Open Access
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Journal of Cell Biology, volume 185, issue 1, pages 87-100

Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template

Hu Yan 1
Kireev Igor 1
Plutz Matt 1
Ashourian Nazanin 1
Belmont Andrew S. 1
1
 
Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801
Publication typeJournal Article
Publication date2009-04-06
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor7.8
ISSN00219525, 15408140
Cell Biology
Abstract

The structure of interphase chromosomes, and in particular the changes in large-scale chromatin structure accompanying transcriptional activation, remain poorly characterized. Here we use light microscopy and in vivo immunogold labeling to directly visualize the interphase chromosome conformation of 1–2 Mbp chromatin domains formed by multi-copy BAC transgenes containing 130–220 kb of genomic DNA surrounding the DHFR, Hsp70, or MT gene loci. We demonstrate near-endogenous transcription levels in the context of large-scale chromatin fibers compacted nonuniformly well above the 30-nm chromatin fiber. An approximately 1.5–3-fold extension of these large-scale chromatin fibers accompanies transcriptional induction and active genes remain mobile. Heat shock–induced Hsp70 transgenes associate with the exterior of nuclear speckles, with Hsp70 transcripts accumulating within the speckle. Live-cell imaging reveals distinct dynamic events, with Hsp70 transgenes associating with adjacent speckles, nucleating new speckles, or moving to preexisting speckles. Our results call for reexamination of classical models of interphase chromosome organization.

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GOST |
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GOST Copy
Hu Y. et al. Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template // Journal of Cell Biology. 2009. Vol. 185. No. 1. pp. 87-100.
GOST all authors (up to 50) Copy
Hu Y., Kireev I., Plutz M., Ashourian N., Belmont A. S. Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template // Journal of Cell Biology. 2009. Vol. 185. No. 1. pp. 87-100.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1083/JCB.200809196
UR - https://doi.org/10.1083%2FJCB.200809196
TI - Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template
T2 - Journal of Cell Biology
AU - Plutz, Matt
AU - Ashourian, Nazanin
AU - Hu, Yan
AU - Kireev, Igor
AU - Belmont, Andrew S.
PY - 2009
DA - 2009/04/06 00:00:00
PB - Rockefeller University Press
SP - 87-100
IS - 1
VL - 185
PMID - 19349581
SN - 0021-9525
SN - 1540-8140
ER -
BibTex |
Cite this
BibTex Copy
@article{2009_Hu
author = {Matt Plutz and Nazanin Ashourian and Yan Hu and Igor Kireev and Andrew S. Belmont},
title = {Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template},
journal = {Journal of Cell Biology},
year = {2009},
volume = {185},
publisher = {Rockefeller University Press},
month = {apr},
url = {https://doi.org/10.1083%2FJCB.200809196},
number = {1},
pages = {87--100},
doi = {10.1083/JCB.200809196}
}
MLA
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MLA Copy
Hu, Yan, et al. “Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template.” Journal of Cell Biology, vol. 185, no. 1, Apr. 2009, pp. 87-100. https://doi.org/10.1083%2FJCB.200809196.
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