Nematology, volume 23, issue 4, pages 451-466
Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach
1
Faculty of Agriculture, Agriculture and Forestry University, Rampur, Chitwan, Nepal
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2
Entomology and Nematology Department, Institute of Food and Agriculture Science, University of Florida, Gainesville, FL 32611-0620, USA
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3
CABI, Egham, Surrey TW20 9TY, UK
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4
e-nema GmbH, Klausdorfer Str., 28-36, D-24223 Schwentinental, Germany
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6
Plant Pest Diagnostic Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832, USA
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Publication type: Journal Article
Publication date: 2020-01-01
Agronomy and Crop Science
Ecology, Evolution, Behavior and Systematics
Abstract
Summary
Presently, the genus Heterorhabditis contains 16 valid entomopathogenic nematode species. In this study we used samples from 11 species: H. amazonensis, H. bacteriophora, H. baujardi, H. beicherriana, H. downesi, H. floridensis, H. georgiana, H. indica, H. megidis, H. noenieputensis, and H. zealandica to amplify and sequence five gene fragments: the D2-D3 expansion segments of 28S rRNA, ITS rRNA, COI mtDNA genes and unc-87 and cmd-1 genes encoding thin filament (F-actin)-associated protein and calmodulin, respectively. Fifty new sequences for 11 species were generated. More than 980 sequences of five genes were analysed. Phylogenetic and sequence analysis of these genes using Bayesian inference, maximum likelihood and statistical parsimony confirmed a division of the genus into three clades (groups): ‘Indica’, ‘Bacteriophora’ and ‘Megidis’. The analysis of gene sequences downloaded from GenBank and identified as Heterorhabditis revealed many cases of species misidentifications and presence of reading mistakes in some sequences. Synonymisation of H. somsookae with H. baujardi, H. gerrardi, H. pakistanensis with H. indica, and H. sonorensis with H. taysearae, are confirmed by sequence and phylogenetic analysis. The ITS rRNA and COI genes could be considered as informative markers for species identification, barcoding and phylogeographical studies of Heterorhabditis.
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- We do not take into account publications that without a DOI.
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Dhakal M. et al. Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach // Nematology. 2020. Vol. 23. No. 4. pp. 451-466.
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Dhakal M., Nguyen K. B., Hunt D. J., Ehlers R., Spiridonov S. E., Subbotin S. A. Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach // Nematology. 2020. Vol. 23. No. 4. pp. 451-466.
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TY - JOUR
DO - 10.1163/15685411-bja10052
UR - https://doi.org/10.1163%2F15685411-bja10052
TI - Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach
T2 - Nematology
AU - Dhakal, Manoj
AU - Ehlers, Ralf-Udo
AU - Nguyen, Khuong B
AU - Hunt, David J
AU - Spiridonov, Sergei E
AU - Subbotin, Sergei A.
PY - 2020
DA - 2020/01/01 00:00:00
PB - Brill
SP - 451-466
IS - 4
VL - 23
SN - 1388-5545
SN - 1568-5411
ER -
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@article{2020_Dhakal,
author = {Manoj Dhakal and Ralf-Udo Ehlers and Khuong B Nguyen and David J Hunt and Sergei E Spiridonov and Sergei A. Subbotin},
title = {Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach},
journal = {Nematology},
year = {2020},
volume = {23},
publisher = {Brill},
month = {jan},
url = {https://doi.org/10.1163%2F15685411-bja10052},
number = {4},
pages = {451--466},
doi = {10.1163/15685411-bja10052}
}
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Dhakal, Manoj, et al. “Molecular identification, phylogeny and phylogeography of the entomopathogenic nematodes of the genus Heterorhabditis Poinar, 1976: A multigene approach.” Nematology, vol. 23, no. 4, Jan. 2020, pp. 451-466. https://doi.org/10.1163%2F15685411-bja10052.
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