Open Access
Scientia Pharmaceutica, volume 84, issue 2, pages 333-345
Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation
Publication type: Journal Article
Publication date: 2016-05-08
Journal:
Scientia Pharmaceutica
Quartile SCImago
Q2
Quartile WOS
—
Impact factor: 2.4
ISSN: 00368709, 22180532
PubMed ID:
27222608
Pharmaceutical Science
Abstract
A robust and rapid analysis method was developed and validated for the simultaneous assay of paclitaxel (PTX) and lapatinib (LPT) in a polymeric micelle formulation as a novel drug delivery system using high-performance liquid chromatography (HPLC). The assay was performed using the C18 MZ-Analytical Column (5 μm, 150 × 4.6 mm, OSD-3) which was protected with the C18 pre-column (5 μm, 4.0 × 4.6 mm, OSD-3). The mobile phase was composed of acetonitrile and water (70/30; V/V) with a flow rate of 0.5 mL/min and detection wavelength of 227 nm. Accuracy was reported as the relative error and was found to be less than 6.8%. The interday assay was evaluated to be 3.22% and 5.76% RSD for PTX and LPT, respectively. The intraday precision was found to be at its maximum value of 5.83% RSD. The limit of detection for both PTX and LPT was found to be 1 µg/mL by means of the newly developed method. The limit of quantitation for PTX and LPT was found to be 5 µg/mL. The calibration curves for both drugs were linear in the concentration range of 5 to 80 μg/mL. In vitro release for both drugs from the polymeric micelle was evaluated using the newly developed analysis method.
Citations by journals
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1
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Citations by publishers
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1
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3
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Elsevier
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Elsevier
3 publications, 42.86%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 14.29%
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Springer Nature
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Springer Nature
1 publication, 14.29%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
1 publication, 14.29%
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Taylor & Francis
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Taylor & Francis
1 publication, 14.29%
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1
2
3
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Saadat E. Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation // Scientia Pharmaceutica. 2016. Vol. 84. No. 2. pp. 333-345.
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Saadat E. Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation // Scientia Pharmaceutica. 2016. Vol. 84. No. 2. pp. 333-345.
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TY - JOUR
DO - 10.3797/scipharm.1507-03
UR - https://doi.org/10.3797%2Fscipharm.1507-03
TI - Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation
T2 - Scientia Pharmaceutica
AU - Saadat, Ebrahim
PY - 2016
DA - 2016/05/08 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 333-345
IS - 2
VL - 84
PMID - 27222608
SN - 0036-8709
SN - 2218-0532
ER -
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@article{2016_Saadat,
author = {Ebrahim Saadat},
title = {Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation},
journal = {Scientia Pharmaceutica},
year = {2016},
volume = {84},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {may},
url = {https://doi.org/10.3797%2Fscipharm.1507-03},
number = {2},
pages = {333--345},
doi = {10.3797/scipharm.1507-03}
}
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MLA
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Saadat, Ebrahim. “Development and Validation of a Rapid RP-HPLC-DAD Analysis Method for the Simultaneous Quantitation of Paclitaxel and Lapatinib in a Polymeric Micelle Formulation.” Scientia Pharmaceutica, vol. 84, no. 2, May. 2016, pp. 333-345. https://doi.org/10.3797%2Fscipharm.1507-03.