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PeerJ, volume 6, pages e5832

Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia

Voytsekhovskaya Irina V. ¹

Axenov-Gribanov Denis V. ^{1, 2}

Murzina Svetlana A. ³

Pekkoeva Svetlana N. ³

Protasov Eugeniy S. ¹

Gamaiunov Stanislav V. ²

Timofeyev Maxim A. ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

Irkutsk State University, Irkutsk, Russia |

Baikal Research Centre, Irkutsk, Russia |

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Petrozavodsk, Karelia, Russia

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Karelian Research Centre of the Russian Academy of Sciences

Publication type: Journal Article

Publication date: 2018-10-25

PeerJ

Journal: PeerJ

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Impact factor: 2.7

ISSN: 21678359

DOI: 10.7717/peerj.5832

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PubMed ID: 30386707

General Biochemistry, Genetics and Molecular Biology

General Medicine

General Agricultural and Biological Sciences

General Neuroscience

Abstract

Extreme and unusual ecosystems such as isolated ancient caves are considered as potential tools for the discovery of novel natural products with biological activities. Actinobacteria that inhabit these unusual ecosystems are examined as a promising source for the development of new drugs. In this study we focused on the preliminary estimation of fatty acid composition and antibacterial properties of culturable actinobacteria isolated from water surface of underground lakes located in Badzheyskaya and Okhotnichya caves in Siberia. Here we present isolation of 17 strains of actinobacteria that belong to theStreptomyces,NocardiaandNocardiopsisgenera. Using assays for antibacterial and antifungal activities, we found that a number of strains belonging to the genusStreptomycesisolated from Badzheyskaya cave demonstrated inhibition activity against bacteria and fungi. It was shown that representatives of the generaNocardiaandNocardiopsisisolated from Okhotnichya cave did not demonstrate any tested antibiotic properties. However, despite the lack of antimicrobial and fungicidal activity ofNocardiaextracts, those strains are specific in terms of their fatty acid spectrum. When assessing fatty acid profile, we found that polyunsaturated fatty acids were quantitatively dominant in extracts ofNocardiasp. andStreptomycessp. grown in different media. Saturated fatty acids were the second most abundant type in the fatty acid profile. It was due to palmitic acid. Also, a few monounsaturated fatty acids were detected. The obtained materials can become a basis for development of approaches to use bacteria isolated from caves as a biological sources of bioactive compounds to create medical and veterinary drugs.

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Citations by journals

	1 2
Microorganisms	Microorganisms, 2, 28.57% Microorganisms 2 publications, 28.57%
3 Biotech	3 Biotech, 1, 14.29% 3 Biotech 1 publication, 14.29%
Applied Sciences (Switzerland)	Applied Sciences (Switzerland), 1, 14.29% Applied Sciences (Switzerland) 1 publication, 14.29%
Frontiers in Microbiology	Frontiers in Microbiology, 1, 14.29% Frontiers in Microbiology 1 publication, 14.29%
Journal of Analytical and Applied Pyrolysis	Journal of Analytical and Applied Pyrolysis, 1, 14.29% Journal of Analytical and Applied Pyrolysis 1 publication, 14.29%
Biotechnology and Applied Biochemistry	Biotechnology and Applied Biochemistry, 1, 14.29% Biotechnology and Applied Biochemistry 1 publication, 14.29%
	1 2

Citations by publishers

	1 2 3
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 42.86% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 42.86%
Springer Nature	Springer Nature, 1, 14.29% Springer Nature 1 publication, 14.29%
Frontiers Media S.A.	Frontiers Media S.A., 1, 14.29% Frontiers Media S.A. 1 publication, 14.29%
Elsevier	Elsevier, 1, 14.29% Elsevier 1 publication, 14.29%
Wiley	Wiley, 1, 14.29% Wiley 1 publication, 14.29%
	1 2 3

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Voytsekhovskaya I. V. et al. Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia // PeerJ. 2018. Vol. 6. p. e5832.

GOST all authors (up to 50) Copy

Voytsekhovskaya I. V., Axenov-Gribanov D. V., Murzina S. A., Pekkoeva S. N., Protasov E. S., Gamaiunov S. V., Timofeyev M. A. Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia // PeerJ. 2018. Vol. 6. p. e5832.

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RIS Copy

TY - JOUR

DO - 10.7717/peerj.5832

UR - https://doi.org/10.7717%2Fpeerj.5832

TI - Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia

T2 - PeerJ

AU - Voytsekhovskaya, Irina V.

AU - Axenov-Gribanov, Denis V.

AU - Murzina, Svetlana A.

AU - Pekkoeva, Svetlana N.

AU - Protasov, Eugeniy S.

AU - Gamaiunov, Stanislav V.

AU - Timofeyev, Maxim A.

PY - 2018

DA - 2018/10/25 00:00:00

PB - PeerJ

SP - e5832

VL - 6

PMID - 30386707

SN - 2167-8359

ER -

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@article{2018_Voytsekhovskaya,

author = {Irina V. Voytsekhovskaya and Denis V. Axenov-Gribanov and Svetlana A. Murzina and Svetlana N. Pekkoeva and Eugeniy S. Protasov and Stanislav V. Gamaiunov and Maxim A. Timofeyev},

title = {Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia},

journal = {PeerJ},

year = {2018},

volume = {6},

publisher = {PeerJ},

month = {oct},

url = {https://doi.org/10.7717%2Fpeerj.5832},

pages = {e5832},

doi = {10.7717/peerj.5832}

}

Found error?

Publisher

PeerJ

Journal

PeerJ

Quartile SCImago

Quartile WOS

Impact factor

2.7

ISSN

21678359 (Print)

Labs

Laboratory of Pharmaceutical Biotechnology of the ISU

Profiles

Aksenov-Gribanov, Denis Viktorovich