Open Access
Open access
eLife, том 11

Telomere length regulation by Rif1 protein from Hansenula polymorpha

Тип документаJournal Article
Дата публикации2022-02-07
ИздательeLife Sciences Publications
Название журналаeLife
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 20218.71
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Neuroscience
Краткое описание
Rif1 is a large multifaceted protein involved in various processes of DNA metabolism – from telomere length regulation and replication to double-strand break repair. The mechanistic details of its action, however, are often poorly understood. Here, we report functional characterization of the Rif1 homologue from methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. We show that, similar to other yeast species, H. polymorpha Rif1 suppresses telomerase-dependent telomere elongation. We uncover two novel modes of Rif1 recruitment at H. polymorpha telomeres: via direct DNA binding and through the association with the Ku heterodimer. Both of these modes (at least partially) require the intrinsically disordered N-terminal extension – a region of the protein present exclusively in yeast species. We also demonstrate that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha.
Пристатейные ссылки: 84
Alternative paths to telomere elongation.
Lee J.J., Lee J., Lee H.
Q1 Seminars in Cell and Developmental Biology 2021 цитирований: 6
Rif1 regulates telomere length through conserved HEAT repeats
Shubin C.B., Mayangsari R., Swett A.D., Greider C.W.
Q1 Nucleic Acids Research 2021 цитирований: 7
Open Access
Open access
Shaping human telomeres: from shelterin and CST complexes to telomeric chromatin organization
Lim C.J., Cech T.R.
Q1 Nature Reviews Molecular Cell Biology 2021 цитирований: 62
Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase
Shepelev N.M., Mariasina S.S., Mantsyzov A.B., Malyavko A.N., Efimov S.V., Petrova O.A., Rodina E.V., Zvereva M.I., Dontsova O.A., Polshakov V.I.
Q1 Scientific Reports 2020 цитирований: 3
Open Access
Open access
G-quadruplex binding protein Rif1, a key regulator of replication timing.
Alavi S., Ghadiri H., Dabirmanesh B., Moriyama K., Khajeh K., Masai H.
Q1 Journal of Biochemistry 2020 цитирований: 12
Open Access
Open access
The role of Rif1 in telomere length regulation is separable from its role in origin firing
Shubin C.B., Greider C.W.
Q1 eLife 2020 цитирований: 11
Open Access
Open access
The telomeric Cdc13 protein from yeast hansenula polymorpha
Malyavko A.N., Dontsova O.A.
Q3 Acta Naturae 2020 цитирований: 2
Open Access
Open access
Fork pausing complex engages topoisomerases at the replisome.
Shyian M., Albert B., Zupan A.M., Ivanitsa V., Charbonnet G., Dilg D., Shore D.
Q1 Genes and Development 2019 цитирований: 16
Functional duplication of Rap1 in methylotrophic yeasts
Malyavko A.N., Petrova O.A., Zvereva M.I., Dontsova O.A.
Q1 Scientific Reports 2019 цитирований: 3
Open Access
Open access
Plugged into the Ku-DNA hub: The NHEJ network.
Frit P., Ropars V., Modesti M., Charbonnier J.B., Calsou P.
Q1 Progress in Biophysics and Molecular Biology 2019 цитирований: 37
The telomeric Cdc13–Stn1–Ten1 complex regulates RNA polymerase II transcription
Calvo O., Grandin N., Jordán-Pla A., Miñambres E., González-Polo N., Pérez-Ortín J.E., Charbonneau M.
Q1 Nucleic Acids Research 2019 цитирований: 6
Open Access
Open access
Telomeres and telomerase: three decades of progress
Shay J.W., Wright W.E.
Q1 Nature Reviews Genetics 2019 цитирований: 344
Shelterin-Mediated Telomere Protection
de Lange T.
Q1 Annual Review of Genetics 2018 цитирований: 376
ChECing out Rif1 action in freely cycling cells
Hafner L., Shore D., Mattarocci S.
Q1 Current Genetics 2018 цитирований: 5
Fine tuning the level of the Cdc13 telomere-capping protein for maximal chromosome stability performance
Mersaoui S.Y., Wellinger R.J.
Q1 Current Genetics 2018 цитирований: 13


1. Malyavko A. N. и др. Telomere length regulation by Rif1 protein from Hansenula polymorpha // eLife. 2022. Т. 11.


DO - 10.7554/elife.75010

UR -

TI - Telomere length regulation by Rif1 protein from Hansenula polymorpha

T2 - eLife

AU - Malyavko, Alexander N

AU - Petrova, Olga A

AU - Zvereva, Maria I

AU - Polshakov, Vladimir I

AU - Dontsova, Olga A

PY - 2022

DA - 2022/02/07

PB - eLife Sciences Publications, Ltd

VL - 11

SN - 2050-084X

ER -

BibTex |


doi = {10.7554/elife.75010},

url = {},

year = 2022,

month = {feb},

publisher = {{eLife} Sciences Publications, Ltd},

volume = {11},

author = {Alexander N Malyavko and Olga A Petrova and Maria I Zvereva and Vladimir I Polshakov and Olga A Dontsova},

title = {Telomere length regulation by Rif1 protein from Hansenula polymorpha},

journal = {{eLife}}


Malyavko, Alexander N., et al. “Telomere Length Regulation by Rif1 Protein from Hansenula Polymorpha.” ELife, vol. 11, Feb. 2022. CLOCKSS, Crossref,