Open Access
eLife, volume 11
Telomere length regulation by Rif1 protein from Hansenula polymorpha
Publication type: Journal Article
Publication date: 2022-02-07
PubMed ID:
35129114
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Neuroscience
Abstract
Rif1 is a large multifaceted protein involved in various processes of DNA metabolism – from telomere length regulation and replication to double-strand break repair. The mechanistic details of its action, however, are often poorly understood. Here, we report functional characterization of the Rif1 homologue from methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. We show that, similar to other yeast species, H. polymorpha Rif1 suppresses telomerase-dependent telomere elongation. We uncover two novel modes of Rif1 recruitment at H. polymorpha telomeres: via direct DNA binding and through the association with the Ku heterodimer. Both of these modes (at least partially) require the intrinsically disordered N-terminal extension – a region of the protein present exclusively in yeast species. We also demonstrate that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha.
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 100%
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Malyavko A. N. et al. Telomere length regulation by Rif1 protein from Hansenula polymorpha // eLife. 2022. Vol. 11.
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Malyavko A. N., Petrova O. A., Zvereva M. I., Polshakov V. I., Dontsova O. A. Telomere length regulation by Rif1 protein from Hansenula polymorpha // eLife. 2022. Vol. 11.
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TY - JOUR
DO - 10.7554/eLife.75010
UR - https://doi.org/10.7554%2FeLife.75010
TI - Telomere length regulation by Rif1 protein from Hansenula polymorpha
T2 - eLife
AU - Malyavko, Alexander N
AU - Petrova, Olga A
AU - Zvereva, Maria I.
AU - Polshakov, Vladimir I.
AU - Dontsova, Olga A.
PY - 2022
DA - 2022/02/07 00:00:00
PB - eLife Sciences Publications
VL - 11
PMID - 35129114
SN - 2050-084X
ER -
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@article{2022_Malyavko,
author = {Alexander N Malyavko and Olga A Petrova and Maria I. Zvereva and Vladimir I. Polshakov and Olga A. Dontsova},
title = {Telomere length regulation by Rif1 protein from Hansenula polymorpha},
journal = {eLife},
year = {2022},
volume = {11},
publisher = {eLife Sciences Publications},
month = {feb},
url = {https://doi.org/10.7554%2FeLife.75010},
doi = {10.7554/eLife.75010}
}