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Open access

In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

Kelmanson I.V., Shokhina A.G., Kotova D.A., Pochechuev M.S., Ivanova A.D., Kostyuk A.I., Panova A.S., Borodinova A.A., Solotenkov M.A., Stepanov E.A., Raevskii R.I., Moshchenko A.A., Pak V.V., Ermakova Y.G., van Belle G.J., Tarabykin V., Balaban P.M., Fedotov I.V., Fedotov A.B., Conrad M., Bogeski I., Katschinski D.M., Doeppner T.R., Bähr M., Zheltikov A.M., Belousov V.V., Bilan D.S.
Тип документаJournal Article
Дата публикации2021-12-01
Название журналаRedox Biology
  • Organic Chemistry
  • Biochemistry
  • Clinical Biochemistry
Краткое описание
Ischemic cerebral stroke is one of the leading causes of death and disability in humans. However, molecular processes underlying the development of this pathology remain poorly understood. There are major gaps in our understanding of metabolic changes that occur in the brain tissue during the early stages of ischemia and reperfusion. In particular, it is generally accepted that both ischemia (I) and reperfusion (R) generate reactive oxygen species (ROS) that cause oxidative stress which is one of the main drivers of the pathology, although ROS generation during I/R was never demonstrated in vivo due to the lack of suitable methods. In the present study, we record for the first time the dynamics of intracellular pH and H2O2 during I/R in cultured neurons and during experimental stroke in rats using the latest generation of genetically encoded biosensors SypHer3s and HyPer7. We detect a buildup of powerful acidosis in the brain tissue that overlaps with the ischemic core from the first seconds of pathogenesis. At the same time, no significant H2O2 generation was found in the acute phase of ischemia/reperfusion. HyPer7 oxidation in the brain was detected only 24 h later. Comparison of in vivo experiments with studies on cultured neurons under I/R demonstrates that the dynamics of metabolic processes in these models significantly differ, suggesting that a cell culture is a poor predictor of metabolic events in vivo.
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1. Kelmanson I.V. и др. In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model // Redox Biology. 2021. Т. 48. С. 102178.


DO - 10.1016/j.redox.2021.102178

UR -

TI - In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

T2 - Redox Biology

AU - Kelmanson, Ilya V.

AU - Shokhina, Arina G.

AU - Kotova, Daria A.

AU - Pochechuev, Matvei S.

AU - Ivanova, Alexandra D.

AU - Kostyuk, Alexander I.

AU - Panova, Anastasiya S.

AU - Borodinova, Anastasia A.

AU - Solotenkov, Maxim A.

AU - Stepanov, Evgeny A.

AU - Raevskii, Roman I.

AU - Moshchenko, Aleksandr A.

AU - Pak, Valeriy V.

AU - Ermakova, Yulia G.

AU - van Belle, Gijsbert J.C.

AU - Tarabykin, Viktor

AU - Balaban, Pavel M.

AU - Fedotov, Ilya V.

AU - Fedotov, Andrei B.

AU - Conrad, Marcus

AU - Bogeski, Ivan

AU - Katschinski, Dörthe M.

AU - Doeppner, Thorsten R.

AU - Bähr, Mathias

AU - Zheltikov, Aleksei M.

AU - Belousov, Vsevolod V.

AU - Bilan, Dmitry S.

PY - 2021

DA - 2021/12

PB - Elsevier BV

SP - 102178

VL - 48

SN - 2213-2317

ER -

BibTex |


doi = {10.1016/j.redox.2021.102178},

url = {},

year = 2021,

month = {dec},

publisher = {Elsevier {BV}},

volume = {48},

pages = {102178},

author = {Ilya V. Kelmanson and Arina G. Shokhina and Daria A. Kotova and Matvei S. Pochechuev and Alexandra D. Ivanova and Alexander I. Kostyuk and Anastasiya S. Panova and Anastasia A. Borodinova and Maxim A. Solotenkov and Evgeny A. Stepanov and Roman I. Raevskii and Aleksandr A. Moshchenko and Valeriy V. Pak and Yulia G. Ermakova and Gijsbert J.C. van Belle and Viktor Tarabykin and Pavel M. Balaban and Ilya V. Fedotov and Andrei B. Fedotov and Marcus Conrad and Ivan Bogeski and Dörthe M. Katschinski and Thorsten R. Doeppner and Mathias Bähr and Aleksei M. Zheltikov and Vsevolod V. Belousov and Dmitry S. Bilan},

title = {In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model},

journal = {Redox Biology}


Kelmanson, Ilya V. et al. “In Vivo Dynamics of Acidosis and Oxidative Stress in the Acute Phase of an Ischemic Stroke in a Rodent Model.” Redox Biology 48 (2021): 102178. Crossref. Web.