AU - Zvereva, Maria I.

AU - Rubina, Kseniya A

AU - Tkachuk, Vsevolod A.

AU - Semina, Ekaterina V

PY - 2022

DA - 2022/07/06 00:00:00

PB - Frontiers Media S.A.

VL - 15

PMID - 35875662

SN - 1662-5099

ER -

BibTex

Cite this

BibTex Copy

@article{2022_Rysenkova,

author = {Karina D Rysenkova and Konstantin E Troyanovskiy and Polina S Klimovich and Taisiya R Bulyakova and Ekaterina M Shelomentseva and Anna A. Shmakova and Daria Yu Tanygina and Olga I Ivashkina and Konstantin V Anokhin and Maxim N Karagyaur and Maria I. Zvereva and Kseniya A Rubina and Vsevolod A. Tkachuk and Ekaterina V Semina},

title = {Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d},

journal = {Frontiers in Molecular Neuroscience},

year = {2022},

volume = {15},

publisher = {Frontiers Media S.A.},

month = {jul},

url = {https://doi.org/10.3389%2Ffnmol.2022.865858},

doi = {10.3389/fnmol.2022.865858}

}

Found error?

Publisher

Frontiers Media S.A.

Journal

Frontiers in Molecular Neuroscience

Quartile SCImago

Quartile WOS

Impact factor

4.8

ISSN

16625099 (Print)

Profiles

Zvereva, Maria Imilievna