Open Access
Open access

In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

Kelmanson I.V., Shokhina A.G., Kotova D.A., Pochechuev M.S., Ivanova A.D., Kostyuk A.I., Panova A.S., Borodinova A.A., Solotenkov M.A., Stepanov E.A., Raevskii R.I., Moshchenko A.A., Pak V.V., Ermakova Y.G., van Belle G.J., Tarabykin V., Balaban P.M., Fedotov I.V., Fedotov A.B., Conrad M., Bogeski I., Katschinski D.M., Doeppner T.R., Bähr M., Zheltikov A.M., Belousov V.V., Bilan D.S.
Тип документаJournal Article
Дата публикации2021-12-01
Название журналаRedox Biology
ИздательElsevier
КвартильQ1
ISSN22132317
  • Organic Chemistry
  • Biochemistry
  • Clinical Biochemistry
Краткое описание
Ischemic cerebral stroke is one of the leading causes of death and disability in humans. However, molecular processes underlying the development of this pathology remain poorly understood. There are major gaps in our understanding of metabolic changes that occur in the brain tissue during the early stages of ischemia and reperfusion. In particular, it is generally accepted that both ischemia (I) and reperfusion (R) generate reactive oxygen species (ROS) that cause oxidative stress which is one of the main drivers of the pathology, although ROS generation during I/R was never demonstrated in vivo due to the lack of suitable methods. In the present study, we record for the first time the dynamics of intracellular pH and H2O2 during I/R in cultured neurons and during experimental stroke in rats using the latest generation of genetically encoded biosensors SypHer3s and HyPer7. We detect a buildup of powerful acidosis in the brain tissue that overlaps with the ischemic core from the first seconds of pathogenesis. At the same time, no significant H2O2 generation was found in the acute phase of ischemia/reperfusion. HyPer7 oxidation in the brain was detected only 24 h later. Comparison of in vivo experiments with studies on cultured neurons under I/R demonstrates that the dynamics of metabolic processes in these models significantly differ, suggesting that a cell culture is a poor predictor of metabolic events in vivo.
Пристатейные ссылки: 74
Цитируется в публикациях: 4
Pathophysiology and Treatment of Stroke: Present Status and Future Perspectives
Kuriakose D., Xiao Z.
Q1 International Journal of Molecular Sciences 2020 цитирований: 33
Open Access
Open access
Optogenetic translocation of protons out of penumbral neurons is protective in a rodent model of focal cerebral ischemia
Bo B., Li Y., Li W., Wang Y., Tong S.
Q1 Brain Stimulation 2020 цитирований: 3
Open Access
Open access
Ischemia-Triggered Glutamate Excitotoxicity From the Perspective of Glial Cells
Belov Kirdajova D., Kriska J., Tureckova J., Anderova M.
Q2 Frontiers in Cellular Neuroscience 2020 цитирований: 53
Open Access
Open access
Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function
Pak V.V., Ezeriņa D., Lyublinskaya O.G., Pedre B., Tyurin-Kuzmin P.A., Mishina N.M., Thauvin M., Young D., Wahni K., Martínez Gache S.A., Demidovich A.D., Ermakova Y.G., Maslova Y.D., Shokhina A.G., Eroglu E., et. al.
Q1 Cell Metabolism 2020 цитирований: 66
Demarcation of Sepsis-Induced Peripheral and Central Acidosis with pH (Low) Insertion Cycle Peptide
Henry K.E., Chaney A.M., Nagle V.L., Cropper H.C., Mozaffari S., Slaybaugh G., Parang K., Andreev O.A., Reshetnyak Y.K., James M.L., Lewis J.S.
Q1 Journal of Nuclear Medicine 2020 цитирований: 3
NINscope, a versatile miniscope for multi-region circuit investigations
de Groot A., van den Boom B.J., van Genderen R.M., Coppens J., van Veldhuijzen J., Bos J., Hoedemaker H., Negrello M., Willuhn I., De Zeeuw C.I., Hoogland T.M.
Q1 eLife 2020 цитирований: 35
Open Access
Open access
Two‐ and three‐photon absorption cross‐section characterization for high‐brightness, cell‐specific multiphoton fluorescence brain imaging
Lanin A.A., Chebotarev A.S., Pochechuev M.S., Kelmanson I.V., Kotova D.A., Bilan D.S., Ermakova Y.G., Fedotov A.B., Ivanov A.A., Belousov V.V., Zheltikov A.M.
Q1 Journal of Biophotonics 2020 цитирований: 9
High-density multi-fiber photometry for studying large-scale brain circuit dynamics
Sych Y., Chernysheva M., Sumanovski L.T., Helmchen F.
Q1 Nature Methods 2019 цитирований: 60
O2 affects mitochondrial functionality ex vivo
Nanadikar M.S., Vergel Leon A.M., Borowik S., Hillemann A., Zieseniss A., Belousov V.V., Bogeski I., Rehling P., Dudek J., Katschinski D.M.
Q1 Redox Biology 2019 цитирований: 13
Open Access
Open access
Calcium-dependent blood-brain barrier breakdown by NOX5 limits postreperfusion benefit in stroke
Casas A.I., Kleikers P.W., Geuss E., Langhauser F., Adler T., Busch D.H., Gailus-Durner V., de Angelis M.H., Egea J., Lopez M.G., Kleinschnitz C., Schmidt H.H.
Q1 Journal of Clinical Investigation 2019 цитирований: 30
Amide Proton Transfer MRI Signal as a Surrogate Biomarker of Ischemic Stroke Recovery in Patients With Supportive Treatment
Yu L., Chen Y., Chen M., Luo X., Jiang S., Zhang Y., Chen H., Gong T., Zhou J., Li C.
Q2 Frontiers in Neurology 2019 цитирований: 6
Open Access
Open access
SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range
Ermakova Y.G., Pak V.V., Bogdanova Y.A., Kotlobay A.A., Yampolsky I.V., Shokhina A.G., Panova A.S., Marygin R.A., Staroverov D.B., Bilan D.S., Sies H., Belousov V.V.
Q1 Chemical Communications 2018 цитирований: 27
Reactive Oxygen Species Formation in the Brain at Different Oxygen Levels: The Role of Hypoxia Inducible Factors
Chen R., Lai U.H., Zhu L., Singh A., Ahmed M., Forsyth N.R.
Q1 Frontiers in Cell and Developmental Biology 2018 цитирований: 72
Open Access
Open access
Attenuation of oxidative damage by targeting mitochondrial complex I in neonatal hypoxic-ischemic brain injury
Kim M., Stepanova A., Niatsetskaya Z., Sosunov S., Arndt S., Murphy M.P., Galkin A., Ten V.S.
Q1 Free Radical Biology and Medicine 2018 цитирований: 30
Метрики
Поделиться
Цитировать
ГОСТ |
Цитировать
1. Kelmanson I.V. и др. In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model // Redox Biology. 2021. Т. 48. С. 102178.
RIS |
Цитировать

