Open Access
eLife, volume 10
The final step of 40S ribosomal subunit maturation is controlled by a dual key lock
Plassart Laura
1
,
Shayan Ramtin
1
,
Montellese Christian
2
,
Rinaldi Dana
1
,
Larburu Natacha
1
,
Pichereaux Carole
3
,
Froment Carine
3
,
Lebaron Simon
1
,
Odonohue Marie Françoise
1
,
Kutay U.
2
,
Marcoux Julien
3
,
Gleizes Pierre-Emmanuel
1
,
Plisson-Chastang Celia
1
1
Molecular, Cellular and Developmental Biology Department (MCD), Centre de Biologie Integrative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France
|
3
Institut de Pharmacologie et Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France
|
Publication type: Journal Article
Publication date: 2021-04-28
PubMed ID:
33908345
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Neuroscience
Abstract
Preventing premature interaction of preribosomes with the translation apparatus is essential to translation accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3’ end, is safeguarded by protein DIM2, which both interacts with the endoribonuclease NOB1 and masks the rRNA cleavage site. To elucidate the control mechanism that unlocks NOB1 activity, we performed cryo-EM analysis of late human pre-40S particles purified using a catalytically-inactive form of ATPase RIO1. These structures, together with in vivo and in vitro functional analyses, support a model in which ATP-loaded RIO1 cooperates with ribosomal protein RPS26/eS26 to displace DIM2 from the 18S rRNA 3’ end, thereby triggering final cleavage by NOB1; release of ADP then leads to RIO1 dissociation from the 40S subunit. This dual key lock mechanism requiring RIO1 and RPS26 guarantees the precise timing of pre-40S particle conversion into translation-competent ribosomal subunits.
Citations by journals
|
1
2
|
|
|
Nucleic Acids Research
|
Nucleic Acids Research
2 publications, 9.52%
|
|
RNA
|
RNA
2 publications, 9.52%
|
|
Acta Naturae
|
Acta Naturae, 1, 4.76%
Acta Naturae
1 publication, 4.76%
|
|
Journal of Cell Biology
|
Journal of Cell Biology
1 publication, 4.76%
|
|
Genes
|
Genes
1 publication, 4.76%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 4.76%
|
|
PLoS ONE
|
PLoS ONE
1 publication, 4.76%
|
|
Current Opinion in Structural Biology
|
Current Opinion in Structural Biology
1 publication, 4.76%
|
|
Nature Communications
|
Nature Communications
1 publication, 4.76%
|
|
Wiley interdisciplinary reviews. RNA
|
Wiley interdisciplinary reviews. RNA
1 publication, 4.76%
|
|
RNA Biology
|
RNA Biology
1 publication, 4.76%
|
|
EMBO Journal
|
EMBO Journal
1 publication, 4.76%
|
|
eLife
|
eLife
1 publication, 4.76%
|
|
Blood
|
Blood
1 publication, 4.76%
|
|
1
2
|
Citations by publishers
|
1
2
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 9.52%
|
|
Oxford University Press
|
Oxford University Press
2 publications, 9.52%
|
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
2 publications, 9.52%
|
|
Acta Naturae Ltd
|
Acta Naturae Ltd, 1, 4.76%
Acta Naturae Ltd
1 publication, 4.76%
|
|
Rockefeller University Press
|
Rockefeller University Press
1 publication, 4.76%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
1 publication, 4.76%
|
|
Elsevier
|
Elsevier
1 publication, 4.76%
|
|
Springer Nature
|
Springer Nature
1 publication, 4.76%
|
|
Wiley
|
Wiley
1 publication, 4.76%
|
|
Taylor & Francis
|
Taylor & Francis
1 publication, 4.76%
|
|
EMBO press
|
EMBO press
1 publication, 4.76%
|
|
eLife Sciences Publications
|
eLife Sciences Publications
1 publication, 4.76%
|
|
American Society of Hematology
|
American Society of Hematology
1 publication, 4.76%
|
|
1
2
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[5,9,6,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["23.81","42.86","28.57","4.76"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nucleic Acids Research","RNA","Acta Naturae","Journal of Cell Biology","Genes","International Journal of Molecular Sciences","PLoS ONE","Current Opinion in Structural Biology","Nature Communications","Wiley interdisciplinary reviews. RNA","RNA Biology","EMBO Journal","eLife","Blood"],"ids":[23904,12892,7401,867,8879,14627,24963,13702,3231,8123,7613,16016,24138,6594],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/QMhhcrIUiVtFnND3WWZ7hIbu6GYeoRoA2zoVsMEX_medium.webp"],"datasets":[{"label":"","data":[2,2,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[9.52,9.52,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Oxford University Press","Cold Spring Harbor Laboratory","Acta Naturae Ltd","Rockefeller University Press","Public Library of Science (PLoS)","Elsevier","Springer Nature","Wiley","Taylor & Francis","EMBO press","eLife Sciences Publications","American Society of Hematology"],"ids":[202,19,6909,7905,22,344,17,8,11,18,9415,6627,2252],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/QMhhcrIUiVtFnND3WWZ7hIbu6GYeoRoA2zoVsMEX_medium.webp"],"datasets":[{"label":"","data":[2,2,2,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[9.52,9.52,9.52,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76,4.76],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Plassart L. et al. The final step of 40S ribosomal subunit maturation is controlled by a dual key lock // eLife. 2021. Vol. 10.
GOST all authors (up to 50)
Copy
Plassart L., Shayan R., Montellese C., Rinaldi D., Larburu N., Pichereaux C., Froment C., Lebaron S., Odonohue M. F., Kutay U., Marcoux J., Gleizes P., Plisson-Chastang C. The final step of 40S ribosomal subunit maturation is controlled by a dual key lock // eLife. 2021. Vol. 10.
Cite this
RIS
Copy
TY - JOUR
DO - 10.7554/eLife.61254
UR - https://doi.org/10.7554%2FeLife.61254
TI - The final step of 40S ribosomal subunit maturation is controlled by a dual key lock
T2 - eLife
AU - Plassart, Laura
AU - Shayan, Ramtin
AU - Montellese, Christian
AU - Rinaldi, Dana
AU - Larburu, Natacha
AU - Pichereaux, Carole
AU - Froment, Carine
AU - Lebaron, Simon
AU - Odonohue, Marie Françoise
AU - Kutay, U.
AU - Marcoux, Julien
AU - Gleizes, Pierre-Emmanuel
AU - Plisson-Chastang, Celia
PY - 2021
DA - 2021/04/28 00:00:00
PB - eLife Sciences Publications
VL - 10
PMID - 33908345
SN - 2050-084X
ER -
Cite this
BibTex
Copy
@article{2021_Plassart,
author = {Laura Plassart and Ramtin Shayan and Christian Montellese and Dana Rinaldi and Natacha Larburu and Carole Pichereaux and Carine Froment and Simon Lebaron and Marie Françoise Odonohue and U. Kutay and Julien Marcoux and Pierre-Emmanuel Gleizes and Celia Plisson-Chastang},
title = {The final step of 40S ribosomal subunit maturation is controlled by a dual key lock},
journal = {eLife},
year = {2021},
volume = {10},
publisher = {eLife Sciences Publications},
month = {apr},
url = {https://doi.org/10.7554%2FeLife.61254},
doi = {10.7554/eLife.61254}
}