Open Access
Open access
eLife, volume 9

The role of Rif1 in telomere length regulation is separable from its role in origin firing

Shubin Calla B 1, 2
Greider Carol W. 1
1
 
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, United States
2
 
Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, United States
Publication typeJournal Article
Publication date2020-06-29
Journal: eLife
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor7.7
ISSN2050084X
PubMed ID:  32597753
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Neuroscience
Abstract
To examine the established link between DNA replication and telomere length, we tested whether firing of telomeric origins would cause telomere lengthening. We found that RIF1 mutants that block Protein Phosphatase 1 (PP1) binding activated telomeric origins but did not elongate telomeres. In a second approach, we found overexpression of ∆N-Dbf4 and Cdc7 increased DDK activity and activated telomeric origins, yet telomere length was unchanged. We tested a third mechanism to activate origins using the sld3-A mcm5-bob1 mutant that de-regulates the pre-replication complex, and again saw no change in telomere length. Finally, we tested whether mutations in RIF1 that cause telomere elongation would affect origin firing. We found that neither rif1-∆1322 nor rif1HOOK affected firing of telomeric origins. We conclude that telomeric origin firing does not cause telomere elongation, and the role of Rif1 in regulating origin firing is separable from its role in regulating telomere length.

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Shubin C. B., Greider C. W. The role of Rif1 in telomere length regulation is separable from its role in origin firing // eLife. 2020. Vol. 9.
GOST all authors (up to 50) Copy
Shubin C. B., Greider C. W. The role of Rif1 in telomere length regulation is separable from its role in origin firing // eLife. 2020. Vol. 9.
RIS |
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RIS Copy
TY - JOUR
DO - 10.7554/eLife.58066
UR - https://doi.org/10.7554%2FeLife.58066
TI - The role of Rif1 in telomere length regulation is separable from its role in origin firing
T2 - eLife
AU - Shubin, Calla B
AU - Greider, Carol W.
PY - 2020
DA - 2020/06/29 00:00:00
PB - eLife Sciences Publications
VL - 9
PMID - 32597753
SN - 2050-084X
ER -
BibTex
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BibTex Copy
@article{2020_Shubin
author = {Calla B Shubin and Carol W. Greider},
title = {The role of Rif1 in telomere length regulation is separable from its role in origin firing},
journal = {eLife},
year = {2020},
volume = {9},
publisher = {eLife Sciences Publications},
month = {jun},
url = {https://doi.org/10.7554%2FeLife.58066},
doi = {10.7554/eLife.58066}
}
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