TY - JOUR

DO - 10.1016/j.redox.2021.102178

UR - http://dx.doi.org/10.1016/j.redox.2021.102178

TI - In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

T2 - Redox Biology

AU - Kelmanson, Ilya V.

AU - Shokhina, Arina G.

AU - Kotova, Daria A.

AU - Pochechuev, Matvei S.

AU - Ivanova, Alexandra D.

AU - Kostyuk, Alexander I.

AU - Panova, Anastasiya S.

AU - Borodinova, Anastasia A.

AU - Solotenkov, Maxim A.

AU - Stepanov, Evgeny A.

AU - Raevskii, Roman I.

AU - Moshchenko, Aleksandr A.

AU - Pak, Valeriy V.

AU - Ermakova, Yulia G.

AU - van Belle, Gijsbert J.C.

AU - Tarabykin, Viktor

AU - Balaban, Pavel M.

AU - Fedotov, Ilya V.

AU - Fedotov, Andrei B.

AU - Conrad, Marcus

AU - Bogeski, Ivan

AU - Katschinski, Dörthe M.

AU - Doeppner, Thorsten R.

AU - Bähr, Mathias

AU - Zheltikov, Aleksei M.

AU - Belousov, Vsevolod V.

AU - Bilan, Dmitry S.

PY - 2021

DA - 2021/12

PB - Elsevier BV

SP - 102178

VL - 48

SN - 2213-2317

ER -

BibTex |
Цитировать

@article{Kelmanson_2021,

doi = {10.1016/j.redox.2021.102178},

url = {https://doi.org/10.1016%2Fj.redox.2021.102178},

year = 2021,

month = {dec},

publisher = {Elsevier {BV}},

volume = {48},

pages = {102178},

author = {Ilya V. Kelmanson and Arina G. Shokhina and Daria A. Kotova and Matvei S. Pochechuev and Alexandra D. Ivanova and Alexander I. Kostyuk and Anastasiya S. Panova and Anastasia A. Borodinova and Maxim A. Solotenkov and Evgeny A. Stepanov and Roman I. Raevskii and Aleksandr A. Moshchenko and Valeriy V. Pak and Yulia G. Ermakova and Gijsbert J.C. van Belle and Viktor Tarabykin and Pavel M. Balaban and Ilya V. Fedotov and Andrei B. Fedotov and Marcus Conrad and Ivan Bogeski and Dörthe M. Katschinski and Thorsten R. Doeppner and Mathias Bähr and Aleksei M. Zheltikov and Vsevolod V. Belousov and Dmitry S. Bilan},

title = {In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model},

journal = {Redox Biology}

}

MLA
Цитировать
Kelmanson, Ilya V. et al. “In Vivo Dynamics of Acidosis and Oxidative Stress in the Acute Phase of an Ischemic Stroke in a Rodent Model.” Redox Biology 48 (2021): 102178. Crossref. Web.