Journal of Physical Chemistry B, volume 120, issue 16, pages 3873-3879
Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2
Publication type: Journal Article
Publication date: 2016-04-13
Journal:
Journal of Physical Chemistry B
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 3.3
ISSN: 15206106, 15205207
Materials Chemistry
Surfaces, Coatings and Films
Physical and Theoretical Chemistry
Abstract
Complexes of small GTPases with GTPase-activating proteins have been intensively studied with the main focus on the complex of H-Ras with p120GAP (Ras-GAP). The detailed mechanism of GTP hydrolysis is still unresolved. To clarify it, we calculated the energy profile of GTP hydrolysis in the active site of a recently characterized vision-related member of this family, the Arl3-RP2 complex. The mechanism suggested in this study retains the main features of GTP hydrolysis by the Ras-GAP complex, but the relative energies of the corresponding intermediates are different and an additional intermediate exists in the Arl3-RP2 complex compared with the Ras-GAP. These differences arise from small deviations in the catalytic arginine conformation of the active site. In the Arl3-RP2 complex, the first two intermediates, corresponding to the Pγ-Oβγ bond cleavage and the glutamine-assisted proton transfer, are almost isoenergetic with the ES complex. Numerical simulations of the kinetic curves demonstrate that the concentrations of these intermediates are comparable with that of ES during the reaction. The calculated IR spectra reveal specific vibrational bands, corresponding to these intermediates. These specific features of the Arl3-RP2 complex open the opportunity to identify spectroscopically two more reaction intermediates in GTP hydrolysis in addition to the ES and EP complexes.
Citations by journals
1
|
|
Physical Chemistry Chemical Physics
|
Physical Chemistry Chemical Physics
1 publication, 16.67%
|
Molecules
|
Molecules
1 publication, 16.67%
|
ACS Catalysis
|
ACS Catalysis
1 publication, 16.67%
|
Biophysical Journal
|
Biophysical Journal
1 publication, 16.67%
|
Advances in Colloid and Interface Science
|
Advances in Colloid and Interface Science
1 publication, 16.67%
|
Russian Journal of Physical Chemistry A
|
Russian Journal of Physical Chemistry A
1 publication, 16.67%
|
1
|
Citations by publishers
1
|
|
Royal Society of Chemistry (RSC)
|
Royal Society of Chemistry (RSC)
1 publication, 16.67%
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 16.67%
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
1 publication, 16.67%
|
Biophysical Society
|
Biophysical Society
1 publication, 16.67%
|
Elsevier
|
Elsevier
1 publication, 16.67%
|
Pleiades Publishing
|
Pleiades Publishing
1 publication, 16.67%
|
1
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2017,2018,2019,2020,2021,2022,2023],"ids":[0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0],"imageUrls":["","","","","","",""],"datasets":[{"label":"Citations number","data":[2,1,0,0,2,0,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["33.33","16.67",0,0,"33.33",0,"16.67"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Physical Chemistry Chemical Physics","Molecules","ACS Catalysis","Biophysical Journal","Advances in Colloid and Interface Science","Russian Journal of Physical Chemistry A"],"ids":[1773,1770,4873,4026,14365,6134],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/Bmz9XNb2QDptmPTa5N8huM54tShVewUj8fZqKFRy_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.67,16.67,16.67,16.67,16.67,16.67],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Royal Society of Chemistry (RSC)","Multidisciplinary Digital Publishing Institute (MDPI)","American Chemical Society (ACS)","Biophysical Society","Elsevier","Pleiades Publishing"],"ids":[123,202,40,1454,17,101],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/Bmz9XNb2QDptmPTa5N8huM54tShVewUj8fZqKFRy_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.67,16.67,16.67,16.67,16.67,16.67],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Khrenova M. G. et al. Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2 // Journal of Physical Chemistry B. 2016. Vol. 120. No. 16. pp. 3873-3879.
GOST all authors (up to 50)
Copy
Khrenova M. G., Kots E., Nemukhin A. Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2 // Journal of Physical Chemistry B. 2016. Vol. 120. No. 16. pp. 3873-3879.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1021/acs.jpcb.6b03363
UR - https://doi.org/10.1021%2Facs.jpcb.6b03363
TI - Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2
T2 - Journal of Physical Chemistry B
AU - Kots, Ekaterina D.
AU - Khrenova, Maria G.
AU - Nemukhin, Alexander
PY - 2016
DA - 2016/04/13 00:00:00
PB - American Chemical Society (ACS)
SP - 3873-3879
IS - 16
VL - 120
SN - 1520-6106
SN - 1520-5207
ER -
Cite this
BibTex
Copy
@article{2016_Khrenova
author = {Ekaterina D. Kots and Maria G. Khrenova and Alexander Nemukhin},
title = {Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2},
journal = {Journal of Physical Chemistry B},
year = {2016},
volume = {120},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021%2Facs.jpcb.6b03363},
number = {16},
pages = {3873--3879},
doi = {10.1021/acs.jpcb.6b03363}
}
Cite this
MLA
Copy
Khrenova, Maria G., et al. “Reaction Mechanism of Guanosine Triphosphate Hydrolysis by the Vision-Related Protein Complex Arl3-RP2.” Journal of Physical Chemistry B, vol. 120, no. 16, Apr. 2016, pp. 3873-3879. https://doi.org/10.1021%2Facs.jpcb.6b03363.
Profiles