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Open access
Molecules, volume 26, issue 21, pages 6564

Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications

Publication typeJournal Article
Publication date2021-10-29
Journal: Molecules
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.6
ISSN14203049
Organic Chemistry
Drug Discovery
Physical and Theoretical Chemistry
Pharmaceutical Science
Molecular Medicine
Analytical Chemistry
Chemistry (miscellaneous)
Abstract

Background: Tumor-targeting bacteriophages can be used as a versatile new platform for the delivery of diagnostic imaging agents and therapeutic cargo. This became possible due to the development of viral capsid modification method. Earlier in our laboratory and using phage display technology, phages to malignant breast cancer cells MDA-MB 231 were obtained. The goal of this study was the optimization of phage modification and the assessment of the effect of the latter on the efficiency of phage particle penetration into MDA-MB 231 cells. Methods: In this work, we used several methods, such as chemical phage modification using FAM-NHS ester, spectrophotometry, phage amplification, sequencing, phage titration, flow cytometry, and confocal microscopy. Results: We performed chemical phage modification using different concentrations of FAM-NHS dye (0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM). It was shown that with an increase of the modification degree, the phage titer decreases. The maximum modification coefficient of the phage envelope with the FAM–NHS dye was observed with 4 mM modifying agent and had approximately 804,2 FAM molecules per phage. Through the immunofluorescence staining and flow cytometry methods, it was shown that the modified bacteriophage retains the ability to internalize into MDA-MB-231 cells. The estimation of the number of phages that could have penetrated into one tumor cell was conducted. Conclusions: Optimizing the conditions for phage modification can be an effective strategy for producing tumor-targeting diagnostic and therapeutic agents, i.e., theranostic drugs.

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Дымова М. А. et al. Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications // Molecules. 2021. Vol. 26. No. 21. p. 6564.
GOST all authors (up to 50) Copy
Дымова М. А., Utkin Y. A., Dmitrieva M. D., Kuligina E. V., Richter V. A. Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications // Molecules. 2021. Vol. 26. No. 21. p. 6564.
RIS |
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RIS Copy
TY - JOUR
DO - 10.3390/molecules26216564
UR - https://doi.org/10.3390%2Fmolecules26216564
TI - Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications
T2 - Molecules
AU - Utkin, Yaroslav Alexandrovich
AU - Richter, Vladimir Alexandrovich
AU - Dmitrieva, Maria Denisovna
AU - Дымова, М. А.
AU - Kuligina, Elena Vladimirovna
PY - 2021
DA - 2021/10/29 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 6564
IS - 21
VL - 26
PMID - 34770973
SN - 1420-3049
ER -
BibTex |
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BibTex Copy
@article{2021_Дымова
author = {Yaroslav Alexandrovich Utkin and Vladimir Alexandrovich Richter and Maria Denisovna Dmitrieva and М. А. Дымова and Elena Vladimirovna Kuligina},
title = {Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications},
journal = {Molecules},
year = {2021},
volume = {26},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {oct},
url = {https://doi.org/10.3390%2Fmolecules26216564},
number = {21},
pages = {6564},
doi = {10.3390/molecules26216564}
}
MLA
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MLA Copy
Дымова, М. А., et al. “Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications.” Molecules, vol. 26, no. 21, Oct. 2021, p. 6564. https://doi.org/10.3390%2Fmolecules26216564.
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