Open Access
Nature Communications, volume 13, issue 1, publication number 171
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
Kostyuk Alexander I
1, 2, 3
,
Tossounian Maria-Armineh
4, 5, 6, 7
,
Panova Anastasiya S.
1, 2, 3
,
Thauvin Marion
8, 9
,
Raevskii Roman I
3
,
Ezeriņa Daria
5, 6, 7
,
Wahni Khadija
5, 6, 7
,
Van Molle Inge
5, 7
,
Sergeeva Anastasia D
3, 10
,
Vertommen Didier
11
,
Gorokhovatsky Andrey Yu.
3
,
Baranov Mikhail S
3, 12
,
Vriz Sophie
9, 13, 14
,
Messens Joris
5, 6, 7
,
Bilan Dmitry S.
1, 2, 3
,
Belousov Vsevolod V
1, 2, 3, 15
4
5
Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium
|
6
Brussels Center for Redox Biology, Vrije Universiteit Brussel, Brussels, Belgium
|
9
Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France
|
14
Université de paris, Paris, France
|
Publication type: Journal Article
Publication date: 2022-01-10
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723
General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy
Abstract
The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M−1s−1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model.
Citations by journals
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1
2
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Free Radical Biology and Medicine
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2 publications, 22.22%
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Molecular Microbiology
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1 publication, 11.11%
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Antioxidants
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1 publication, 11.11%
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Nature Reviews Molecular Cell Biology
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1 publication, 11.11%
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Nature Metabolism
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Nature Metabolism
1 publication, 11.11%
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ACS Omega
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ACS Omega
1 publication, 11.11%
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Protein Science
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Protein Science
1 publication, 11.11%
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Biochemistry (Moscow)
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1 publication, 11.11%
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1
2
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Citations by publishers
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1
2
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Wiley
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Wiley
2 publications, 22.22%
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Springer Nature
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Springer Nature
2 publications, 22.22%
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Elsevier
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Elsevier
2 publications, 22.22%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 11.11%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 11.11%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 11.11%
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1
2
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Kostyuk A. I. et al. Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives // Nature Communications. 2022. Vol. 13. No. 1. 171
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Kostyuk A. I., Tossounian M., Panova A. S., Thauvin M., Raevskii R. I., Ezeriņa D., Wahni K., Van Molle I., Sergeeva A. D., Vertommen D., Gorokhovatsky A. Y., Baranov M. S., Vriz S., Messens J., Bilan D. S., Belousov V. V. Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives // Nature Communications. 2022. Vol. 13. No. 1. 171
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TY - JOUR
DO - 10.1038/s41467-021-27796-2
UR - https://doi.org/10.1038%2Fs41467-021-27796-2
TI - Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
T2 - Nature Communications
AU - Kostyuk, Alexander I
AU - Tossounian, Maria-Armineh
AU - Thauvin, Marion
AU - Raevskii, Roman I
AU - Ezeriņa, Daria
AU - Wahni, Khadija
AU - Van Molle, Inge
AU - Vertommen, Didier
AU - Gorokhovatsky, Andrey Yu.
AU - Baranov, Mikhail S
AU - Vriz, Sophie
AU - Messens, Joris
AU - Bilan, Dmitry S.
AU - Belousov, Vsevolod V
AU - Sergeeva, Anastasia D
AU - Panova, Anastasiya S.
PY - 2022
DA - 2022/01/10 00:00:00
PB - Springer Nature
IS - 1
VL - 13
SN - 2041-1723
ER -
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@article{2022_Kostyuk,
author = {Alexander I Kostyuk and Maria-Armineh Tossounian and Marion Thauvin and Roman I Raevskii and Daria Ezeriņa and Khadija Wahni and Inge Van Molle and Didier Vertommen and Andrey Yu. Gorokhovatsky and Mikhail S Baranov and Sophie Vriz and Joris Messens and Dmitry S. Bilan and Vsevolod V Belousov and Anastasia D Sergeeva and Anastasiya S. Panova},
title = {Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives},
journal = {Nature Communications},
year = {2022},
volume = {13},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1038%2Fs41467-021-27796-2},
number = {1},
doi = {10.1038/s41467-021-27796-2}
}