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Biology, volume 10, issue 11, pages 1172

Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii

Publication typeJournal Article
Publication date2021-11-12
Journal: Biology
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.2
ISSN20797737
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Immunology and Microbiology
Abstract

The Siberian salamander Salamandrella keyserlingii Dybowski, 1870 is a unique amphibian that is capable to survive long-term freezing at −55 °C. Nothing is known on the biochemical basis of this remarkable freezing tolerance, except for the fact that it uses glycerol as a low molecular weight cryoprotectant. We used 1H-NMR analysis to study quantitative changes of multiple metabolites in liver and hindlimb muscle of S. keyserlingii in response to freezing. For the majority of molecules we observed significant changes in concentrations. Glycerol content in frozen organs was as high as 2% w/w, which confirms its role as a cryoprotectant. No other putative cryoprotectants were detected. Freezing resulted in ischemia manifested as increased concentrations of glycolysis products: lactate and alanine. Unexpectedly, we detected no increase in concentrations of succinate, which accumulates under ischemia in various tetrapods. Freezing proved to be a dramatic stress with reduced adenosine phosphate pool and high levels of nucleotide degradation products (hypoxanthine, β-alanine, and β-aminoisobutyrate). There was also significant increase in the concentrations of choline and glycerophosphocholine, which may be interpreted as the degradation of biomembranes. Thus, we found that freezing results not only in macroscopical damage due to ice formation, but also to degradation of DNA and biomembranes.

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GOST Copy
Shekhovtsov S. V. et al. Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii // Biology. 2021. Vol. 10. No. 11. p. 1172.
GOST all authors (up to 50) Copy
Shekhovtsov S. V., Bulakhova N. A., Tsentalovich Y. P., Zelentsova E. A., Meshcheryakova E. N., Poluboyarova T. V., Berman D. I. Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii // Biology. 2021. Vol. 10. No. 11. p. 1172.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/biology10111172
UR - https://doi.org/10.3390%2Fbiology10111172
TI - Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii
T2 - Biology
AU - Bulakhova, Nina A
AU - Zelentsova, Ekaterina A
AU - Meshcheryakova, Ekaterina N
AU - Poluboyarova, Tatiana V
AU - Berman, Daniil I
AU - Shekhovtsov, Sergei V.
AU - Tsentalovich, Yuri P
PY - 2021
DA - 2021/11/12 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1172
IS - 11
VL - 10
PMID - 34827165
SN - 2079-7737
ER -
BibTex |
Cite this
BibTex Copy
@article{2021_Shekhovtsov
author = {Nina A Bulakhova and Ekaterina A Zelentsova and Ekaterina N Meshcheryakova and Tatiana V Poluboyarova and Daniil I Berman and Sergei V. Shekhovtsov and Yuri P Tsentalovich},
title = {Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii},
journal = {Biology},
year = {2021},
volume = {10},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fbiology10111172},
number = {11},
pages = {1172},
doi = {10.3390/biology10111172}
}
MLA
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MLA Copy
Shekhovtsov, Sergei V., et al. “Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii.” Biology, vol. 10, no. 11, Nov. 2021, p. 1172. https://doi.org/10.3390%2Fbiology10111172.
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