Journal of Physical Chemistry B, volume 117, issue 24, pages 7228-7234
Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family
Publication type: Journal Article
Publication date: 2013-06-06
Journal:
Journal of Physical Chemistry B
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 3.3
ISSN: 15206106, 15205207
Materials Chemistry
Surfaces, Coatings and Films
Physical and Theoretical Chemistry
Abstract
The kindling fluorescent protein (KFP), the Ala143Gly variant of the natural chromoprotein asFP595, is a prospective biomarker in live cells. Following the results of QM/MM calculations, we predict that excitation of the protein under certain conditions, favoring formation of KFP fractions with the neutral chromophore, should result in fluorescence from the cationic form of the chromophore which is unusual for the members of the green fluorescent protein family. Occurrence of the neutral form is due to a water wire connecting the chromophore with the exterior of the protein. Occurrence of the cationic form is due to the excited-state proton transfer from the conserved Glu215 to the imidazolinone ring nitrogen of the chromophore. The emission band from conformations with the trans cationic chromophore should be noticeably shifted to the blue side around 520 nm compared to the well-known red fluorescence around 600 nm arising from the cis anionic species.
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1 publication, 7.69%
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1 publication, 7.69%
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1 publication, 7.69%
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Grigorenko B. et al. Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family // Journal of Physical Chemistry B. 2013. Vol. 117. No. 24. pp. 7228-7234.
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Grigorenko B., Polyakov I. V., Savitsky A., Nemukhin A. Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family // Journal of Physical Chemistry B. 2013. Vol. 117. No. 24. pp. 7228-7234.
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TY - JOUR
DO - 10.1021/jp402149q
UR - https://doi.org/10.1021%2Fjp402149q
TI - Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family
T2 - Journal of Physical Chemistry B
AU - Polyakov, Igor V.
AU - Grigorenko, Bella
AU - Savitsky, Alexander
AU - Nemukhin, Alexander
PY - 2013
DA - 2013/06/06 00:00:00
PB - American Chemical Society (ACS)
SP - 7228-7234
IS - 24
VL - 117
SN - 1520-6106
SN - 1520-5207
ER -
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@article{2013_Grigorenko,
author = {Igor V. Polyakov and Bella Grigorenko and Alexander Savitsky and Alexander Nemukhin},
title = {Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family},
journal = {Journal of Physical Chemistry B},
year = {2013},
volume = {117},
publisher = {American Chemical Society (ACS)},
month = {jun},
url = {https://doi.org/10.1021%2Fjp402149q},
number = {24},
pages = {7228--7234},
doi = {10.1021/jp402149q}
}
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MLA
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Grigorenko, Bella, et al. “Unusual emitting states of the kindling fluorescent protein: Appearance of the cationic chromophore in the GFP family.” Journal of Physical Chemistry B, vol. 117, no. 24, Jun. 2013, pp. 7228-7234. https://doi.org/10.1021%2Fjp402149q.