Journal of Physical Chemistry B, volume 120, issue 16, pages 3873-3879

Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2

Publication typeJournal Article
Publication date2016-04-13
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor3.3
ISSN15206106, 15205207
Materials Chemistry
Surfaces, Coatings and Films
Physical and Theoretical Chemistry
Abstract
Complexes of small GTPases with GTPase-activating proteins have been intensively studied with the main focus on the complex of H-Ras with p120GAP (Ras-GAP). The detailed mechanism of GTP hydrolysis is still unresolved. To clarify it, we calculated the energy profile of GTP hydrolysis in the active site of a recently characterized vision-related member of this family, the Arl3-RP2 complex. The mechanism suggested in this study retains the main features of GTP hydrolysis by the Ras-GAP complex, but the relative energies of the corresponding intermediates are different and an additional intermediate exists in the Arl3-RP2 complex compared with the Ras-GAP. These differences arise from small deviations in the catalytic arginine conformation of the active site. In the Arl3-RP2 complex, the first two intermediates, corresponding to the Pγ-Oβγ bond cleavage and the glutamine-assisted proton transfer, are almost isoenergetic with the ES complex. Numerical simulations of the kinetic curves demonstrate that the concentrations of these intermediates are comparable with that of ES during the reaction. The calculated IR spectra reveal specific vibrational bands, corresponding to these intermediates. These specific features of the Arl3-RP2 complex open the opportunity to identify spectroscopically two more reaction intermediates in GTP hydrolysis in addition to the ES and EP complexes.

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Khrenova M. G. et al. Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2 // Journal of Physical Chemistry B. 2016. Vol. 120. No. 16. pp. 3873-3879.
GOST all authors (up to 50) Copy
Khrenova M. G., Kots E., Nemukhin A. Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2 // Journal of Physical Chemistry B. 2016. Vol. 120. No. 16. pp. 3873-3879.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1021/acs.jpcb.6b03363
UR - https://doi.org/10.1021%2Facs.jpcb.6b03363
TI - Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2
T2 - Journal of Physical Chemistry B
AU - Kots, Ekaterina D.
AU - Khrenova, Maria G.
AU - Nemukhin, Alexander
PY - 2016
DA - 2016/04/13 00:00:00
PB - American Chemical Society (ACS)
SP - 3873-3879
IS - 16
VL - 120
SN - 1520-6106
SN - 1520-5207
ER -
BibTex |
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BibTex Copy
@article{2016_Khrenova
author = {Ekaterina D. Kots and Maria G. Khrenova and Alexander Nemukhin},
title = {Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2},
journal = {Journal of Physical Chemistry B},
year = {2016},
volume = {120},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021%2Facs.jpcb.6b03363},
number = {16},
pages = {3873--3879},
doi = {10.1021/acs.jpcb.6b03363}
}
MLA
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MLA Copy
Khrenova, Maria G., et al. “Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2.” Journal of Physical Chemistry B, vol. 120, no. 16, Apr. 2016, pp. 3873-3879. https://doi.org/10.1021%2Facs.jpcb.6b03363.
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