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Frontiers in Pharmacology, volume 12

Tibetan Medicine Duoxuekang Capsule Ameliorates High-Altitude Polycythemia Accompanied by Brain Injury

Chen Ke ¹

Li Ning ²

Fan Fangfang ²

Geng Zangjia ³

Zhao Kehui ¹

Wang Jing ⁴

Zhang Yi ^{2, 5, 6}

Tang Ce ⁷

Wang Xiaobo ⁷

Meng Xianli ⁷

Hide authors affiliations Show authors affiliations: 7 affiliations

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, China |

School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, China |

School of Pharmacy, Southwest Minzu University, China |

⁴

School of Management, Chengdu University of Traditional Chinese Medicine, China |

⁵

Ethnic Medicine Academic Heritage Innovation Research Center, Chengdu University of Traditional Chinese Medicine, China |

⁶

NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Chengdu University of Traditional Chinese Medicine, China |

⁷

State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, China |

Publication type: Journal Article

Publication date: 2021-05-11

Frontiers Media S.A.

Journal: Frontiers in Pharmacology

Quartile SCImago

Quartile WOS

Impact factor: 5.6

ISSN: 16639812

DOI: 10.3389/fphar.2021.680636

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PubMed ID: 34045970

Pharmacology

Pharmacology (medical)

Abstract

Objective: Duoxuekang (DXK) capsule is an empirical prescription for Tibetan medicine in the treatment of hypobaric hypoxia (HH)-induced brain injury in the plateau. This study aimed to investigate the protective effects and underlying molecular mechanisms of DXK on HH-induced brain injury.

Methods: UPLC–Q-TOF/MS was performed for chemical composition analysis of DXK. The anti-hypoxia and anti-fatigue effects of DXK were evaluated by the normobaric hypoxia test, sodium nitrite toxicosis test, and weight-loaded swimming test in mice. Simultaneously, SD rats were used for the chronic hypobaric hypoxia (CHH) test. RBC, HGB, HCT, and the whole blood viscosity were evaluated. The activities of SOD and MDA in the brain, and EPO and LDH levels in the kidney were detected using ELISA. H&E staining was employed to observe the pathological morphology in the hippocampus and cortex of rats. Furthermore, immunofluorescence and Western blot were carried out to detect the protein expressions of Mapk10, RASGRF1, RASA3, Ras, and IGF-IR in the brain of rats. Besides, BALB/c mice were used for acute hypobaric hypoxia (AHH) test, and Western blot was employed to detect the protein expression of p-ERK/ERK, p-JNK/JNK, and p-p38/p38 in the cerebral cortex of mice.

Results: 23 different chemical compositions of DXK were identified by UPLC–Q-TOF/MS. The anti-hypoxia test verified that DXK can prolong the survival time of mice. The anti-fatigue test confirmed that DXK can prolong the swimming time of mice, decrease the level of LDH, and increase the hepatic glycogen level. Synchronously, DXK can decrease the levels of RBC, HGB, HCT, and the whole blood viscosity under the CHH condition. Besides, DXK can ameliorate CHH-induced brain injury, decrease the levels of EPO and LDH in the kidney, reduce MDA, and increase SOD in the hippocampus. Furthermore, DXK can converse HH-induced marked increase of Mapk10, RASGRF1, and RASA3, and decrease of Ras and IGF-IR. In addition, DXK can suppress the ratio of p-ERK/ERK, p-JNK/JNK, and p-p38/p38 under the HH condition.

Conclusion: Together, the cerebral protection elicited by DXK was due to the decrease of hematological index, suppressing EPO, by affecting the MAPK signaling pathway in oxidative damage, and regulating the RAS signaling pathway.

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Citations by journals

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Journal of Ethnopharmacology	Journal of Ethnopharmacology, 2, 25% Journal of Ethnopharmacology 2 publications, 25%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 12.5% International Journal of Molecular Sciences 1 publication, 12.5%
Genes	Genes, 1, 12.5% Genes 1 publication, 12.5%
Nutrients	Nutrients, 1, 12.5% Nutrients 1 publication, 12.5%
Current Opinion in Pharmacology	Current Opinion in Pharmacology, 1, 12.5% Current Opinion in Pharmacology 1 publication, 12.5%
Phytotherapy Research	Phytotherapy Research, 1, 12.5% Phytotherapy Research 1 publication, 12.5%
	1 2

Citations by publishers

	1 2 3
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 37.5% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 37.5%
Elsevier	Elsevier, 3, 37.5% Elsevier 3 publications, 37.5%
Wiley	Wiley, 1, 12.5% Wiley 1 publication, 12.5%
	1 2 3

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Chen K. et al. Tibetan Medicine Duoxuekang Capsule Ameliorates High-Altitude Polycythemia Accompanied by Brain Injury // Frontiers in Pharmacology. 2021. Vol. 12.

GOST all authors (up to 50) Copy

Chen K., Li Ning, Fan F., Geng Z., Zhao K., Wang J., Zhang Yi, Tang C., Wang X., Meng X. Tibetan Medicine Duoxuekang Capsule Ameliorates High-Altitude Polycythemia Accompanied by Brain Injury // Frontiers in Pharmacology. 2021. Vol. 12.

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RIS Copy

TY - JOUR

DO - 10.3389/fphar.2021.680636

UR - https://doi.org/10.3389%2Ffphar.2021.680636

TI - Tibetan Medicine Duoxuekang Capsule Ameliorates High-Altitude Polycythemia Accompanied by Brain Injury

T2 - Frontiers in Pharmacology

AU - Chen, Ke

AU - Li Ning

AU - Fan, Fangfang

AU - Geng, Zangjia

AU - Zhao, Kehui

AU - Wang, Jing

AU - Zhang Yi

AU - Tang, Ce

AU - Wang, Xiaobo

AU - Meng, Xianli

PY - 2021

DA - 2021/05/11 00:00:00

PB - Frontiers Media S.A.

VL - 12

PMID - 34045970

SN - 1663-9812

ER -

BibTex

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BibTex Copy

@article{2021_Chen,

author = {Ke Chen and Li Ning and Fangfang Fan and Zangjia Geng and Kehui Zhao and Jing Wang and Zhang Yi and Ce Tang and Xiaobo Wang and Xianli Meng},

title = {Tibetan Medicine Duoxuekang Capsule Ameliorates High-Altitude Polycythemia Accompanied by Brain Injury},

journal = {Frontiers in Pharmacology},

year = {2021},

volume = {12},

publisher = {Frontiers Media S.A.},

month = {may},

url = {https://doi.org/10.3389%2Ffphar.2021.680636},

doi = {10.3389/fphar.2021.680636}

}

Found error?

Publisher

Frontiers Media S.A.

Journal

Frontiers in Pharmacology

Quartile SCImago

Quartile WOS

Impact factor

5.6

ISSN

16639812 (Print)