Open Access
Nature Communications, volume 10, issue 1, publication number 3177
Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
Nass Kovacs Gabriela
1
,
Colletier Jacques-Philippe
2
,
Grünbein Marie Luise
1
,
Yang Yang
3
,
Stensitzki T
3
,
Batyuk Alexander
4
,
Carbajo Sergio
4
,
Doak R. Bruce
1
,
Ehrenberg David
3
,
Foucar Lutz
1
,
Gasper Raphael
5
,
Gorel Alexander
1
,
Hilpert Mario
1
,
Kloos Marco
1
,
Koglin Jason E.
4
,
Reinstein Jochen
1
,
Roome Christopher M.
1
,
Schlesinger R.
3
,
Seaberg M. H.
4
,
Shoeman Robert
1
,
Stricker Miriam
1
,
Boutet Sébastien
4
,
Haacke Sven
6
,
Heberle Joachim
3
,
Heyne Karsten
3
,
Barends Thomas R. M.
1
,
4
Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, CA, USA
|
5
Max-Planck-Institut für molekulare Physiologie, Dortmund, Germany
|
Publication type: Journal Article
Publication date: 2019-07-18
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723
PubMed ID:
31320619
General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy
Abstract
Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking the retinal chromophore, as a first manifestation of multiphoton effects. We resolve distinct stages in the structural dynamics of the all-trans retinal in photoexcited bR to a highly twisted 13-cis conformation. Other active site sub-picosecond rearrangements include correlated vibrational motions of the electronically excited retinal chromophore, the surrounding amino acids and water molecules as well as their hydrogen bonding network. These results show that this extended photo-active network forms an electronically and vibrationally coupled system in bR, and most likely in all retinal proteins.Bacteriorhodopsin (bR) is a light-driven proton pump. Here the authors combine time-resolved crystallography at a free-electron laser, ultrafast spectroscopy and quantum chemistry to study the structural changes following multiphoton photoexcitation of bR and find that they occur within 300 fs not only in the light-absorbing chromophore but also in the surrounding protein.
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5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Nass Kovacs G. et al. Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin // Nature Communications. 2019. Vol. 10. No. 1. 3177
GOST all authors (up to 50)
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Nass Kovacs G., Colletier J., Grünbein M. L., Yang Y., Stensitzki T., Batyuk A., Carbajo S., Doak R. B., Ehrenberg D., Foucar L., Gasper R., Gorel A., Hilpert M., Kloos M., Koglin J. E., Reinstein J., Roome C. M., Schlesinger R., Seaberg M. H., Shoeman R., Stricker M., Boutet S., Haacke S., Heberle J., Heyne K., Domratcheva T., Barends T., Schlichting I. Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin // Nature Communications. 2019. Vol. 10. No. 1. 3177
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TY - JOUR
DO - 10.1038/s41467-019-10758-0
UR - https://doi.org/10.1038%2Fs41467-019-10758-0
TI - Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
T2 - Nature Communications
AU - Nass Kovacs, Gabriela
AU - Colletier, Jacques-Philippe
AU - Grünbein, Marie Luise
AU - Stensitzki, T
AU - Batyuk, Alexander
AU - Carbajo, Sergio
AU - Doak, R. Bruce
AU - Ehrenberg, David
AU - Foucar, Lutz
AU - Gasper, Raphael
AU - Gorel, Alexander
AU - Hilpert, Mario
AU - Kloos, Marco
AU - Koglin, Jason E.
AU - Reinstein, Jochen
AU - Roome, Christopher M.
AU - Schlesinger, R.
AU - Seaberg, M. H.
AU - Shoeman, Robert
AU - Stricker, Miriam
AU - Boutet, Sébastien
AU - Haacke, Sven
AU - Heyne, Karsten
AU - Domratcheva, Tatiana
AU - Barends, Thomas R. M.
AU - Schlichting, Ilme
AU - Yang, Yang
AU - Heberle, Joachim
PY - 2019
DA - 2019/07/18 00:00:00
PB - Springer Nature
IS - 1
VL - 10
PMID - 31320619
SN - 2041-1723
ER -
Cite this
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@article{2019_Nass Kovacs,
author = {Gabriela Nass Kovacs and Jacques-Philippe Colletier and Marie Luise Grünbein and T Stensitzki and Alexander Batyuk and Sergio Carbajo and R. Bruce Doak and David Ehrenberg and Lutz Foucar and Raphael Gasper and Alexander Gorel and Mario Hilpert and Marco Kloos and Jason E. Koglin and Jochen Reinstein and Christopher M. Roome and R. Schlesinger and M. H. Seaberg and Robert Shoeman and Miriam Stricker and Sébastien Boutet and Sven Haacke and Karsten Heyne and Tatiana Domratcheva and Thomas R. M. Barends and Ilme Schlichting and Yang Yang and Joachim Heberle},
title = {Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin},
journal = {Nature Communications},
year = {2019},
volume = {10},
publisher = {Springer Nature},
month = {jul},
url = {https://doi.org/10.1038%2Fs41467-019-10758-0},
number = {1},
doi = {10.1038/s41467-019-10758-0}
}