Open Access
International Journal of Molecular Sciences, volume 22, issue 22, pages 12174
Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex
Publication type: Journal Article
Publication date: 2021-11-10
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
PubMed ID:
34830051
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Epileptic activity leads to rapid insertion of calcium-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (CP-AMPARs) into the synapses of cortical and hippocampal glutamatergic neurons, which generally do not express them. The physiological significance of this process is not yet fully understood; however, it is usually assumed to be a pathological process that augments epileptic activity. Using whole-cell patch-clamp recordings in rat entorhinal cortex slices, we demonstrate that the timing of epileptiform discharges, induced by 4-aminopyridine and gabazine, is determined by the shunting effect of Ca2+-dependent slow conductance, mediated predominantly by K+-channels. The blockade of CP-AMPARs by IEM-1460 eliminates this extra conductance and consequently increases the rate of discharge generation. The blockade of NMDARs reduced the additional conductance to a lesser extent than the blockade of CP-AMPARs, indicating that CP-AMPARs are a more significant source of intracellular Ca2+. The study’s main findings were implemented in a mathematical model, which reproduces the shunting effect of activity-dependent conductance on the generation of discharges. The obtained results suggest that the expression of CP-AMPARs in principal neurons reduces the discharge generation rate and may be considered as a protective mechanism.
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
3 publications, 100%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 100%
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Amakhin D. V. et al. Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex // International Journal of Molecular Sciences. 2021. Vol. 22. No. 22. p. 12174.
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Amakhin D. V., Soboleva E. B., Chizhov A. V., Zaitsev A. V. Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex // International Journal of Molecular Sciences. 2021. Vol. 22. No. 22. p. 12174.
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TY - JOUR
DO - 10.3390/ijms222212174
UR - https://doi.org/10.3390%2Fijms222212174
TI - Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex
T2 - International Journal of Molecular Sciences
AU - Soboleva, Elena B
AU - Chizhov, Anton V
AU - Amakhin, Dmitry V
AU - Zaitsev, A. V.
PY - 2021
DA - 2021/11/10 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 12174
IS - 22
VL - 22
PMID - 34830051
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2021_Amakhin,
author = {Elena B Soboleva and Anton V Chizhov and Dmitry V Amakhin and A. V. Zaitsev},
title = {Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex},
journal = {International Journal of Molecular Sciences},
year = {2021},
volume = {22},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fijms222212174},
number = {22},
pages = {12174},
doi = {10.3390/ijms222212174}
}
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MLA
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Amakhin, Dmitry V., et al. “Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex.” International Journal of Molecular Sciences, vol. 22, no. 22, Nov. 2021, p. 12174. https://doi.org/10.3390%2Fijms222212174.
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