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Frontiers in Microbiology, volume 12

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu Shaowei 1, 2
Wang Ting 2
Lu Qinpei 2
Li Feina 2
Wu Gang 2
Jiang Zhongke 2
Habden Xugela 3
Liu Lin 4
Zhang Xiaolin 5
Osterman Ilya A. 6, 7
Sergiev Petr V. 6, 7
Dontsova Olga A. 6, 7, 8
Sun Chenghang 1, 2
Publication typeJournal Article
Publication date2021-03-15
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor5.2
ISSN1664302X
Microbiology (medical)
Microbiology
Abstract

Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter “pDualrep2” system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested “ESKAPE” pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably,Saccharothrixsp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (116) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds811displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.

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Liu S. et al. Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert // Frontiers in Microbiology. 2021. Vol. 12.
GOST all authors (up to 50) Copy
Liu S., Wang T., Lu Q., Li F., Wu G., Jiang Z., Habden X., Liu L., Zhang X., Lukianov D. A., Osterman I. A., Sergiev P. V., Dontsova O. A., Sun C. Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert // Frontiers in Microbiology. 2021. Vol. 12.
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RIS Copy
TY - JOUR
DO - 10.3389/fmicb.2021.604999
UR - https://doi.org/10.3389%2Ffmicb.2021.604999
TI - Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert
T2 - Frontiers in Microbiology
AU - Liu, Shaowei
AU - Wang, Ting
AU - Lu, Qinpei
AU - Li, Feina
AU - Wu, Gang
AU - Jiang, Zhongke
AU - Habden, Xugela
AU - Liu, Lin
AU - Zhang, Xiaolin
AU - Lukianov, Dmitry A
AU - Osterman, Ilya A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
AU - Sun, Chenghang
PY - 2021
DA - 2021/03/15 00:00:00
PB - Frontiers Media S.A.
VL - 12
PMID - 33790875
SN - 1664-302X
ER -
BibTex
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BibTex Copy
@article{2021_Liu
author = {Shaowei Liu and Ting Wang and Qinpei Lu and Feina Li and Gang Wu and Zhongke Jiang and Xugela Habden and Lin Liu and Xiaolin Zhang and Dmitry A Lukianov and Ilya A. Osterman and Petr V. Sergiev and Olga A. Dontsova and Chenghang Sun},
title = {Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert},
journal = {Frontiers in Microbiology},
year = {2021},
volume = {12},
publisher = {Frontiers Media S.A.},
month = {mar},
url = {https://doi.org/10.3389%2Ffmicb.2021.604999},
doi = {10.3389/fmicb.2021.604999}
}
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